##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657293
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181959979315668	32.0	32.0	32.0	32.0	32.0
2	31.296567354113	32.0	32.0	32.0	32.0	32.0
3	31.378716980039137	32.0	32.0	32.0	32.0	32.0
4	31.484237850518888	32.0	32.0	32.0	32.0	32.0
5	31.397785424786083	32.0	32.0	32.0	32.0	32.0
6	34.92732244690589	36.0	36.0	36.0	36.0	36.0
7	34.95527827608033	36.0	36.0	36.0	36.0	36.0
8	34.8932524303186	36.0	36.0	36.0	36.0	36.0
9	35.005442006935404	36.0	36.0	36.0	36.0	36.0
10	34.845554769132555	36.0	36.0	36.0	32.0	36.0
11	35.02948965572171	36.0	36.0	36.0	36.0	36.0
12	34.918539449572286	36.0	36.0	36.0	32.0	36.0
13	34.968377347879944	36.0	36.0	36.0	36.0	36.0
14	34.921678303112365	36.0	36.0	36.0	32.0	36.0
15	34.88860086900747	36.0	36.0	36.0	32.0	36.0
16	34.89883563135788	36.0	36.0	36.0	32.0	36.0
17	34.87120925509249	36.0	36.0	36.0	32.0	36.0
18	34.85715078745883	36.0	36.0	36.0	32.0	36.0
19	34.85757436977046	36.0	36.0	36.0	32.0	36.0
20	34.83710906882489	36.0	36.0	36.0	32.0	36.0
21	34.82019473925251	36.0	36.0	36.0	32.0	36.0
22	34.80998049228471	36.0	36.0	36.0	32.0	36.0
23	34.75425588595378	36.0	36.0	36.0	32.0	36.0
24	34.73168172435411	36.0	36.0	36.0	32.0	36.0
25	34.71105652410286	36.0	36.0	36.0	32.0	36.0
26	34.64361160036276	36.0	36.0	36.0	32.0	36.0
27	34.63069294325143	36.0	36.0	36.0	32.0	36.0
28	34.59287464558168	36.0	36.0	36.0	32.0	36.0
29	34.561038995518594	36.0	36.0	36.0	32.0	36.0
30	34.53358217285658	36.0	36.0	36.0	32.0	36.0
31	34.527691241078074	36.0	36.0	36.0	32.0	36.0
32	34.49725365400083	36.0	36.0	36.0	32.0	36.0
33	34.45980523661175	36.0	36.0	36.0	32.0	36.0
34	34.449174648055596	36.0	36.0	36.0	32.0	36.0
35	34.40680253883894	36.0	36.0	36.0	32.0	36.0
36	34.37814677308116	36.0	36.0	36.0	32.0	36.0
37	34.36195168868752	36.0	36.0	36.0	32.0	36.0
38	33.928333131196474	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	31.0
22	121.0
23	361.0
24	1015.0
25	2652.0
26	5534.0
27	10788.0
28	19454.0
29	30685.0
30	46479.0
31	67234.0
32	96504.0
33	154563.0
34	377008.0
35	844852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30651095457834	18.973171586828315	12.520388785398643	26.1999286731947
2	15.169134244819716	21.083417355893015	37.897643928985396	25.849804470301873
3	17.38690744485133	25.496155477637327	29.397939893549303	27.718997183962042
4	11.694641067430002	16.541653266267982	36.83238107535861	34.931324590943404
5	13.802585062007818	37.53269643050013	33.27472363030994	15.389994877182101
6	33.14714573016535	36.78851425430326	16.837920242033828	13.226419773497561
7	29.177022446926813	31.213181543088194	21.349711908679666	18.260084101305328
8	27.587305514238906	33.583787648853075	19.633429979091932	19.19547685781609
9	27.755531821171473	13.703469041714186	18.781701996705348	39.759297140409
10	15.99154995356819	26.786659256885876	31.45856224909776	25.763228540448168
11	37.28285924620751	20.97108410470547	22.845246859197935	18.90080978988909
12	24.808466859954684	24.140265554808014	28.97160114283301	22.079666442404296
13	29.67209078928949	19.5814487282838	25.420204575185483	25.32625590724123
14	23.52764417637678	20.452026286239068	25.177442974778753	30.842886562605404
15	24.73509512198507	28.09207545075011	22.496625521256654	24.67620390600817
16	25.055029530100914	25.745251892846888	24.683097486743435	24.516621090308767
17	23.40334509347472	26.322382342772222	25.654666978017765	24.619605585735293
18	24.650620017100174	24.44534551222988	27.396060925859217	23.507973544810724
19	25.64096994315429	24.62889784727263	25.912617744719853	23.81751446485323
20	25.869113065704134	24.033469036555395	25.502913486028117	24.594504411712354
21	27.167572159884923	24.043198181129217	24.369091264544814	24.42013839444105
22	25.825367029245882	24.69340062378831	25.206828243406566	24.274404103559238
23	23.810227769155947	24.291012084774437	26.22132973549622	25.677430410573393
24	24.634268050368885	25.2258954813663	25.85825198079036	24.281584487474454
25	24.574470716935913	24.538508371201402	26.02545482411336	24.86156608774933
26	24.053381025563976	25.661968040654248	26.50828791287962	23.77636302090216
27	24.949707051873844	24.979273392103977	25.345292616259073	24.725726939763106
28	23.83766781130434	25.03504208368707	26.24852696535857	24.87876313965002
29	24.01434145923503	24.868927823866994	26.05429456348395	25.06243615341403
30	23.74583130442233	25.206345528521513	26.563981142743014	24.483842024313144
31	24.9797712293481	24.736784624082766	25.140937661596354	25.14250648497278
32	24.284782473587953	25.296914908830242	25.12494773102885	25.293354886552947
33	23.787887838782883	24.704020351259555	26.2551642950281	25.252927514929468
34	24.72163944456412	24.8224665161803	26.288230264654473	24.16766377460111
35	25.425196389534015	24.764902766137308	26.012117350402132	23.79778349392654
36	23.881051811598795	25.55619314146623	25.74197803285237	24.820777014082605
37	25.091157689074894	25.364615671459422	25.18800236288936	24.35622427657632
38	24.15334672869564	25.282660246547074	25.766522455333707	24.797470569423577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	410.0
1	309.0
2	208.0
3	208.0
4	703.5
5	1199.0
6	1199.0
7	1461.0
8	1723.0
9	1765.5
10	1808.0
11	1808.0
12	2328.5
13	2849.0
14	3577.0
15	4305.0
16	4305.0
17	6897.5
18	9490.0
19	9490.0
20	10942.5
21	12395.0
22	13251.0
23	14107.0
24	14107.0
25	16463.5
26	18820.0
27	18820.0
28	24726.0
29	30632.0
30	38360.5
31	46089.0
32	46089.0
33	58289.5
34	70490.0
35	70490.0
36	76425.0
37	82360.0
38	90735.0
39	99110.0
40	99110.0
41	102425.0
42	105740.0
43	117903.0
44	130066.0
45	130066.0
46	130711.0
47	131356.0
48	131356.0
49	139205.5
50	147055.0
51	146141.0
52	145227.0
53	145227.0
54	137722.0
55	130217.0
56	130217.0
57	126976.0
58	123735.0
59	110374.5
60	97014.0
61	97014.0
62	94020.5
63	91027.0
64	74866.0
65	58705.0
66	58705.0
67	49364.5
68	40024.0
69	40024.0
70	32303.5
71	24583.0
72	19241.0
73	13899.0
74	13899.0
75	10380.0
76	6861.0
77	6861.0
78	6836.5
79	6812.0
80	5280.5
81	3749.0
82	3749.0
83	3630.0
84	3511.0
85	3511.0
86	2182.5
87	854.0
88	752.0
89	650.0
90	650.0
91	388.5
92	127.0
93	86.5
94	46.0
95	46.0
96	31.0
97	16.0
98	16.0
99	20.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007964795603432828
2	0.0
3	0.0
4	2.4135744252826746E-4
5	1.2067872126413373E-4
6	2.4135744252826746E-4
7	3.6203616379240123E-4
8	0.002292895704018541
9	0.0038013797198202127
10	0.001448144655169605
11	0.0044047733261408815
12	4.827148850565349E-4
13	3.0169680316033436E-4
14	0.0
15	0.0
16	6.0339360632066865E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.0339360632066865E-5
22	0.0
23	6.0339360632066865E-5
24	0.0
25	2.4135744252826746E-4
26	0.0
27	1.8101808189620062E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.8101808189620062E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1657293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.83549539321149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77643896847003	34.553560674353264
2	15.209950672828779	14.247311493364922
3	5.134094046051278	7.213735141264474
4	2.1357734545913165	4.0012003117382
5	1.1080765847150806	2.594865788937434
6	0.6215140283382504	1.7465350446631462
7	0.3965240864450767	1.2999981416797046
8	0.260304158573688	0.9753179375769406
9	0.20121057848789967	0.8481417409651899
>10	0.9414488223918657	7.97561769646144
>50	0.09013966658818076	2.9788984759776045
>100	0.10060285963970428	10.33469387024189
>500	0.01577563724987899	5.072272429116653
>1k	0.008017127126987683	5.78727067751553
>5k	1.2930850204818844E-4	0.37058057614371787
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6119	0.3692165477076172	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4678	0.28226752903680885	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2831	0.17082072994938133	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2712	0.16364034603416536	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2484	0.1498829718100541	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2280	0.13757374224111246	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2273	0.13715136671668798	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2229	0.13449643484887705	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2180	0.13153980617790578	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2139	0.12906589239199104	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2106	0.12707469349113285	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2054	0.12393704673826535	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2039	0.12303195632878436	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2003	0.12085973934602993	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1880	0.11343799798828572	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1840	0.11102442356300304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1820	0.10981763635036171	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1809	0.10915390338340897	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1788	0.10788677681013556	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1709	0.10311996732020227	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1698	0.10245623435324955	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1692	0.10209419818945716	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1665	0.10046503545239134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.033936063206687E-5
2	0.0	0.0	0.0	0.0	6.033936063206687E-5
3	0.0	0.0	0.0	0.0	6.033936063206687E-5
4	0.0	0.0	0.0	0.0	6.033936063206687E-5
5	0.0	0.0	0.0	0.0	6.033936063206687E-5
6	0.0	0.0	0.0	0.0	6.033936063206687E-5
7	0.0	0.0	0.0	0.0	6.033936063206687E-5
8	0.0	0.0	0.0	0.0	6.033936063206687E-5
9	0.0	0.0	0.0	0.0	1.2067872126413374E-4
10	0.0	0.0	0.0	0.0	1.2067872126413374E-4
11	0.0	0.0	0.0	0.0	1.2067872126413374E-4
12	0.0	0.0	0.0	0.0	2.413574425282675E-4
13	0.0	0.0	0.0	0.0	2.413574425282675E-4
14	0.0	0.0	0.0	6.033936063206687E-5	3.0169680316033436E-4
15	0.0	0.0	0.0	6.033936063206687E-5	3.0169680316033436E-4
16	0.0	0.0	0.0	1.2067872126413374E-4	3.0169680316033436E-4
17	0.0	0.0	0.0	1.8101808189620062E-4	3.0169680316033436E-4
18	0.0	0.0	0.0	2.413574425282675E-4	3.0169680316033436E-4
19	0.0	0.0	0.0	2.413574425282675E-4	3.0169680316033436E-4
20	0.0	0.0	0.0	6.033936063206687E-4	3.0169680316033436E-4
21	0.0	0.0	0.0	9.05090409481003E-4	3.6203616379240123E-4
22	0.0	0.0	0.0	0.0011464478520092705	3.6203616379240123E-4
23	0.0	0.0	0.0	0.00193085954022614	3.6203616379240123E-4
24	0.0	0.0	0.0	0.0031979861134995443	3.6203616379240123E-4
25	0.0	0.0	0.0	0.0034996829166598787	3.6203616379240123E-4
26	0.0	0.0	0.0	0.003982397801716413	4.223755244244681E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	35	6.427259E-5	27.431753	5
TATGCGA	45	0.008853654	17.777159	12
TAGGCCG	65	2.0992478E-4	17.232767	5
CTAGCGG	450	0.0	17.06607	29
GTACCGT	95	7.688104E-7	16.844059	6
GTAGGAC	890	0.0	16.719526	3
TGTAGGA	925	0.0	16.604826	2
TAGCGGC	455	0.0	16.526896	30
TCTAGCG	475	0.0	16.504688	28
CGGTCCT	420	0.0	16.380379	12
AACCGCG	435	0.0	16.185785	7
AGAACCG	495	0.0	16.16349	5
TAACCCC	80	5.6220382E-5	16.000408	4
ACCGTCG	465	0.0	15.829792	8
CGCGGTC	435	0.0	15.816972	10
TCGTTTA	480	0.0	15.666121	30
GTCCTAA	370	0.0	15.567964	1
GAACCGC	475	0.0	15.496534	6
TACCGTC	470	0.0	15.320926	7
CGTCGTA	490	0.0	15.021244	10
>>END_MODULE