##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062862_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459936
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.096787379113614	32.0	32.0	32.0	32.0	32.0
2	30.71455376400195	32.0	32.0	32.0	32.0	32.0
3	30.774255548598067	32.0	32.0	32.0	32.0	32.0
4	30.818424737354764	32.0	32.0	32.0	32.0	32.0
5	30.766976274959994	32.0	32.0	32.0	32.0	32.0
6	34.39224587768733	36.0	36.0	36.0	32.0	36.0
7	34.26319748834621	36.0	36.0	36.0	32.0	36.0
8	34.23278890976136	36.0	36.0	36.0	32.0	36.0
9	34.35095535030961	36.0	36.0	36.0	32.0	36.0
10	34.083168092952064	36.0	36.0	36.0	32.0	36.0
11	34.39725092186739	36.0	36.0	36.0	32.0	36.0
12	34.16306399151186	36.0	36.0	36.0	32.0	36.0
13	34.28274803450915	36.0	36.0	36.0	32.0	36.0
14	34.164005426841996	36.0	36.0	36.0	32.0	36.0
15	34.115107319279204	36.0	36.0	36.0	32.0	36.0
16	34.116329228414386	36.0	36.0	36.0	32.0	36.0
17	33.98982467125861	36.0	36.0	36.0	32.0	36.0
18	34.04122747512697	36.0	36.0	36.0	32.0	36.0
19	34.03958376817644	36.0	36.0	36.0	32.0	36.0
20	34.01726544562722	36.0	36.0	36.0	32.0	36.0
21	33.96855649481667	36.0	36.0	36.0	32.0	36.0
22	33.95003652682112	36.0	36.0	36.0	32.0	36.0
23	33.89974474709525	36.0	36.0	36.0	32.0	36.0
24	33.862928755305084	36.0	36.0	36.0	32.0	36.0
25	33.84298467612885	36.0	36.0	36.0	32.0	36.0
26	33.78187399986085	36.0	36.0	36.0	32.0	36.0
27	33.795056268698254	36.0	36.0	36.0	32.0	36.0
28	33.7645411535518	36.0	36.0	36.0	32.0	36.0
29	33.73731997495304	36.0	36.0	36.0	27.0	36.0
30	33.69435747582272	36.0	36.0	36.0	27.0	36.0
31	33.71524516454463	36.0	36.0	36.0	27.0	36.0
32	33.64931642663327	36.0	36.0	36.0	27.0	36.0
33	33.58199619077437	36.0	36.0	36.0	27.0	36.0
34	33.607158387253875	36.0	36.0	36.0	27.0	36.0
35	33.509388262714815	36.0	36.0	36.0	21.0	36.0
36	33.466438808877754	36.0	36.0	36.0	21.0	36.0
37	33.47452471648229	36.0	36.0	36.0	21.0	36.0
38	32.80423145828985	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	4.0
11	0.0
12	6.0
13	3.0
14	91.0
15	175.0
16	242.0
17	291.0
18	357.0
19	462.0
20	670.0
21	839.0
22	1227.0
23	1819.0
24	2619.0
25	3801.0
26	5621.0
27	7960.0
28	10556.0
29	14084.0
30	18390.0
31	24286.0
32	32176.0
33	46718.0
34	99028.0
35	188508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.9909654947208	16.670730379884617	11.17927506258844	25.159029062806137
2	17.470082643908956	20.169116543357053	35.38373183258898	26.977068980145013
3	18.694876924415066	24.6568670087849	27.985561450813258	28.662694615986776
4	12.528727884692739	14.783690171530203	34.49455230160093	38.19302964217612
5	15.297629659907727	36.238895145825275	31.676936256071524	16.78653893819548
6	34.98857442279636	34.46632444290799	16.478052238043368	14.06704889625228
7	30.772542266750158	30.11245042788562	20.852466430112017	18.26254087525221
8	30.210355811147227	29.997717068716234	19.409264352571558	20.382662767564984
9	28.626001500211984	13.16707795666779	18.19017904703926	40.01674149608097
10	17.65557666969609	24.855522748916147	28.786086699396	28.702813881991762
11	39.324982769079575	20.436632610685564	20.669059789663187	19.569324830571674
12	26.019081413450916	23.602160356928536	26.79203520581661	23.58672302380393
13	30.390630436295318	17.682529051562174	25.045012525224408	26.8818279869181
14	25.403735216669965	19.39625086704951	23.483505945948295	31.71650797033223
15	26.296812580055196	25.793375507514586	22.173847803252876	25.735964109177345
16	27.23333007164835	24.376094023505875	22.78569642410603	25.60487948073975
17	24.853320459458168	24.863976200728068	24.36054679175202	25.922156548061743
18	26.934124210988646	21.922719830575915	26.10244155934518	25.04071439909026
19	26.5114600252662	24.057464291383184	24.79914242785979	24.631933255490832
20	26.894551337106527	21.59287340376725	24.712172572519467	26.800402686606756
21	29.50881694244526	22.57637363832054	22.868604727011807	25.04620469222239
22	27.44778280779116	22.524167982152875	24.10862219670976	25.91942701334621
23	24.42774483518921	22.111326379511667	25.246634528616564	28.21429425668256
24	25.26752300121557	24.375091058936604	24.46337745478584	25.89400848506199
25	25.790892880441874	22.75866568085939	24.399164963249685	27.05127647544905
26	25.396210987787484	24.47809749138861	25.04088236317456	25.084809157649353
27	26.94237877799522	23.140247879973906	23.864535768645357	26.052837573385517
28	25.15785765848822	22.990685093540446	25.679918592414136	26.171538655557196
29	24.54119662877371	23.878649769945987	25.828020491767635	25.75213310951267
30	24.689460238082294	24.071315975430778	26.198836766864165	25.040387019622763
31	25.961005329315146	24.10389881476553	23.24851220567547	26.68658365024385
32	25.184387910415307	23.89845618612742	23.423352902804957	27.493803000652317
33	24.56327272569135	23.1468716976373	24.81941647930628	27.47043909736507
34	26.149798319145003	23.77137762701545	24.701610185154983	25.37721386868456
35	27.628288486545966	22.844244577721142	25.010111729170927	24.51735520656197
36	25.174615324397436	24.895404768324823	23.814224951507825	26.115754955769916
37	26.762180391781676	24.434277961030574	24.27272074955588	24.530820897631873
38	25.162151643042428	23.856893428917463	24.648789216997276	26.332165711042837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	391.0
1	293.0
2	195.0
3	195.0
4	473.0
5	751.0
6	751.0
7	857.5
8	964.0
9	878.0
10	792.0
11	792.0
12	1033.0
13	1274.0
14	1444.0
15	1614.0
16	1614.0
17	2370.5
18	3127.0
19	3127.0
20	3356.0
21	3585.0
22	3027.5
23	2470.0
24	2470.0
25	2337.0
26	2204.0
27	2204.0
28	2859.5
29	3515.0
30	4698.5
31	5882.0
32	5882.0
33	8724.5
34	11567.0
35	11567.0
36	12754.5
37	13942.0
38	17078.5
39	20215.0
40	20215.0
41	21381.5
42	22548.0
43	27821.5
44	33095.0
45	33095.0
46	34995.0
47	36895.0
48	36895.0
49	39926.0
50	42957.0
51	43385.0
52	43813.0
53	43813.0
54	41097.0
55	38381.0
56	38381.0
57	39320.0
58	40259.0
59	36316.5
60	32374.0
61	32374.0
62	32944.0
63	33514.0
64	27596.5
65	21679.0
66	21679.0
67	18646.0
68	15613.0
69	15613.0
70	12549.0
71	9485.0
72	7485.5
73	5486.0
74	5486.0
75	4123.0
76	2760.0
77	2760.0
78	2910.5
79	3061.0
80	2365.0
81	1669.0
82	1669.0
83	1814.5
84	1960.0
85	1960.0
86	1318.0
87	676.0
88	589.0
89	502.0
90	502.0
91	359.5
92	217.0
93	184.0
94	151.0
95	151.0
96	134.0
97	117.0
98	117.0
99	176.5
100	236.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12740903082167954
2	0.05500765323871147
3	0.012175607040979615
4	0.0036961664231545256
5	4.3484310860641485E-4
6	6.522646629096222E-4
7	0.0
8	2.1742155430320742E-4
9	2.1742155430320742E-4
10	4.3484310860641485E-4
11	0.001521950880122452
12	0.002609058651638489
13	0.013914979475405275
14	0.009349126835037918
15	0.02108989076741112
16	0.011088499269463578
17	0.019567939887288666
18	0.005435538857580185
19	0.007174911292005844
20	0.005435538857580185
21	0.005652960411883392
22	0.006522646629096222
23	0.009783969943644333
24	0.015002087246921312
25	0.016524038127043762
26	0.01739372434425659
27	0.007827175954915467
28	0.006087803520489808
29	0.009566548389341126
30	0.0023916370973352816
31	0.0058703819661865995
32	0.007827175954915467
33	0.008262019063521881
34	0.011088499269463578
35	0.01608919501843735
36	0.014784665692618102
37	0.008044597509218675
38	0.0058703819661865995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	459936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.91872748860887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84639354136951	36.31517119921605
2	11.848895590507455	10.644746241419673
3	3.228887519698858	4.351125557661696
4	1.2722432503527665	2.2859019144727166
5	0.673150627088114	1.5118534788478581
6	0.3860559611846902	1.0404685509484837
7	0.24250378631295866	0.762507304464334
8	0.17247193702342387	0.619777595087015
9	0.12757320308513106	0.5157383349806962
>10	0.8019421595568791	7.5592201663712375
>50	0.16985297128285537	5.418309252316518
>100	0.2052794481504121	19.686712539666033
>500	0.017955885535615244	5.549320028814631
>1k	0.006794118851313875	3.7391478357330943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2360	0.5131148681555695	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1759	0.3824445140193418	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1318	0.2865616085716274	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1156	0.2513393167745077	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1138	0.24742572879705005	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1123	0.24416440548250193	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1107	0.24068566061365057	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1056	0.229597161344187	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1046	0.22742294580115494	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1034	0.22481388714951644	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1028	0.2235093578236972	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1014	0.2204654560634523	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1011	0.21981319140054267	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1003	0.21807381896611702	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	985	0.21416023098865927	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	936	0.2035065748278021	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	900	0.19567939887288666	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	876	0.19046128156960968	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	868	0.18872190913518402	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	822	0.17872051763723648	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	809	0.17589403743129478	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	800	0.17393724344256592	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	799	0.1737198218882627	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	789	0.17154560634523064	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	777	0.16893654769359215	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	771	0.16763201836777292	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	754	0.16393585194461838	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	747	0.16241390106449594	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	705	0.1532821957837612	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	700	0.15219508801224518	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	695	0.15110798024072913	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	694	0.15089055868642592	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	694	0.15089055868642592	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	669	0.14545501982884573	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	660	0.1434982258401169	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	643	0.13980205941696236	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	621	0.1350187852222918	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	611	0.13284456967925973	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	606	0.13175746190774368	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	587	0.12762645237598275	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	583	0.1267567661587699	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	579	0.1258870799415571	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	563	0.12240833507270577	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	557	0.12110380574688652	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	555	0.1206689626382801	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	530	0.11523342378069992	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	526	0.1143637375634871	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	518	0.11262436512906145	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCG	515	0.1119721004661518	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC	508	0.11045014958602935	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA	505	0.10979788492311975	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	492	0.10697140471717805	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA	491	0.10675398316287485	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC	489	0.10631914005426842	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	484	0.10523203228275238	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	465	0.10110102275099145	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA	463	0.10066617964238503	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC	462	0.10044875808808182	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	461	0.10023133653377862	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA	460	0.1000139149794754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.174215543032074E-4
2	0.0	0.0	0.0	0.0	2.174215543032074E-4
3	0.0	0.0	0.0	0.0	2.174215543032074E-4
4	0.0	0.0	0.0	0.0	2.174215543032074E-4
5	0.0	0.0	0.0	0.0	2.174215543032074E-4
6	0.0	0.0	0.0	0.0	2.174215543032074E-4
7	0.0	0.0	0.0	0.0	2.174215543032074E-4
8	0.0	0.0	0.0	0.0	4.348431086064148E-4
9	0.0	0.0	0.0	0.0	4.348431086064148E-4
10	0.0	0.0	0.0	0.0	6.522646629096222E-4
11	0.0	0.0	0.0	0.0	8.696862172128296E-4
12	0.0	0.0	0.0	0.0	0.001087107771516037
13	0.0	0.0	0.0	0.0	0.0013045293258192444
14	0.0	0.0	0.0	0.0	0.0013045293258192444
15	0.0	0.0	0.0	0.0	0.0013045293258192444
16	0.0	0.0	0.0	2.174215543032074E-4	0.001521950880122452
17	0.0	0.0	0.0	4.348431086064148E-4	0.001521950880122452
18	0.0	0.0	0.0	8.696862172128296E-4	0.001521950880122452
19	0.0	0.0	0.0	8.696862172128296E-4	0.001521950880122452
20	0.0	0.0	0.0	0.0013045293258192444	0.001521950880122452
21	0.0	0.0	0.0	0.0013045293258192444	0.001521950880122452
22	0.0	0.0	0.0	0.0017393724344256592	0.001521950880122452
23	0.0	0.0	0.0	0.0034787448688513183	0.001521950880122452
24	0.0	0.0	0.0	0.005435538857580185	0.001521950880122452
25	0.0	0.0	0.0	0.006957489737702637	0.001521950880122452
26	0.0	0.0	0.0	0.008696862172128296	0.001521950880122452
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAA	35	0.0020522152	22.880934	1
GACAGTC	35	0.002066545	22.85357	7
TAGAACA	35	0.002066545	22.85357	4
TAATGTG	35	0.002066545	22.85357	5
GCCCTAG	50	2.7705368E-5	22.423315	1
GGTCCTA	130	0.0	20.922083	13
GTATTAC	40	0.0044525675	20.020817	1
ACCATAC	215	0.0	18.624016	1
AGGCCCG	225	0.0	18.48801	10
TCTAGCG	235	0.0	18.382105	28
AAGACGG	245	0.0	18.282856	5
CAAGACG	245	0.0	18.282856	4
TCGTTTA	220	0.0	18.18293	30
CGCGGTC	150	0.0	18.132471	10
AGAACCG	150	0.0	18.1305	5
CCCTAGA	45	0.008811658	17.788536	2
CTAGGAC	45	0.008844853	17.776932	3
AGAGCCG	45	0.008850397	17.775	5
TAGCGGC	245	0.0	17.63373	30
GTATTAA	55	0.0013534877	17.472713	1
>>END_MODULE