##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062865_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1145461
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.241904351173893	32.0	32.0	32.0	32.0	32.0
2	30.889260306549065	32.0	32.0	32.0	32.0	32.0
3	30.94511205532096	32.0	32.0	32.0	32.0	32.0
4	31.00455275212338	32.0	32.0	32.0	32.0	32.0
5	30.93178554311321	32.0	32.0	32.0	32.0	32.0
6	34.5823463216993	36.0	36.0	36.0	32.0	36.0
7	34.48994858838494	36.0	36.0	36.0	32.0	36.0
8	34.48136252565561	36.0	36.0	36.0	32.0	36.0
9	34.60543833443478	36.0	36.0	36.0	32.0	36.0
10	34.34127133093139	36.0	36.0	36.0	32.0	36.0
11	34.594289111545486	36.0	36.0	36.0	32.0	36.0
12	34.40056012382787	36.0	36.0	36.0	32.0	36.0
13	34.51624193228752	36.0	36.0	36.0	32.0	36.0
14	34.40273566712442	36.0	36.0	36.0	32.0	36.0
15	34.36020257346169	36.0	36.0	36.0	32.0	36.0
16	34.36346763442841	36.0	36.0	36.0	32.0	36.0
17	34.2721995772881	36.0	36.0	36.0	32.0	36.0
18	34.3015187771561	36.0	36.0	36.0	32.0	36.0
19	34.29016003163792	36.0	36.0	36.0	32.0	36.0
20	34.27838835193865	36.0	36.0	36.0	32.0	36.0
21	34.25025470094573	36.0	36.0	36.0	32.0	36.0
22	34.22986727614472	36.0	36.0	36.0	32.0	36.0
23	34.18484697427498	36.0	36.0	36.0	32.0	36.0
24	34.16316662025159	36.0	36.0	36.0	32.0	36.0
25	34.13317607496021	36.0	36.0	36.0	32.0	36.0
26	34.076542108373836	36.0	36.0	36.0	32.0	36.0
27	34.08926624302355	36.0	36.0	36.0	32.0	36.0
28	34.07036293684377	36.0	36.0	36.0	32.0	36.0
29	34.03986429917736	36.0	36.0	36.0	32.0	36.0
30	33.9983700885495	36.0	36.0	36.0	32.0	36.0
31	34.02441287830838	36.0	36.0	36.0	32.0	36.0
32	33.97347618120565	36.0	36.0	36.0	32.0	36.0
33	33.93294228262682	36.0	36.0	36.0	32.0	36.0
34	33.944672930811265	36.0	36.0	36.0	32.0	36.0
35	33.89293131760924	36.0	36.0	36.0	32.0	36.0
36	33.83638203308537	36.0	36.0	36.0	32.0	36.0
37	33.83098595238074	36.0	36.0	36.0	32.0	36.0
38	33.24778931801257	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	0.0
10	8.0
11	12.0
12	4.0
13	6.0
14	131.0
15	267.0
16	400.0
17	525.0
18	625.0
19	870.0
20	1178.0
21	1572.0
22	2285.0
23	3295.0
24	4972.0
25	7390.0
26	10903.0
27	15928.0
28	21752.0
29	29670.0
30	40087.0
31	53600.0
32	72575.0
33	110246.0
34	247276.0
35	519881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.0796156797891	17.87288273373623	11.596561676715798	24.450939909758873
2	17.013563751729535	19.947048259283587	37.07792343922517	25.961464549761708
3	18.848444102958126	24.600373694687946	28.419131771033424	28.132050431320504
4	11.71896894165921	15.434969552786084	36.712835846956374	36.13322565859834
5	14.269813000890471	37.551464040647424	32.86618476419954	15.312538194262567
6	33.5331662384533	36.470421849096034	17.170002889676347	12.826409022774316
7	30.40260646150327	31.0418250817793	20.414051635105864	18.14151682161156
8	29.297625927794822	31.24104070162328	19.35880669566235	20.102526674919552
9	27.750685314196915	13.734307614408186	19.048504530930803	39.46650254046409
10	16.783766842607935	25.683787620323052	30.020306287965166	27.51213924910385
11	38.27234107936996	21.679328401338868	21.522009767374456	18.52632075191672
12	24.90030032127392	24.816838943986593	27.64998603156866	22.63287470317083
13	28.68350190693061	19.116280856651905	26.149222559058966	26.050994677358517
14	24.00134106190101	20.631573800749283	24.395889156344595	30.971195981005117
15	25.16458154503627	27.568838703536656	22.706446252413382	24.560133499013688
16	26.14898497123554	25.915603879032982	23.72515687522537	24.210254274506106
17	23.945023423549287	26.08540754356169	24.5773064446414	25.392262588247622
18	25.474308214125134	24.10815388443389	26.6178338666236	23.799704034817378
19	25.09095226828453	25.265741268625895	25.68533963807676	23.957966825012814
20	25.709972795434943	23.610698832543505	25.237210536792514	25.442117835229038
21	28.642096890930695	23.608968465790284	24.155244802866445	23.593689840412573
22	26.50964786451344	24.02803254457243	25.27617399594721	24.186145594966924
23	24.348037148502335	23.68474587221886	26.11570796324963	25.851509016029173
24	24.250938618702524	25.53200034925347	25.615821182222998	24.601239849821006
25	24.681643230858427	24.5735451287747	25.364198048296625	25.380613592070244
26	24.059344817981223	26.285517695433008	26.313022871208542	23.342114615377227
27	25.937427970748267	24.427694854404873	25.104682512519993	24.530194662326867
28	24.120569233455562	24.574122577265584	26.67356382049939	24.631744368779465
29	23.65826762441185	24.9506473685727	26.654524595116335	24.736560411899113
30	23.50693886673325	25.633385482507908	26.974626167245198	23.885049483513644
31	24.736052215956565	25.421927942887923	24.693708818863676	25.148311022291836
32	24.615000467089406	24.906167413573183	24.630453705328573	25.848378414008838
33	23.84740302260405	24.54639112251936	25.728192636440628	25.878013218435964
34	25.40676369332266	24.91633349951673	25.854149186123365	23.822753621037247
35	26.194729673093043	24.07367630623079	26.225901089689856	23.505692930986307
36	23.46269840022492	25.691923240133907	25.422208950428104	25.42316940921307
37	26.094294320513377	25.295935740166765	24.843148382590474	23.766621556729383
38	23.746625597614084	24.684211812940244	25.899695125145367	25.669467464300304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	116.0
2	158.0
3	158.0
4	421.0
5	684.0
6	684.0
7	858.0
8	1032.0
9	1144.5
10	1257.0
11	1257.0
12	1660.5
13	2064.0
14	2798.0
15	3532.0
16	3532.0
17	5114.5
18	6697.0
19	6697.0
20	7863.5
21	9030.0
22	9787.0
23	10544.0
24	10544.0
25	12225.5
26	13907.0
27	13907.0
28	17426.0
29	20945.0
30	24708.0
31	28471.0
32	28471.0
33	35952.5
34	43434.0
35	43434.0
36	46226.0
37	49018.0
38	57023.0
39	65028.0
40	65028.0
41	67724.5
42	70421.0
43	81543.0
44	92665.0
45	92665.0
46	91320.5
47	89976.0
48	89976.0
49	94385.0
50	98794.0
51	100827.5
52	102861.0
53	102861.0
54	96705.0
55	90549.0
56	90549.0
57	87708.0
58	84867.0
59	74278.0
60	63689.0
61	63689.0
62	64583.0
63	65477.0
64	55491.0
65	45505.0
66	45505.0
67	39428.0
68	33351.0
69	33351.0
70	26920.0
71	20489.0
72	15373.0
73	10257.0
74	10257.0
75	7779.5
76	5302.0
77	5302.0
78	5242.5
79	5183.0
80	4221.5
81	3260.0
82	3260.0
83	3253.5
84	3247.0
85	3247.0
86	2288.0
87	1329.0
88	1069.5
89	810.0
90	810.0
91	602.0
92	394.0
93	346.0
94	298.0
95	298.0
96	263.5
97	229.0
98	229.0
99	431.0
100	633.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12353104994408365
2	0.056309206511614106
3	0.012309454446724944
4	0.0031428394332063685
5	8.730109536684357E-5
6	3.492043814673743E-4
7	0.0
8	2.619032861005307E-4
9	8.730109536684357E-5
10	2.619032861005307E-4
11	0.0013095164305026534
12	0.001833323002703715
13	0.01440468073552919
14	0.008730109536684356
15	0.024793511084183574
16	0.010825335825488603
17	0.021476069460243517
18	0.00488886134054324
19	0.0069840876293474855
20	0.005674571198844832
21	0.006111076675679049
22	0.006809485438613799
23	0.00977772268108648
24	0.014055476354061814
25	0.01789672455020293
26	0.01938084317143927
27	0.008119001869116451
28	0.005325366817377458
29	0.01152374458842335
30	0.0025317317656384634
31	0.005587270103477988
32	0.006111076675679049
33	0.007944399678382764
34	0.012222153351358098
35	0.015801498261398685
36	0.015277691689197624
37	0.009690421585719635
38	0.00637297996177958
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1145461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.46522595236708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5551147950401	33.70950588328186
2	13.709147043824057	11.917423477480757
3	4.2645754917984755	5.560822120258431
4	1.688963486323983	2.936447182334783
5	0.7830727098637342	1.70182161356798
6	0.430053834753382	1.1215432259542608
7	0.2515942566744951	0.7654920850272336
8	0.15415966075599585	0.5360467589999693
9	0.11969518184827285	0.46823203120003626
>10	0.7309318858778004	6.440531899927471
>50	0.12644902220794077	3.962430320485657
>100	0.14778604464504183	13.789899787413768
>500	0.0261746399490931	7.872353974311099
>1k	0.012281946437651375	9.217449639756728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3958	0.34553773546196687	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3134	0.27360163287968775	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2995	0.2614667806236965	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2790	0.24357005607349352	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2770	0.2418240341661567	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2670	0.23309392462947232	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2390	0.20864961792675615	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2355	0.20559407958891662	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2348	0.20498297192134868	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2334	0.20376075658621287	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2307	0.2014036270113081	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2274	0.1985226908642023	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2274	0.1985226908642023	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2242	0.19572905581246328	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2214	0.19328462514219164	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2045	0.1785307400251951	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1965	0.1715466523958476	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1917	0.16735619981823913	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1903	0.1661339844831033	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1863	0.16264194066842957	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1836	0.1602848110935248	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1759	0.15356262675027785	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1710	0.14928487307730248	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1674	0.14614203364409614	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1665	0.14535632378579452	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1659	0.14483251721359347	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1637	0.1429118931155229	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1628	0.14212618325722132	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	1607	0.14029286025451762	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1574	0.13741192410741176	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1566	0.13671351534447704	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1545	0.13488019234177331	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1523	0.13295956824370275	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA	1500	0.13095164305026535	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	1486	0.12972942771512955	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC	1473	0.12859451347536058	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG	1444	0.1260627817097221	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC	1440	0.12571357732825472	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1410	0.12309454446724943	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1399	0.12213423241821414	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	1379	0.1203882105108773	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC	1346	0.11750727436377144	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1335	0.11654696231473616	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1327	0.11584855355180142	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA	1310	0.11436443493056507	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1303	0.11375332726299715	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1236	0.10790415387341866	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA	1233	0.10764225058731813	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1206	0.10528512101241334	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1201	0.10484861553557913	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA	1176	0.10266608815140803	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1171	0.10222958267457381	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGA	1158	0.10109466843480484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.730109536684357E-5	0.0
8	0.0	0.0	0.0	8.730109536684357E-5	0.0
9	0.0	0.0	0.0	8.730109536684357E-5	0.0
10	0.0	0.0	0.0	8.730109536684357E-5	0.0
11	0.0	0.0	0.0	1.7460219073368714E-4	0.0
12	0.0	0.0	0.0	1.7460219073368714E-4	0.0
13	0.0	0.0	0.0	1.7460219073368714E-4	0.0
14	0.0	0.0	0.0	1.7460219073368714E-4	0.0
15	0.0	0.0	0.0	1.7460219073368714E-4	0.0
16	0.0	0.0	0.0	1.7460219073368714E-4	0.0
17	0.0	0.0	0.0	2.619032861005307E-4	0.0
18	0.0	0.0	0.0	3.492043814673743E-4	0.0
19	0.0	0.0	0.0	4.3650547683421784E-4	0.0
20	0.0	0.0	0.0	4.3650547683421784E-4	0.0
21	0.0	0.0	0.0	5.238065722010614E-4	0.0
22	0.0	0.0	0.0	6.984087629347486E-4	0.0
23	0.0	0.0	0.0	0.0013095164305026534	0.0
24	0.0	0.0	0.0	0.0023571295749047765	0.0
25	0.0	0.0	0.0	0.002619032861005307	0.0
26	0.0	0.0	0.0	0.003055538337839525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGTG	60	4.733278E-6	21.3267	6
CGAATGT	70	7.7846926E-7	20.571316	23
AGGACGT	55	5.8472506E-5	20.357302	5
TATACCG	40	0.0044915476	19.99378	5
CGAACGA	260	0.0	19.693058	16
ACGAACG	260	0.0	19.693058	15
ATAACGA	275	0.0	19.200733	12
TAACGAA	270	0.0	18.963688	13
CGCTTCG	390	0.0	18.87169	32
CGCAAGA	540	0.0	18.375887	2
GTCCAAT	70	1.7726064E-5	18.303198	1
CCGATAA	290	0.0	18.206797	9
CTAGCGG	475	0.0	18.190168	29
GGCATCG	460	0.0	18.086067	26
GCATCGT	460	0.0	18.086067	27
CAAGACG	595	0.0	18.01199	4
AAACGCT	410	0.0	17.951904	29
ACGCTTC	410	0.0	17.95112	31
GCGCAAG	545	0.0	17.925379	1
CGATAAC	295	0.0	17.898989	10
>>END_MODULE