FastQCFastQC Report
Thu 2 Feb 2017
SRR4062863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657293
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58420.3525025448125346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT41790.25215818808140744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24640.14867618459741277No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24420.1473487186635073No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24240.1462626101721301No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22150.13365168380002812No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21940.1323845572267547No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20940.12635062116354803No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20670.12472145842648222No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20310.12254924144372782No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20170.12170449039487886No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20100.12128211487045441No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19400.11705835962620972No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18750.11313630118512537No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18250.11011933315352204No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT18020.10873152785898449No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17290.10432675453284361No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16960.10233555563198542No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16770.10118910777997614No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16600.10016333864923099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA555.83651E-520.36308132
CTAGCGG4100.016.7800329
ACCGTCG4400.016.7247968
TAGGACG7500.016.6370354
TCTAGCG4200.016.37951528
ATACCGT4500.016.3521486
GTCCTAC9050.016.2819461
ATCGTTT4550.016.17538329
TAGCGGC4400.015.99956330
TACCGTC4600.015.9976327
TTAGGAC5300.015.9971493
CCGTCGT4650.015.8284819
CATCGTT4650.015.82656928
GACGTGA4650.015.8256157
AGGACGT8000.015.5967485
TGTAGGA8800.015.4583212
TCCTACA10600.015.3999882
CCTACAG10650.015.3212133
CTAGACA2100.015.2353794
GGACGTG8200.015.2163416
Produced by FastQC (version 0.11.5)