FastQCFastQC Report
Thu 2 Feb 2017
SRR4062864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377165
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29650.21529736814397696No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23940.17383537920292774No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23220.16860724749757652No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20800.1510349159323683No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20090.1458793971673692No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19710.1431201054339894No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19400.1408691042830743No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19220.13956207135673648No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18940.1375289090268777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18740.13607665021983567No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18410.13368042318821638No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18100.13142942203730126No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16870.12249803037399296No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15930.11567241398089555No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC15550.11291312224751572No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15400.11182392814223423No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15290.11102518579836113No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14670.10652318349653092No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14280.10369127882279901No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14150.1027473105982217No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13980.101512890612236No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13920.1010772129701234No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT13810.1002784706262503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5750.021.14763529
TCTAGCG5900.020.88116628
ACTAGAC707.7981895E-720.5682543
TAGCGGC6000.020.26574730
CAAGACG5300.019.0138634
CGCTTCG3200.018.49915932
AAGACGG5450.018.4905455
AGCGGCG6600.018.42340531
CGGTCCA7050.018.3834810
AGCGCGT1055.982656E-918.28488332
GCGCAAG5550.017.8893031
CTAGACA1101.0672011E-817.4499514
CGCCGGT7250.017.4317917
ACGCTTC3400.017.41097631
CGCAAGA5750.017.2557772
ATCGTTT4000.017.19984229
AGGCCCG4800.017.00046310
GCCGGTC7800.016.8185278
GTCCTAA3350.016.730851
TAGGACG4400.016.722874
Produced by FastQC (version 0.11.5)