##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062864_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1377165
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244962658795423	32.0	32.0	32.0	32.0	32.0
2	30.821291566370043	32.0	32.0	32.0	32.0	32.0
3	30.883430090076352	32.0	32.0	32.0	32.0	32.0
4	30.95274059390124	32.0	32.0	32.0	32.0	32.0
5	30.837651987960776	32.0	32.0	32.0	32.0	32.0
6	34.52332073498818	36.0	36.0	36.0	32.0	36.0
7	34.4462573475219	36.0	36.0	36.0	32.0	36.0
8	34.415399752389874	36.0	36.0	36.0	32.0	36.0
9	34.58580417016116	36.0	36.0	36.0	32.0	36.0
10	34.25783838537866	36.0	36.0	36.0	32.0	36.0
11	34.56874884273126	36.0	36.0	36.0	32.0	36.0
12	34.35698482026482	36.0	36.0	36.0	32.0	36.0
13	34.465072086496534	36.0	36.0	36.0	32.0	36.0
14	34.36174895528132	36.0	36.0	36.0	32.0	36.0
15	34.292986679156094	36.0	36.0	36.0	32.0	36.0
16	34.30797035939775	36.0	36.0	36.0	32.0	36.0
17	34.231613495841096	36.0	36.0	36.0	32.0	36.0
18	34.261291856821806	36.0	36.0	36.0	32.0	36.0
19	34.24771614149358	36.0	36.0	36.0	32.0	36.0
20	34.22717176227975	36.0	36.0	36.0	32.0	36.0
21	34.214009214582134	36.0	36.0	36.0	32.0	36.0
22	34.17828219567009	36.0	36.0	36.0	32.0	36.0
23	34.10956276117967	36.0	36.0	36.0	32.0	36.0
24	34.09720331260234	36.0	36.0	36.0	32.0	36.0
25	34.063576986054684	36.0	36.0	36.0	32.0	36.0
26	34.02237640369891	36.0	36.0	36.0	32.0	36.0
27	34.01837252616789	36.0	36.0	36.0	32.0	36.0
28	34.001270000326755	36.0	36.0	36.0	32.0	36.0
29	33.96530989387619	36.0	36.0	36.0	32.0	36.0
30	33.917948829660936	36.0	36.0	36.0	32.0	36.0
31	33.95218655716635	36.0	36.0	36.0	32.0	36.0
32	33.89586868675867	36.0	36.0	36.0	32.0	36.0
33	33.86361111413665	36.0	36.0	36.0	32.0	36.0
34	33.87465699462301	36.0	36.0	36.0	32.0	36.0
35	33.829565810923164	36.0	36.0	36.0	32.0	36.0
36	33.78594068248903	36.0	36.0	36.0	32.0	36.0
37	33.775121354376566	36.0	36.0	36.0	32.0	36.0
38	33.15616647242705	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	5.0
10	10.0
11	14.0
12	7.0
13	7.0
14	120.0
15	279.0
16	433.0
17	484.0
18	651.0
19	834.0
20	1152.0
21	1711.0
22	2596.0
23	3997.0
24	6172.0
25	9303.0
26	13687.0
27	19689.0
28	27810.0
29	37606.0
30	50649.0
31	68108.0
32	92572.0
33	138727.0
34	302831.0
35	597704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9627085349283	17.047900782921566	11.04036455398771	25.949026128162423
2	16.776698408408475	19.497304967012276	37.73300465482581	25.992991969753444
3	19.12076978939724	23.652505446623092	28.40312273057371	28.823602033405955
4	12.24119163854657	14.858520879307601	36.437036762714314	36.463250719431514
5	14.371770993308717	36.744834496955704	33.84873998395254	15.03465452578304
6	34.53863553023784	35.85038829769853	16.620375917192202	12.99060025487142
7	30.34240632023033	30.30791517356308	20.82640787414725	18.523270632059337
8	28.287002444888355	33.11193551361898	19.476400328502375	19.124661712990292
9	27.53633922516015	13.719518836563891	18.546982853142914	40.19715908513305
10	15.653698162228416	26.40446949766802	31.647662539565207	26.294169800538352
11	38.482160881012994	20.85122667088797	21.888084162707987	18.77852828539105
12	24.62683207370702	23.63890647782922	28.95678542590086	22.777476022562897
13	29.718443852170314	18.938263894436417	25.45094735618995	25.89234489720332
14	23.34933372063469	19.82208343923605	24.89720779928107	31.93137504084819
15	25.25163757797515	27.54479145256562	22.097697622379858	25.105873347079378
16	25.972782001844553	25.597844636645533	24.114144208913384	24.31522915259653
17	23.98109104853317	25.926891585095362	25.362440936761484	24.729576429609985
18	25.11927290366525	24.46993451427426	26.746177094664564	23.66461548739592
19	25.757211564646106	24.770688837587933	25.2841018431309	24.18799775463506
20	25.65326063908584	24.192428683050952	25.23215587629585	24.922154801567363
21	27.167088574503406	24.110907297916313	24.076196119609783	24.645808007970498
22	25.726541531597473	24.500316613800877	25.13063950178349	24.642502352818152
23	24.1097273156385	24.031661885915547	25.752804909044695	26.105805889401257
24	24.63819688131812	25.133479352095	25.30588413004578	24.9224396365411
25	24.945604810597256	24.500058099000697	25.233993725307926	25.32034336509412
26	24.17861344720903	25.682310049820618	26.058869667523638	24.080206835446717
27	25.40804440778008	24.70204552320312	25.12831722166709	24.761592847349707
28	24.013678158081632	24.890239244317346	25.944930091512074	25.15115250608894
29	24.08415212449983	24.96525130171456	25.818609616348226	25.131986957437384
30	24.230064307747718	25.371062094445833	25.99678461261408	24.40208898519237
31	25.059092212898985	25.096780518268663	24.60523497097129	25.23889229786106
32	24.664742297986674	25.009095365176186	24.79734219269103	25.52882014414611
33	24.146116877816684	24.53092162880103	25.61068419577332	25.712277297608964
34	25.081244694131428	24.984077855474133	25.72771644131174	24.206961009082704
35	25.919045557934368	24.586847081857872	25.57263328520841	23.921474074999345
36	24.15247805894763	25.707542202710997	25.119300500731672	25.020679237609706
37	25.54390174294012	25.298447903875047	24.883137103397793	24.274513249787045
38	24.55376593952421	24.927745664739884	25.44783164376797	25.070656751967935
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	93.0
2	129.0
3	129.0
4	330.0
5	531.0
6	531.0
7	707.0
8	883.0
9	923.0
10	963.0
11	963.0
12	1255.0
13	1547.0
14	2140.5
15	2734.0
16	2734.0
17	3934.0
18	5134.0
19	5134.0
20	6432.5
21	7731.0
22	8647.5
23	9564.0
24	9564.0
25	12298.0
26	15032.0
27	15032.0
28	19652.0
29	24272.0
30	29682.0
31	35092.0
32	35092.0
33	44048.0
34	53004.0
35	53004.0
36	58787.5
37	64571.0
38	73510.0
39	82449.0
40	82449.0
41	84939.5
42	87430.0
43	97717.0
44	108004.0
45	108004.0
46	109937.5
47	111871.0
48	111871.0
49	116950.0
50	122029.0
51	123047.0
52	124065.0
53	124065.0
54	116278.5
55	108492.0
56	108492.0
57	105841.0
58	103190.0
59	92362.5
60	81535.0
61	81535.0
62	79148.0
63	76761.0
64	64427.5
65	52094.0
66	52094.0
67	44437.5
68	36781.0
69	36781.0
70	29850.0
71	22919.0
72	18176.0
73	13433.0
74	13433.0
75	10348.0
76	7263.0
77	7263.0
78	6792.5
79	6322.0
80	5055.5
81	3789.0
82	3789.0
83	3727.5
84	3666.0
85	3666.0
86	2480.5
87	1295.0
88	1075.0
89	855.0
90	855.0
91	628.5
92	402.0
93	351.0
94	300.0
95	300.0
96	280.0
97	260.0
98	260.0
99	488.0
100	716.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12169928803011985
2	0.054024027621962505
3	0.011981135158096526
4	0.0026140658526756055
5	0.0
6	0.0
7	0.0
8	4.356776421126009E-4
9	2.1783882105630046E-4
10	5.809035228168012E-4
11	0.0016700976280983033
12	0.0026866787930277056
13	0.012707264561617526
14	0.008350488140491516
15	0.020622075059996442
16	0.00980274694753352
17	0.018225848028377137
18	0.0051555187649991105
19	0.007696971677322616
20	0.005300744645703311
21	0.006317325810632712
22	0.006753003452745313
23	0.008350488140491516
24	0.012562038680913327
25	0.01488565277218053
26	0.016337911579222535
27	0.007914810498378918
28	0.005736422287815912
29	0.00980274694753352
30	0.0026866787930277056
31	0.005809035228168012
32	0.006535164631689013
33	0.007479132856266316
34	0.011037166933519223
35	0.014014297487955327
36	0.013070329263378026
37	0.009004004603660418
38	0.006172099929928512
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1377165.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.48028474061087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14149674473616	39.44605005731799
2	13.043532164685859	13.168824353933173
3	4.047350901768594	6.129342778993404
4	1.6632180838773485	3.35838889839447
5	0.847504532580388	2.1391135061808155
6	0.4854929152329086	1.470469236030389
7	0.3043609976609352	1.0754960878102287
8	0.21297688852862984	0.8600907180875661
9	0.15349844221116166	0.6973780563053679
>10	0.8901852538326853	8.427193043235016
>50	0.09286207256417958	3.2936844850917266
>100	0.09798243166125907	10.683233215906744
>500	0.013459904232490641	4.620180507628153
>1k	0.006078666427576419	4.630555055085113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2965	0.21529736814397696	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2394	0.17383537920292774	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2322	0.16860724749757652	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2080	0.1510349159323683	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2009	0.1458793971673692	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1971	0.1431201054339894	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1940	0.1408691042830743	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1922	0.13956207135673648	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1894	0.1375289090268777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1874	0.13607665021983567	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1841	0.13368042318821638	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1810	0.13142942203730126	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1687	0.12249803037399296	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1593	0.11567241398089555	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1555	0.11291312224751572	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1540	0.11182392814223423	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1529	0.11102518579836113	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1467	0.10652318349653092	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1428	0.10369127882279901	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1415	0.1027473105982217	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1398	0.101512890612236	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1392	0.1010772129701234	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1381	0.1002784706262503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.261294035210014E-5
6	0.0	0.0	0.0	0.0	7.261294035210014E-5
7	0.0	0.0	0.0	0.0	7.261294035210014E-5
8	0.0	0.0	0.0	0.0	7.261294035210014E-5
9	0.0	0.0	0.0	0.0	7.261294035210014E-5
10	0.0	0.0	0.0	7.261294035210014E-5	1.452258807042003E-4
11	0.0	0.0	0.0	7.261294035210014E-5	1.452258807042003E-4
12	0.0	0.0	0.0	7.261294035210014E-5	2.1783882105630043E-4
13	0.0	0.0	0.0	7.261294035210014E-5	2.1783882105630043E-4
14	0.0	0.0	0.0	7.261294035210014E-5	2.1783882105630043E-4
15	0.0	0.0	0.0	1.452258807042003E-4	2.1783882105630043E-4
16	0.0	0.0	0.0	1.452258807042003E-4	2.1783882105630043E-4
17	0.0	0.0	0.0	1.452258807042003E-4	2.1783882105630043E-4
18	0.0	0.0	0.0	1.452258807042003E-4	2.1783882105630043E-4
19	0.0	0.0	0.0	1.452258807042003E-4	2.1783882105630043E-4
20	0.0	0.0	0.0	2.904517614084006E-4	2.904517614084006E-4
21	0.0	0.0	0.0	3.6306470176050075E-4	2.904517614084006E-4
22	0.0	0.0	0.0	5.809035228168012E-4	2.904517614084006E-4
23	0.0	0.0	0.0	0.0011618070456336023	2.904517614084006E-4
24	0.0	0.0	0.0	0.001960549389506704	2.904517614084006E-4
25	0.0	0.0	0.0	0.0023962270316193047	2.904517614084006E-4
26	0.0	0.0	0.0	0.002904517614084006	2.904517614084006E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	575	0.0	21.147635	29
TCTAGCG	590	0.0	20.881166	28
ACTAGAC	70	7.7981895E-7	20.568254	3
TAGCGGC	600	0.0	20.265747	30
CAAGACG	530	0.0	19.013863	4
CGCTTCG	320	0.0	18.499159	32
AAGACGG	545	0.0	18.490545	5
AGCGGCG	660	0.0	18.423405	31
CGGTCCA	705	0.0	18.38348	10
AGCGCGT	105	5.982656E-9	18.284883	32
GCGCAAG	555	0.0	17.889303	1
CTAGACA	110	1.0672011E-8	17.449951	4
CGCCGGT	725	0.0	17.431791	7
ACGCTTC	340	0.0	17.410976	31
CGCAAGA	575	0.0	17.255777	2
ATCGTTT	400	0.0	17.199842	29
AGGCCCG	480	0.0	17.000463	10
GCCGGTC	780	0.0	16.818527	8
GTCCTAA	335	0.0	16.73085	1
TAGGACG	440	0.0	16.72287	4
>>END_MODULE