##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1377165
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20473944661678	32.0	32.0	32.0	32.0	32.0
2	31.28207658486819	32.0	32.0	32.0	32.0	32.0
3	31.384284381319596	32.0	32.0	32.0	32.0	32.0
4	31.48317812317333	32.0	32.0	32.0	32.0	32.0
5	31.395287420171147	32.0	32.0	32.0	32.0	32.0
6	34.93165016537597	36.0	36.0	36.0	36.0	36.0
7	34.966921901152006	36.0	36.0	36.0	36.0	36.0
8	34.90158550355259	36.0	36.0	36.0	36.0	36.0
9	35.023397341640255	36.0	36.0	36.0	36.0	36.0
10	34.859135252493346	36.0	36.0	36.0	32.0	36.0
11	35.04278281832605	36.0	36.0	36.0	36.0	36.0
12	34.92467351406694	36.0	36.0	36.0	32.0	36.0
13	34.99071716170539	36.0	36.0	36.0	36.0	36.0
14	34.934198153452925	36.0	36.0	36.0	32.0	36.0
15	34.90712224025444	36.0	36.0	36.0	32.0	36.0
16	34.91122487138433	36.0	36.0	36.0	32.0	36.0
17	34.88045295952192	36.0	36.0	36.0	32.0	36.0
18	34.88332843195986	36.0	36.0	36.0	32.0	36.0
19	34.881372965476174	36.0	36.0	36.0	32.0	36.0
20	34.85812956326947	36.0	36.0	36.0	32.0	36.0
21	34.84956196243733	36.0	36.0	36.0	32.0	36.0
22	34.823424934557586	36.0	36.0	36.0	32.0	36.0
23	34.76485315848137	36.0	36.0	36.0	32.0	36.0
24	34.74406262139976	36.0	36.0	36.0	32.0	36.0
25	34.71121325331387	36.0	36.0	36.0	32.0	36.0
26	34.652486811674706	36.0	36.0	36.0	32.0	36.0
27	34.63289075746189	36.0	36.0	36.0	32.0	36.0
28	34.599425631641815	36.0	36.0	36.0	32.0	36.0
29	34.56658352484996	36.0	36.0	36.0	32.0	36.0
30	34.530005482277	36.0	36.0	36.0	32.0	36.0
31	34.533577312812916	36.0	36.0	36.0	32.0	36.0
32	34.50694651693878	36.0	36.0	36.0	32.0	36.0
33	34.47057324285761	36.0	36.0	36.0	32.0	36.0
34	34.46357045089005	36.0	36.0	36.0	32.0	36.0
35	34.423305123206006	36.0	36.0	36.0	32.0	36.0
36	34.3965363627452	36.0	36.0	36.0	32.0	36.0
37	34.38241314584672	36.0	36.0	36.0	32.0	36.0
38	33.928455922129885	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	23.0
22	103.0
23	314.0
24	863.0
25	2061.0
26	4402.0
27	9007.0
28	15465.0
29	24628.0
30	38002.0
31	54930.0
32	79697.0
33	129856.0
34	317177.0
35	700631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.243527831233436	18.245742710867436	11.744381104535057	26.766348353364076
2	15.687299633667717	20.466175077060484	37.047412619402905	26.799112669868897
3	18.19781943340123	24.216342994485046	28.781591167361935	28.804246404751794
4	11.773949122795552	15.683156411133355	36.447425460676655	36.095469005394435
5	13.851156326447922	37.31504549570385	33.52094123934494	15.312856938503286
6	34.61674471358873	36.285392506188444	16.20164061915692	12.896222161065902
7	30.075038376022572	30.505125758625347	20.77032331708252	18.649512548269556
8	28.135018462108203	33.55434612912947	19.16595565463332	19.14467975412901
9	27.33079324414701	13.509309666498925	18.30033323239783	40.859563856956235
10	15.40881256685016	26.728446563801832	31.592415334020263	26.27032553532775
11	38.59055699620139	20.648283364183566	21.838988644838402	18.92217099477664
12	24.28940924973351	23.844654667127518	28.88711874547984	22.978817337659134
13	29.6972029393825	18.904266751866157	25.461602137732726	25.936928171018618
14	23.260265298831513	19.801635825508075	24.79552181149086	32.14257706416955
15	25.18521745760312	27.71679501003874	21.979210915177195	25.11877661718095
16	25.930718390429014	25.641355192780512	23.967768497823787	24.460157918966686
17	23.930828913020587	26.02999640565945	25.27300650248881	24.76616817883115
18	25.18870287874002	24.52298744159197	26.640090330497795	23.648219349170216
19	25.682616099015004	24.85025396375888	25.186742329350515	24.2803876078756
20	25.6738299332324	24.15970490101041	25.13141126880221	25.035053896954977
21	27.116668748239135	24.13089508584308	24.121310170756715	24.63112599516107
22	25.623527771565335	24.52532886424565	25.095994376849816	24.755148987339197
23	23.90980588354465	24.033901578825457	25.777849099924993	26.278443437704908
24	24.652601540120465	25.10178518913856	25.265236917871132	24.980376352869847
25	24.840559904673594	24.46616704050283	25.344131377331514	25.34914167749206
26	24.201637714635087	25.70487298889093	25.946238753146865	24.147250543327115
27	25.479245754318296	24.706788733948898	24.98024926588264	24.83371624585017
28	24.07017314555627	24.835440923927052	26.051852900705434	25.042533029811242
29	24.060443011549086	24.747288814339605	25.971034698093547	25.221233476017762
30	24.14002679417499	25.234085966460086	26.104352056580005	24.521535182784923
31	25.050520453249973	24.90071995730359	24.714467765300455	25.334291824145982
32	24.699364273707218	24.987201969262944	24.84306528266402	25.470368474365817
33	24.127609981374782	24.401215540621493	25.752614973514433	25.718559504489296
34	25.032221992281244	24.922866904110982	25.743756194791473	24.3011549088163
35	25.898494370681803	24.557623814139916	25.51952743498419	24.024354380194094
36	24.105680873388447	25.599909959953965	25.19305965516115	25.101349511496444
37	25.62641368318248	25.189138556382133	24.865212229471414	24.319235530963972
38	24.556642895575905	24.74877701477603	25.4968366126595	25.19774347698856
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	44.0
2	73.0
3	73.0
4	259.0
5	445.0
6	445.0
7	646.0
8	847.0
9	882.0
10	917.0
11	917.0
12	1230.0
13	1543.0
14	2152.0
15	2761.0
16	2761.0
17	4120.5
18	5480.0
19	5480.0
20	6482.5
21	7485.0
22	8646.0
23	9807.0
24	9807.0
25	12317.5
26	14828.0
27	14828.0
28	19658.0
29	24488.0
30	29727.0
31	34966.0
32	34966.0
33	44169.0
34	53372.0
35	53372.0
36	58835.0
37	64298.0
38	73488.5
39	82679.0
40	82679.0
41	85051.0
42	87423.0
43	97585.0
44	107747.0
45	107747.0
46	109582.0
47	111417.0
48	111417.0
49	117739.5
50	124062.0
51	123868.5
52	123675.0
53	123675.0
54	116430.5
55	109186.0
56	109186.0
57	106600.5
58	104015.0
59	92497.0
60	80979.0
61	80979.0
62	79034.0
63	77089.0
64	64765.0
65	52441.0
66	52441.0
67	44507.5
68	36574.0
69	36574.0
70	29934.0
71	23294.0
72	18440.5
73	13587.0
74	13587.0
75	10271.5
76	6956.0
77	6956.0
78	6622.0
79	6288.0
80	4933.0
81	3578.0
82	3578.0
83	3406.5
84	3235.0
85	3235.0
86	2039.0
87	843.0
88	700.5
89	558.0
90	558.0
91	342.0
92	126.0
93	86.0
94	46.0
95	46.0
96	32.5
97	19.0
98	19.0
99	21.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008350488140491516
2	0.0
3	0.0
4	4.356776421126009E-4
5	1.452258807042003E-4
6	1.452258807042003E-4
7	2.1783882105630046E-4
8	0.001452258807042003
9	0.0034854211369008074
10	0.0013070329263378028
11	0.005954261108872212
12	7.261294035210014E-5
13	3.6306470176050075E-4
14	7.261294035210014E-5
15	0.0
16	2.1783882105630046E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	7.261294035210014E-5
22	7.261294035210014E-5
23	1.452258807042003E-4
24	0.0
25	1.452258807042003E-4
26	1.452258807042003E-4
27	7.261294035210014E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.452258807042003E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1377165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.23772749419732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35654111480936	36.83266022695648
2	14.128007460928933	13.630059478725528
3	4.32180801899298	6.254225925072604
4	1.8166687701288833	3.505278923227827
5	0.8931942799827894	2.1542831138592797
6	0.5304995271561606	1.5354054976055642
7	0.34993085486074094	1.1815908453008754
8	0.23978387258212988	0.925330328249609
9	0.172846243196125	0.7503938979921392
>10	0.9617843130435524	8.64657140707293
>50	0.1014745624144196	3.437931651657907
>100	0.10593964276700747	11.133622978664219
>500	0.01424652027369832	4.7231005903913434
>1k	0.007274818863165099	5.28954513522374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2978	0.21624133636855425	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2720	0.1975071977577124	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2339	0.16984166748356225	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2319	0.16838940867652025	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2165	0.15720701586229682	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2055	0.1492195924235658	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2045	0.14849346302004482	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2036	0.14783994655687588	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2029	0.1473316559744112	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1872	0.1359314243391315	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1807	0.13121158321624496	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1764	0.12808922678110465	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1618	0.11748773748969804	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1603	0.11639854338441655	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1602	0.11632593044406445	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1530	0.11109779873871323	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1522	0.11051689521589642	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1520	0.11037166933519224	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1457	0.10579705409300992	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1451	0.10536137645089731	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1441	0.10463524704737633	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1433	0.10405434352455951	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1420	0.1031103752999822	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1412	0.10252947177716541	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1402	0.10180334237364441	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1401	0.10173072943329231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.452258807042003E-4
6	0.0	0.0	0.0	0.0	2.1783882105630043E-4
7	0.0	0.0	0.0	0.0	2.1783882105630043E-4
8	0.0	0.0	0.0	0.0	2.1783882105630043E-4
9	0.0	0.0	0.0	0.0	2.1783882105630043E-4
10	0.0	0.0	0.0	0.0	2.1783882105630043E-4
11	0.0	0.0	0.0	0.0	2.1783882105630043E-4
12	0.0	0.0	0.0	0.0	2.904517614084006E-4
13	0.0	0.0	0.0	0.0	2.904517614084006E-4
14	0.0	0.0	0.0	0.0	2.904517614084006E-4
15	0.0	0.0	0.0	7.261294035210014E-5	2.904517614084006E-4
16	0.0	0.0	0.0	7.261294035210014E-5	2.904517614084006E-4
17	0.0	0.0	0.0	7.261294035210014E-5	2.904517614084006E-4
18	0.0	0.0	0.0	7.261294035210014E-5	2.904517614084006E-4
19	0.0	0.0	0.0	7.261294035210014E-5	2.904517614084006E-4
20	0.0	0.0	0.0	2.1783882105630043E-4	2.904517614084006E-4
21	0.0	0.0	0.0	2.904517614084006E-4	2.904517614084006E-4
22	0.0	0.0	0.0	5.08290582464701E-4	2.904517614084006E-4
23	0.0	0.0	0.0	0.001016581164929402	2.904517614084006E-4
24	0.0	0.0	0.0	0.0017427105684504035	2.904517614084006E-4
25	0.0	0.0	0.0	0.001887936449154604	2.904517614084006E-4
26	0.0	0.0	0.0	0.0023962270316193047	2.904517614084006E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACG	60	1.13747905E-4	18.668615	1
GCGCAAG	510	0.0	17.88422	1
CAAGACG	505	0.0	17.74314	4
CGCAAGA	505	0.0	17.742495	2
AAGACGG	510	0.0	17.570461	5
CTACTAT	110	1.0622898E-8	17.456367	1
TCTAGCG	450	0.0	17.42151	28
TCTAGAT	350	0.0	17.37135	2
TAGCGGC	465	0.0	17.203598	30
CTAGCGG	470	0.0	17.020582	29
ATCGTTT	390	0.0	16.409588	29
GTTTTCG	550	0.0	16.290245	28
TCGTTTA	395	0.0	16.20187	30
TAGACAG	210	0.0	16.00167	5
TATGTCG	70	3.6941262E-4	16.00167	5
GATATAC	310	0.0	16.001669	1
CATCGTT	400	0.0	15.999346	28
TTTTCGG	565	0.0	15.857759	29
AGCGGCG	520	0.0	15.691667	31
CGGCATC	420	0.0	15.618411	25
>>END_MODULE