FastQCFastQC Report
Thu 2 Feb 2017
SRR4062862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459936
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23600.5131148681555695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17590.3824445140193418No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13180.2865616085716274No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11560.2513393167745077No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11380.24742572879705005No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11230.24416440548250193No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11070.24068566061365057No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10560.229597161344187No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10460.22742294580115494No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10340.22481388714951644No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10280.2235093578236972No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10140.2204654560634523No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10110.21981319140054267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10030.21807381896611702No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9850.21416023098865927No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9360.2035065748278021No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9000.19567939887288666No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8760.19046128156960968No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8680.18872190913518402No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8220.17872051763723648No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8090.17589403743129478No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8000.17393724344256592No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7990.1737198218882627No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7890.17154560634523064No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7770.16893654769359215No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT7710.16763201836777292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.16393585194461838No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT7470.16241390106449594No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7050.1532821957837612No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7000.15219508801224518No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG6950.15110798024072913No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG6940.15089055868642592No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG6940.15089055868642592No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT6690.14545501982884573No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC6600.1434982258401169No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG6430.13980205941696236No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT6210.1350187852222918No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT6110.13284456967925973No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA6060.13175746190774368No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT5870.12762645237598275No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC5830.1267567661587699No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC5790.1258870799415571No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC5630.12240833507270577No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG5570.12110380574688652No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT5550.1206689626382801No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC5300.11523342378069992No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC5260.1143637375634871No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG5180.11262436512906145No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCG5150.1119721004661518No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC5080.11045014958602935No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA5050.10979788492311975No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC4920.10697140471717805No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA4910.10675398316287485No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC4890.10631914005426842No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG4840.10523203228275238No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG4650.10110102275099145No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA4630.10066617964238503No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC4620.10044875808808182No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT4610.10023133653377862No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA4600.1000139149794754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA350.002052215222.8809341
GACAGTC350.00206654522.853577
TAGAACA350.00206654522.853574
TAATGTG350.00206654522.853575
GCCCTAG502.7705368E-522.4233151
GGTCCTA1300.020.92208313
GTATTAC400.004452567520.0208171
ACCATAC2150.018.6240161
AGGCCCG2250.018.4880110
TCTAGCG2350.018.38210528
AAGACGG2450.018.2828565
CAAGACG2450.018.2828564
TCGTTTA2200.018.1829330
CGCGGTC1500.018.13247110
AGAACCG1500.018.13055
CCCTAGA450.00881165817.7885362
CTAGGAC450.00884485317.7769323
AGAGCCG450.00885039717.7755
TAGCGGC2450.017.6337330
GTATTAA550.001353487717.4727131
Produced by FastQC (version 0.11.5)