##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062863_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657293
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168752296666913	32.0	32.0	32.0	32.0	32.0
2	30.75509580985378	32.0	32.0	32.0	32.0	32.0
3	30.815794189681608	32.0	32.0	32.0	32.0	32.0
4	30.877826069379402	32.0	32.0	32.0	32.0	32.0
5	30.78785887588978	32.0	32.0	32.0	32.0	32.0
6	34.44328975021315	36.0	36.0	36.0	32.0	36.0
7	34.34254956727627	36.0	36.0	36.0	32.0	36.0
8	34.31867931621023	36.0	36.0	36.0	32.0	36.0
9	34.471874918918985	36.0	36.0	36.0	32.0	36.0
10	34.16678281993588	36.0	36.0	36.0	32.0	36.0
11	34.479484315688296	36.0	36.0	36.0	32.0	36.0
12	34.27565011135629	36.0	36.0	36.0	32.0	36.0
13	34.37536392176881	36.0	36.0	36.0	32.0	36.0
14	34.26757851508454	36.0	36.0	36.0	32.0	36.0
15	34.20279214357389	36.0	36.0	36.0	32.0	36.0
16	34.22925638375351	36.0	36.0	36.0	32.0	36.0
17	34.14729562002615	36.0	36.0	36.0	32.0	36.0
18	34.17647935518946	36.0	36.0	36.0	32.0	36.0
19	34.16396436840076	36.0	36.0	36.0	32.0	36.0
20	34.135741839252326	36.0	36.0	36.0	32.0	36.0
21	34.11849503980286	36.0	36.0	36.0	32.0	36.0
22	34.08472672001873	36.0	36.0	36.0	32.0	36.0
23	34.04332245414661	36.0	36.0	36.0	32.0	36.0
24	34.015951313376696	36.0	36.0	36.0	32.0	36.0
25	34.00910822648741	36.0	36.0	36.0	32.0	36.0
26	33.96104792574397	36.0	36.0	36.0	32.0	36.0
27	33.96445227247083	36.0	36.0	36.0	32.0	36.0
28	33.934065973850124	36.0	36.0	36.0	32.0	36.0
29	33.89480073831242	36.0	36.0	36.0	32.0	36.0
30	33.86113499544136	36.0	36.0	36.0	32.0	36.0
31	33.88508248088902	36.0	36.0	36.0	32.0	36.0
32	33.831831184950396	36.0	36.0	36.0	32.0	36.0
33	33.79235958879933	36.0	36.0	36.0	32.0	36.0
34	33.79398814814279	36.0	36.0	36.0	32.0	36.0
35	33.750201080919304	36.0	36.0	36.0	32.0	36.0
36	33.700260002304965	36.0	36.0	36.0	27.0	36.0
37	33.70040421337688	36.0	36.0	36.0	27.0	36.0
38	33.085065223831876	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	5.0
9	7.0
10	14.0
11	14.0
12	6.0
13	12.0
14	175.0
15	367.0
16	555.0
17	656.0
18	882.0
19	1167.0
20	1665.0
21	2335.0
22	3525.0
23	5512.0
24	8376.0
25	12347.0
26	18195.0
27	25478.0
28	35559.0
29	47231.0
30	63142.0
31	84302.0
32	112911.0
33	167605.0
34	362077.0
35	703168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.85667113106295	17.733983600744025	11.808600034192805	25.600745234000215
2	16.061330337290293	20.11009371489012	38.61581121065053	25.212764737169064
3	18.312873050902382	24.75703226032262	29.05070444803704	27.879390240737955
4	12.13031265745258	15.770269332536833	36.662774665182276	35.43664334482832
5	14.273620517375374	37.042384908356304	33.56525842219337	15.118736152074957
6	33.24127552658192	36.307965041766934	17.180134822348748	13.270624609302404
7	29.51517927125741	30.981667092059155	21.27891688434091	18.224236752342524
8	27.833179768548604	33.402482741309406	19.743242093525478	19.021095396616506
9	27.997306442815006	13.857036314750362	18.897318993439885	39.24833824899474
10	16.255862772653245	26.56004209309356	31.483534154478516	25.70056097977468
11	37.19580850825036	21.063476987947592	22.918102267474907	18.822612236327146
12	25.027274197402434	23.974683269331493	28.958982503481685	22.039060029784387
13	29.815748325163078	19.541866245993578	25.389688882558943	25.252696546284398
14	23.60997628429876	20.447218987043865	25.234230265579647	30.70857446307773
15	24.840951928584232	27.98328675004783	22.613343440628793	24.562417880739144
16	25.18965487067985	25.73832787496628	24.763244460617383	24.308772793736484
17	23.530629463816904	26.1632026381256	25.76572150731411	24.540446390743384
18	24.700533852603098	24.439490972417918	27.473164055735012	23.386811119243976
19	25.703973776886297	24.610901516084105	25.91867793410912	23.76644677292048
20	25.910942900727985	24.055023461486474	25.527340355687723	24.506693282097824
21	27.221753893481328	23.94530993070813	24.488759003930138	24.344177171880403
22	25.957740826899112	24.594923226855663	25.155763944833165	24.29157200141206
23	23.908803555070644	24.359765038989188	26.190464696106392	25.540966709833775
24	24.745616443729652	25.172702008696202	25.920961711963166	24.160719835610983
25	24.582272934931318	24.619689885896403	26.030610686884696	24.76742649228758
26	24.03805475138218	25.728599181282103	26.58980398688941	23.643542080446306
27	25.03501533674767	25.056075832266732	25.369569454677993	24.539339376307606
28	23.81702356448871	25.162427882744176	26.156274721713835	24.86427383105328
29	24.076865609494995	25.007995770991464	25.926755116991707	24.988383502521835
30	23.791240354421458	25.280225628697423	26.523802282837206	24.404731734043914
31	25.006305855092055	24.8751802750439	25.014331488845578	25.104182381018468
32	24.3159837819017	25.346722497089903	25.045967169152544	25.291326551855853
33	23.809724959538674	24.853331466661196	26.179473284663075	25.157470289137052
34	24.79267673018827	25.032617734633455	26.128404645779245	24.046300889399024
35	25.43764412367675	24.912839311115228	26.01519495810795	23.634321607100073
36	23.84308348531171	25.714378823850325	25.636770277495813	24.805767413342153
37	25.03810823825886	25.431863194140202	25.169118507870873	24.360910059730063
38	24.16711220104442	25.482414164734678	25.6912621757606	24.659211458460305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	563.0
1	475.5
2	388.0
3	388.0
4	861.5
5	1335.0
6	1335.0
7	1586.5
8	1838.0
9	1801.5
10	1765.0
11	1765.0
12	2294.0
13	2823.0
14	3606.5
15	4390.0
16	4390.0
17	6711.0
18	9032.0
19	9032.0
20	10737.5
21	12443.0
22	13323.5
23	14204.0
24	14204.0
25	16592.0
26	18980.0
27	18980.0
28	24831.0
29	30682.0
30	38539.5
31	46397.0
32	46397.0
33	57890.5
34	69384.0
35	69384.0
36	75448.0
37	81512.0
38	90708.0
39	99904.0
40	99904.0
41	102641.0
42	105378.0
43	117070.5
44	128763.0
45	128763.0
46	129728.0
47	130693.0
48	130693.0
49	138253.5
50	145814.0
51	145502.5
52	145191.0
53	145191.0
54	137775.0
55	130359.0
56	130359.0
57	126611.0
58	122863.0
59	110153.5
60	97444.0
61	97444.0
62	93871.0
63	90298.0
64	74818.0
65	59338.0
66	59338.0
67	50047.0
68	40756.0
69	40756.0
70	32751.0
71	24746.0
72	19281.0
73	13816.0
74	13816.0
75	10483.5
76	7151.0
77	7151.0
78	6996.5
79	6842.0
80	5318.5
81	3795.0
82	3795.0
83	3969.0
84	4143.0
85	4143.0
86	2772.5
87	1402.0
88	1170.0
89	938.0
90	938.0
91	712.5
92	487.0
93	420.5
94	354.0
95	354.0
96	321.5
97	289.0
98	289.0
99	541.0
100	793.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11910989788770002
2	0.05346067352001124
3	0.010861084913772035
4	0.00289628931033921
5	3.0169680316033436E-4
6	6.0339360632066865E-5
7	0.0
8	7.240723275848025E-4
9	3.0169680316033436E-4
10	8.447510488489361E-4
11	0.001870520179594073
12	0.0029566286709712766
13	0.016050269928129787
14	0.0098353157830269
15	0.024377101695355015
16	0.012309229568941642
17	0.021661830466912006
18	0.0056718998994142854
19	0.007783777521536626
20	0.006576990308895289
21	0.0060339360632066874
22	0.008326831767225228
23	0.010076673225555168
24	0.014964161436752584
25	0.017498414583299392
26	0.018825880517204865
27	0.009774976422394832
28	0.00579257862067842
29	0.010076673225555168
30	0.003439343556027812
31	0.006214954145102887
32	0.007964795603432828
33	0.009352600897970364
34	0.013214319978422645
35	0.017860450747091795
36	0.01562789440370532
37	0.010318030668083435
38	0.006456311587631156
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1657293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.3632495336019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.14771361441113	37.58898588561431
2	13.969098236963129	13.791201640612186
3	4.5433001383527145	6.728161753066594
4	1.9535329776385637	3.8573094338917104
5	1.0000481577608036	2.4682813378582718
6	0.5613883548441454	1.6627172067257876
7	0.3519378399091423	1.2160956788226283
8	0.23226318641343863	0.9172212502716853
9	0.17893324328853705	0.7949453704477878
>10	0.8646405506948643	7.7271108104468995
>50	0.08203852030240871	2.8560911080436973
>100	0.09402116778012751	10.14072562686668
>500	0.015200101415618984	5.279090809517116
>1k	0.005761328762371712	4.618561537521782
>5k	1.2258146302918536E-4	0.35350055029284855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5842	0.3525025448125346	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4179	0.25215818808140744	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2464	0.14867618459741277	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2442	0.1473487186635073	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2424	0.1462626101721301	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2215	0.13365168380002812	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2194	0.1323845572267547	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2094	0.12635062116354803	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2067	0.12472145842648222	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2031	0.12254924144372782	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2017	0.12170449039487886	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2010	0.12128211487045441	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1940	0.11705835962620972	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1875	0.11313630118512537	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1825	0.11011933315352204	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1802	0.10873152785898449	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1729	0.10432675453284361	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1696	0.10233555563198542	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1677	0.10118910777997614	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1660	0.10016333864923099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	6.033936063206687E-5
3	0.0	0.0	0.0	0.0	6.033936063206687E-5
4	0.0	0.0	0.0	0.0	6.033936063206687E-5
5	0.0	0.0	0.0	0.0	6.033936063206687E-5
6	0.0	0.0	0.0	0.0	6.033936063206687E-5
7	0.0	0.0	0.0	0.0	6.033936063206687E-5
8	0.0	0.0	0.0	0.0	6.033936063206687E-5
9	0.0	0.0	0.0	0.0	6.033936063206687E-5
10	0.0	0.0	0.0	0.0	6.033936063206687E-5
11	0.0	0.0	0.0	0.0	6.033936063206687E-5
12	0.0	0.0	0.0	0.0	3.0169680316033436E-4
13	0.0	0.0	0.0	0.0	3.0169680316033436E-4
14	0.0	0.0	0.0	6.033936063206687E-5	3.0169680316033436E-4
15	0.0	0.0	0.0	6.033936063206687E-5	4.223755244244681E-4
16	0.0	0.0	0.0	1.2067872126413374E-4	4.223755244244681E-4
17	0.0	0.0	0.0	1.2067872126413374E-4	4.223755244244681E-4
18	0.0	0.0	0.0	1.8101808189620062E-4	4.223755244244681E-4
19	0.0	0.0	0.0	1.8101808189620062E-4	4.223755244244681E-4
20	0.0	0.0	0.0	4.223755244244681E-4	4.82714885056535E-4
21	0.0	0.0	0.0	7.844116882168693E-4	6.033936063206687E-4
22	0.0	0.0	0.0	0.0012067872126413374	6.033936063206687E-4
23	0.0	0.0	0.0	0.0019911989008582066	7.240723275848025E-4
24	0.0	0.0	0.0	0.0035600222772919454	7.240723275848025E-4
25	0.0	0.0	0.0	0.003982397801716413	7.240723275848025E-4
26	0.0	0.0	0.0	0.0044047733261408815	7.240723275848025E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	55	5.83651E-5	20.363081	32
CTAGCGG	410	0.0	16.78003	29
ACCGTCG	440	0.0	16.724796	8
TAGGACG	750	0.0	16.637035	4
TCTAGCG	420	0.0	16.379515	28
ATACCGT	450	0.0	16.352148	6
GTCCTAC	905	0.0	16.281946	1
ATCGTTT	455	0.0	16.175383	29
TAGCGGC	440	0.0	15.999563	30
TACCGTC	460	0.0	15.997632	7
TTAGGAC	530	0.0	15.997149	3
CCGTCGT	465	0.0	15.828481	9
CATCGTT	465	0.0	15.826569	28
GACGTGA	465	0.0	15.825615	7
AGGACGT	800	0.0	15.596748	5
TGTAGGA	880	0.0	15.458321	2
TCCTACA	1060	0.0	15.399988	2
CCTACAG	1065	0.0	15.321213	3
CTAGACA	210	0.0	15.235379	4
GGACGTG	820	0.0	15.216341	6
>>END_MODULE