FastQCFastQC Report
Thu 2 Feb 2017
SRR4062866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209875
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31871.518522930315664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25541.2169148302561048No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15630.7447290053603335No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15310.7294818344252532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14890.7094699225729602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.5069684335914234No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7740.36879094699225734No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT6760.32209648600357355No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6720.3201905896366885No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA6600.31447290053603333No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA5880.28016676593210244No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.27349612864800477No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.26968433591423463No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA5230.24919594997022038No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5160.24586063132817151No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA5090.24252531268612268No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4780.22775461584276357No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC4740.2258487194758785No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4710.22441929720071468No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.2144133412745682No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT4450.2120309708159619No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT4350.20726622989874927No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG4230.20154854079809412No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3930.18725431804645623No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG3870.18439547349612867No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT3790.18058368076235856No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA3780.1801072066706373No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3770.179630732578916No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3760.17915425848719477No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3720.1772483621203097No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC3680.17534246575342466No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3680.17534246575342466No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG3650.17391304347826086No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC3630.17296009529481834No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA3580.17057772483621203No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC3520.16771888028588444No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT3340.15914234663490173No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA3320.1581893984514592No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG3290.15675997617629542No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC3290.15675997617629542No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA3250.15485407980941035No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3230.15390113162596786No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC3230.15390113162596786No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG3190.1519952352590828No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC3140.14961286480047647No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC3130.1491363907087552No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC3110.14818344252531268No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT3020.14389517569982133No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA3010.14341870160810005No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT3000.14294222751637878No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT2990.14246575342465753No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT2950.1405598570577725No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA2880.13722453841572363No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG2860.1362715902322811No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC2810.13388921977367482No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA2800.13341274568195355No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA2800.13341274568195355No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA2710.12912447885646217No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG2710.12912447885646217No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT2680.1276950565812984No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2610.12435973793924954No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA2590.12340678975580703No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG2590.12340678975580703No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2530.12054794520547946No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT2490.1186420488385944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.1186420488385944No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA2480.11816557474687314No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA2470.11768910065515187No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG2460.11721262656343061No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGC2450.11673615247170936No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG2420.11530673019654557No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA2390.11387730792138179No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT2390.11387730792138179No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT2390.11387730792138179No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG2380.11340083382966051No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT2380.11340083382966051No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2360.112447885646218No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA2350.11197141155449672No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT2340.11149493746277547No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGA2310.11006551518761168No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTA2250.1072066706372841No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGA2240.10673019654556283No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGT2210.10530077427039905No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC2210.10530077427039905No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2210.10530077427039905No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC2200.10482430017867779No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA2170.10339487790351401No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2130.10148898153662894No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT2130.10148898153662894No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGAT2120.10101250744490768No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTA2100.10005955926146515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG200.003743169132.005964
TCTAAGG200.003743169132.005963
TAAGGGC200.003743169132.005965
AGCTAAT200.00374754131.99833310
GAACTTA453.8318103E-728.4429611
GAACAGT308.384719E-426.671636
ATTGAAC509.59757E-725.6047678
TGAACTT509.617561E-725.59866510
TCGATGT451.2323026E-524.88759219
CGATGTT451.2323026E-524.88759220
AATCTGA350.002061576122.85595126
AGTGTAT350.002061576122.85595110
CGACCTC502.7846832E-522.39883214
TTATAGC453.5190483E-421.3373058
TTGAACT604.682701E-621.3373059
AGATTAT453.5190483E-421.3373055
CCACGTC604.682701E-621.3373059
CTTGTTA453.5247215E-421.33222232
GTTTAGA453.5247215E-421.3322221
TAGCCAA453.5247215E-421.33222211
Produced by FastQC (version 0.11.5)