FastQCFastQC Report
Thu 2 Feb 2017
SRR4062863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657293
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT61190.3692165477076172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT46780.28226752903680885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT28310.17082072994938133No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27120.16364034603416536No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24840.1498829718100541No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22800.13757374224111246No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22730.13715136671668798No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22290.13449643484887705No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21800.13153980617790578No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21390.12906589239199104No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21060.12707469349113285No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20540.12393704673826535No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20390.12303195632878436No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20030.12085973934602993No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18800.11343799798828572No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18400.11102442356300304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.10981763635036171No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT18090.10915390338340897No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17880.10788677681013556No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17090.10311996732020227No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT16980.10245623435324955No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT16920.10209419818945716No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT16650.10046503545239134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG356.427259E-527.4317535
TATGCGA450.00885365417.77715912
TAGGCCG652.0992478E-417.2327675
CTAGCGG4500.017.0660729
GTACCGT957.688104E-716.8440596
GTAGGAC8900.016.7195263
TGTAGGA9250.016.6048262
TAGCGGC4550.016.52689630
TCTAGCG4750.016.50468828
CGGTCCT4200.016.38037912
AACCGCG4350.016.1857857
AGAACCG4950.016.163495
TAACCCC805.6220382E-516.0004084
ACCGTCG4650.015.8297928
CGCGGTC4350.015.81697210
TCGTTTA4800.015.66612130
GTCCTAA3700.015.5679641
GAACCGC4750.015.4965346
TACCGTC4700.015.3209267
CGTCGTA4900.015.02124410
Produced by FastQC (version 0.11.5)