##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1145461
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203030919428947	32.0	32.0	32.0	32.0	32.0
2	31.31408664284511	32.0	32.0	32.0	32.0	32.0
3	31.39502348835971	32.0	32.0	32.0	32.0	32.0
4	31.49045144269425	32.0	32.0	32.0	32.0	32.0
5	31.416055195244535	32.0	32.0	32.0	32.0	32.0
6	34.946470460364864	36.0	36.0	36.0	36.0	36.0
7	34.957071432375265	36.0	36.0	36.0	36.0	36.0
8	34.90359863845212	36.0	36.0	36.0	36.0	36.0
9	35.01603284616412	36.0	36.0	36.0	36.0	36.0
10	34.85281559127722	36.0	36.0	36.0	32.0	36.0
11	35.03566860853403	36.0	36.0	36.0	36.0	36.0
12	34.91304723600367	36.0	36.0	36.0	32.0	36.0
13	34.98300073071017	36.0	36.0	36.0	36.0	36.0
14	34.91338945629751	36.0	36.0	36.0	32.0	36.0
15	34.892940920729735	36.0	36.0	36.0	32.0	36.0
16	34.89983683425276	36.0	36.0	36.0	32.0	36.0
17	34.84318977250208	36.0	36.0	36.0	32.0	36.0
18	34.84875609034267	36.0	36.0	36.0	32.0	36.0
19	34.84758014458807	36.0	36.0	36.0	32.0	36.0
20	34.83363815965799	36.0	36.0	36.0	32.0	36.0
21	34.82012918816093	36.0	36.0	36.0	32.0	36.0
22	34.80528363689379	36.0	36.0	36.0	32.0	36.0
23	34.73993003690217	36.0	36.0	36.0	32.0	36.0
24	34.71339399595447	36.0	36.0	36.0	32.0	36.0
25	34.678952840821296	36.0	36.0	36.0	32.0	36.0
26	34.62342934416798	36.0	36.0	36.0	32.0	36.0
27	34.61615803593488	36.0	36.0	36.0	32.0	36.0
28	34.57244375845184	36.0	36.0	36.0	32.0	36.0
29	34.52853130748232	36.0	36.0	36.0	32.0	36.0
30	34.501111779449495	36.0	36.0	36.0	32.0	36.0
31	34.50798586769868	36.0	36.0	36.0	32.0	36.0
32	34.4736259025842	36.0	36.0	36.0	32.0	36.0
33	34.43042320952001	36.0	36.0	36.0	32.0	36.0
34	34.41912819380145	36.0	36.0	36.0	32.0	36.0
35	34.380451189521075	36.0	36.0	36.0	32.0	36.0
36	34.33560810887494	36.0	36.0	36.0	32.0	36.0
37	34.35999654287662	36.0	36.0	36.0	32.0	36.0
38	33.89009752405364	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	23.0
22	71.0
23	273.0
24	732.0
25	1867.0
26	3938.0
27	7794.0
28	13700.0
29	21385.0
30	32379.0
31	46419.0
32	66476.0
33	107019.0
34	261270.0
35	582113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44384599064573	19.124920658064415	12.276936750835327	25.15429660045453
2	16.005608222366366	20.99949278063592	36.28975582756637	26.705143169431345
3	17.867653285445773	25.21203253537222	28.79652821003945	28.123785969142556
4	11.22544654549264	16.216541825991303	36.82066593333683	35.73734569517923
5	13.6366056984917	38.01910322568817	32.69548243021806	15.648808645602077
6	33.529936086644895	36.88073843016368	16.759075198807114	12.830250284384311
7	30.01361028829541	31.202218852388174	20.502646541947886	18.281524317368525
8	29.073707240118836	31.654643598905917	19.13514230813218	20.136506852843063
9	27.666090746307226	13.493910056896507	18.983196454030118	39.85680274276614
10	16.602877830993343	25.976693794382278	30.043930486465538	27.376497888158845
11	38.331971377984516	21.603318281980634	21.452892059800316	18.611818280234534
12	24.479772318544516	25.03142842176942	27.557837026172894	22.930962233513174
13	28.731102553648803	19.12927481472493	26.032882887995118	26.10673974363116
14	23.756548673416205	20.65072490464538	24.50157622127685	31.091150200661566
15	25.1354694747355	27.707272443147346	22.51346837648772	24.643789705629437
16	26.294391515730346	25.94003636963633	23.48897081611683	24.276601298516493
17	23.978380756743356	26.194344460439943	24.427632193501132	25.39964258931557
18	25.549538570060438	23.955071364280407	26.563540792746327	23.931849272912828
19	25.009406693025777	25.202254812691134	25.73147405280494	24.056864441478147
20	25.782719795785276	23.62498592269837	25.123421923574874	25.468872357941475
21	28.345992003212682	23.780489934174916	24.112059783842298	23.7614582787701
22	26.338740472176703	24.08087224270403	25.370134819081574	24.21025246603769
23	24.19440591064368	23.586352719739423	26.225469440634715	25.993771928982177
24	24.181093900185164	25.409333010901285	25.619554048544646	24.7900190403689
25	24.519319749271034	24.5204546645016	25.604211408517102	25.35601417771026
26	24.0939011401533	26.08218532292703	26.354303074747264	23.4696104621724
27	25.90232544739785	24.32723358449946	25.11111285713912	24.65932811096357
28	24.03547567311327	24.461766921789568	26.82526947665612	24.67748792844104
29	23.71350923340035	24.723757508985464	26.75787303103292	24.804860226581262
30	23.56588308113502	25.36341263473833	27.074776007214563	23.99592827691209
31	24.760773173421008	25.230191163208527	24.798138042238016	25.21089762113245
32	24.62152792631089	24.7236702078901	24.85191551698399	25.80288634881502
33	23.80631029777531	24.31230744652153	25.79817208966521	26.08321016603795
34	25.27873057223249	24.813677637213313	26.025067636523637	23.88252415403056
35	26.18849528705037	23.862532203191552	26.24105054646121	23.707921963296876
36	23.54763715220335	25.58332409396741	25.49026112630635	25.37877762752289
37	26.267939283834195	25.13267583968376	24.808963378063503	23.79042149841854
38	23.78319627049395	24.335812686606253	26.078169469034272	25.80282157386552
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	42.0
2	59.0
3	59.0
4	273.5
5	488.0
6	488.0
7	737.5
8	987.0
9	1137.5
10	1288.0
11	1288.0
12	1699.5
13	2111.0
14	2818.0
15	3525.0
16	3525.0
17	5199.5
18	6874.0
19	6874.0
20	7881.5
21	8889.0
22	9826.5
23	10764.0
24	10764.0
25	12209.0
26	13654.0
27	13654.0
28	17429.5
29	21205.0
30	24592.5
31	27980.0
32	27980.0
33	35896.0
34	43812.0
35	43812.0
36	46526.0
37	49240.0
38	56986.0
39	64732.0
40	64732.0
41	67485.5
42	70239.0
43	82002.5
44	93766.0
45	93766.0
46	91815.0
47	89864.0
48	89864.0
49	94632.5
50	99401.0
51	100953.5
52	102506.0
53	102506.0
54	96767.0
55	91028.0
56	91028.0
57	88073.5
58	85119.0
59	74767.5
60	64416.0
61	64416.0
62	65173.0
63	65930.0
64	55784.0
65	45638.0
66	45638.0
67	39699.0
68	33760.0
69	33760.0
70	27114.5
71	20469.0
72	15193.0
73	9917.0
74	9917.0
75	7576.5
76	5236.0
77	5236.0
78	5106.5
79	4977.0
80	4086.5
81	3196.0
82	3196.0
83	2950.0
84	2704.0
85	2704.0
86	1797.0
87	890.0
88	722.0
89	554.0
90	554.0
91	346.5
92	139.0
93	94.0
94	49.0
95	49.0
96	30.5
97	12.0
98	12.0
99	15.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008904711727418043
2	0.0
3	0.0
4	8.730109536684357E-5
5	0.0
6	3.492043814673743E-4
7	3.492043814673743E-4
8	0.0020952262888042457
9	0.004190452577608491
10	0.0013095164305026534
11	0.004277753672975335
12	8.730109536684357E-5
13	1.7460219073368714E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	8.730109536684357E-5
22	0.0
23	1.7460219073368714E-4
24	0.0
25	8.730109536684357E-5
26	8.730109536684357E-5
27	3.492043814673743E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.730109536684357E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1145461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.16183396566951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53653884207554	31.092224701588595
2	15.011748536465245	12.358222015847293
3	4.569961235474317	5.643239568124192
4	1.857664720716087	3.058595471919896
5	0.8471111619623828	1.7434324499580485
6	0.47550925237046704	1.1743699737127684
7	0.27440372065723384	0.7906472272478512
8	0.17931067374291637	0.5904604944702607
9	0.1254853767268402	0.46486874177547166
>10	0.7819762894083891	6.469098240854065
>50	0.13816507141981824	4.11498116009922
>100	0.15990911317602835	14.300910790031867
>500	0.02814400386983526	8.165730449153873
>1k	0.01407200193491763	10.033218715216655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4191	0.3658788906824414	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3217	0.2808476237951358	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3161	0.27595876245459255	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3044	0.2657445342966718	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2893	0.25256206889627847	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2517	0.21973685703834525	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2509	0.2190384482754105	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2466	0.21528450117463627	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2398	0.20934802668969085	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2371	0.2069908971147861	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2299	0.20070521824837334	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2266	0.1978242821012675	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2244	0.19590365800319698	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2214	0.19328462514219164	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2168	0.18926877475531687	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2157	0.18830846270628157	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2099	0.18324499917500464	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	2058	0.17966565426496406	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1922	0.16779270529507334	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1842	0.16080861766572585	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1836	0.1602848110935248	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1825	0.1593244990444895	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1808	0.15784038042325316	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1787	0.15600705742054946	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1756	0.1533007234641773	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1728	0.1508562927939057	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1681	0.14675314131166403	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC	1667	0.14553092597652822	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC	1639	0.1430864953062566	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1619	0.14134047339891972	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	1614	0.14090396792208554	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1609	0.1404674624452513	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1594	0.13915794601474865	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1588	0.1386341394425476	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG	1575	0.13749922520277863	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1574	0.13741192410741176	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1545	0.13488019234177331	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	1500	0.13095164305026535	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	1497	0.13068973976416481	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1493	0.13034053538269746	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC	1492	0.1302532342873306	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1480	0.1292056211429285	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA	1429	0.12475326527921947	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1345	0.11741997326840459	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1333	0.11637236012400247	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1329	0.11602315574253509	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1302	0.11366602616763033	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1293	0.11288031630932874	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1269	0.11078509002052449	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1256	0.10965017578075552	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA	1211	0.10572162648924754	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1211	0.10572162648924754	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA	1206	0.10528512101241334	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA	1184	0.10336449691434278	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1164	0.10161847500700591	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGA	1157	0.10100736733943799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	8.730109536684357E-5
15	0.0	0.0	0.0	0.0	8.730109536684357E-5
16	0.0	0.0	0.0	0.0	8.730109536684357E-5
17	0.0	0.0	0.0	0.0	8.730109536684357E-5
18	0.0	0.0	0.0	8.730109536684357E-5	1.7460219073368714E-4
19	0.0	0.0	0.0	2.619032861005307E-4	1.7460219073368714E-4
20	0.0	0.0	0.0	2.619032861005307E-4	1.7460219073368714E-4
21	8.730109536684357E-5	0.0	0.0	3.492043814673743E-4	1.7460219073368714E-4
22	8.730109536684357E-5	0.0	0.0	5.238065722010614E-4	1.7460219073368714E-4
23	8.730109536684357E-5	0.0	0.0	0.0011349142397689663	1.7460219073368714E-4
24	8.730109536684357E-5	0.0	0.0	0.002182527384171089	1.7460219073368714E-4
25	8.730109536684357E-5	0.0	0.0	0.0023571295749047765	1.7460219073368714E-4
26	8.730109536684357E-5	0.0	0.0	0.002968237242472681	1.7460219073368714E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	95	7.2759576E-11	21.89614	4
TCTAGCG	560	0.0	19.428122	28
TAGCGGC	575	0.0	18.921299	30
CTAGCGG	575	0.0	18.643045	29
AGGCCCG	465	0.0	17.892057	10
CGTCTCG	45	0.008851438	17.777365	10
AGCGGCG	630	0.0	17.523403	31
CATCGTT	515	0.0	17.397655	28
CAAGACG	630	0.0	17.270948	4
ATAATAC	75	3.2318967E-5	17.067762	3
CGGCATC	535	0.0	17.046333	25
GCATCGT	540	0.0	16.888498	27
GACTGTG	165	1.8189894E-12	16.485907	7
GCGGCGC	655	0.0	16.366034	32
AAGACGG	665	0.0	16.361952	5
CGTTTAT	555	0.0	16.14377	31
CGCAAGA	615	0.0	16.130411	2
GCGCAAG	630	0.0	16.002422	1
GGCATCG	585	0.0	15.86288	26
ATCGTTT	565	0.0	15.85804	29
>>END_MODULE