FastQCFastQC Report
Thu 2 Feb 2017
SRR4062865_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062865_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145461
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39580.34553773546196687No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT31340.27360163287968775No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT29950.2614667806236965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27900.24357005607349352No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27700.2418240341661567No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC26700.23309392462947232No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG23900.20864961792675615No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23550.20559407958891662No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23480.20498297192134868No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23340.20376075658621287No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC23070.2014036270113081No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22740.1985226908642023No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22740.1985226908642023No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC22420.19572905581246328No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22140.19328462514219164No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20450.1785307400251951No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC19650.1715466523958476No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT19170.16735619981823913No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG19030.1661339844831033No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18630.16264194066842957No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG18360.1602848110935248No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC17590.15356262675027785No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17100.14928487307730248No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16740.14614203364409614No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT16650.14535632378579452No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC16590.14483251721359347No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC16370.1429118931155229No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC16280.14212618325722132No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC16070.14029286025451762No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG15740.13741192410741176No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15660.13671351534447704No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15450.13488019234177331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15230.13295956824370275No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA15000.13095164305026535No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG14860.12972942771512955No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC14730.12859451347536058No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG14440.1260627817097221No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC14400.12571357732825472No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14100.12309454446724943No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13990.12213423241821414No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG13790.1203882105108773No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC13460.11750727436377144No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT13350.11654696231473616No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13270.11584855355180142No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA13100.11436443493056507No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC13030.11375332726299715No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12360.10790415387341866No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA12330.10764225058731813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.10528512101241334No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12010.10484861553557913No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA11760.10266608815140803No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT11710.10222958267457381No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGA11580.10109466843480484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTG604.733278E-621.32676
CGAATGT707.7846926E-720.57131623
AGGACGT555.8472506E-520.3573025
TATACCG400.004491547619.993785
CGAACGA2600.019.69305816
ACGAACG2600.019.69305815
ATAACGA2750.019.20073312
TAACGAA2700.018.96368813
CGCTTCG3900.018.8716932
CGCAAGA5400.018.3758872
GTCCAAT701.7726064E-518.3031981
CCGATAA2900.018.2067979
CTAGCGG4750.018.19016829
GGCATCG4600.018.08606726
GCATCGT4600.018.08606727
CAAGACG5950.018.011994
AAACGCT4100.017.95190429
ACGCTTC4100.017.9511231
GCGCAAG5450.017.9253791
CGATAAC2950.017.89898910
Produced by FastQC (version 0.11.5)