FastQCFastQC Report
Thu 2 Feb 2017
SRR4062865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145461
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41910.3658788906824414No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT32170.2808476237951358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31610.27595876245459255No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT30440.2657445342966718No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28930.25256206889627847No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25170.21973685703834525No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC25090.2190384482754105No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC24660.21528450117463627No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23980.20934802668969085No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23710.2069908971147861No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22990.20070521824837334No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22660.1978242821012675No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG22440.19590365800319698No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC22140.19328462514219164No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21680.18926877475531687No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21570.18830846270628157No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG20990.18324499917500464No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC20580.17966565426496406No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT19220.16779270529507334No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC18420.16080861766572585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18360.1602848110935248No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT18250.1593244990444895No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG18080.15784038042325316No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC17870.15600705742054946No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC17560.1533007234641773No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17280.1508562927939057No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG16810.14675314131166403No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC16670.14553092597652822No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC16390.1430864953062566No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16190.14134047339891972No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG16140.14090396792208554No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16090.1404674624452513No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15940.13915794601474865No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC15880.1386341394425476No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG15750.13749922520277863No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15740.13741192410741176No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15450.13488019234177331No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC15000.13095164305026535No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG14970.13068973976416481No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG14930.13034053538269746No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC14920.1302532342873306No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14800.1292056211429285No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA14290.12475326527921947No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC13450.11741997326840459No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.11637236012400247No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13290.11602315574253509No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13020.11366602616763033No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG12930.11288031630932874No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12690.11078509002052449No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC12560.10965017578075552No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA12110.10572162648924754No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT12110.10572162648924754No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA12060.10528512101241334No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA11840.10336449691434278No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT11640.10161847500700591No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGA11570.10100736733943799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG957.2759576E-1121.896144
TCTAGCG5600.019.42812228
TAGCGGC5750.018.92129930
CTAGCGG5750.018.64304529
AGGCCCG4650.017.89205710
CGTCTCG450.00885143817.77736510
AGCGGCG6300.017.52340331
CATCGTT5150.017.39765528
CAAGACG6300.017.2709484
ATAATAC753.2318967E-517.0677623
CGGCATC5350.017.04633325
GCATCGT5400.016.88849827
GACTGTG1651.8189894E-1216.4859077
GCGGCGC6550.016.36603432
AAGACGG6650.016.3619525
CGTTTAT5550.016.1437731
CGCAAGA6150.016.1304112
GCGCAAG6300.016.0024221
GGCATCG5850.015.8628826
ATCGTTT5650.015.8580429
Produced by FastQC (version 0.11.5)