FastQCFastQC Report
Thu 2 Feb 2017
SRR4062864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377165
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29780.21624133636855425No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27200.1975071977577124No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23390.16984166748356225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23190.16838940867652025No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21650.15720701586229682No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20550.1492195924235658No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20450.14849346302004482No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20360.14783994655687588No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20290.1473316559744112No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18720.1359314243391315No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18070.13121158321624496No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17640.12808922678110465No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16180.11748773748969804No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16030.11639854338441655No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16020.11632593044406445No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15300.11109779873871323No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15220.11051689521589642No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15200.11037166933519224No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14570.10579705409300992No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14510.10536137645089731No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14410.10463524704737633No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14330.10405434352455951No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14200.1031103752999822No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT14120.10252947177716541No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14020.10180334237364441No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14010.10173072943329231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG601.13747905E-418.6686151
GCGCAAG5100.017.884221
CAAGACG5050.017.743144
CGCAAGA5050.017.7424952
AAGACGG5100.017.5704615
CTACTAT1101.0622898E-817.4563671
TCTAGCG4500.017.4215128
TCTAGAT3500.017.371352
TAGCGGC4650.017.20359830
CTAGCGG4700.017.02058229
ATCGTTT3900.016.40958829
GTTTTCG5500.016.29024528
TCGTTTA3950.016.2018730
TAGACAG2100.016.001675
TATGTCG703.6941262E-416.001675
GATATAC3100.016.0016691
CATCGTT4000.015.99934628
TTTTCGG5650.015.85775929
AGCGGCG5200.015.69166731
CGGCATC4200.015.61841125
Produced by FastQC (version 0.11.5)