FastQCFastQC Report
Thu 2 Feb 2017
SRR4062862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459936
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26320.5722535309260418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21430.46593439087177346No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13200.2869964516802338No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12880.28003896194253114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12730.276777638627983No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12480.27134209977040286No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11970.26025360050093926No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11940.25960133583802963No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11560.2513393167745077No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10930.23764175885340572No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10830.23546754331037362No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10480.22785778890976136No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10370.22546615181242607No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10350.22503130870381965No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10130.22024803450914912No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9910.21546476031447853No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9560.2078550059138663No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9530.20720274125095667No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9200.2000278299589508No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8650.1880696444722744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.1854605858206359No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT8280.18002504696305574No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG8110.1763288805399012No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8020.17437208655117234No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA7940.1726327141167467No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT7860.170893341682321No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT7860.170893341682321No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT7520.16350100883601196No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7510.16328358728170875No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7470.16241390106449594No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG7340.15958742085855424No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7300.1587177346413414No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG6790.14762923537187783No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG6620.14393306894872332No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6500.1413240102970848No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC6410.13936721630835597No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT6340.1378452654282335No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC6240.13567104988520143No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6180.13436652055938217No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC6140.13349683434216933No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC6110.13284456967925973No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC6010.13067035413622763No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC5910.12849613859319556No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA5780.12566965838725386No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT5710.12414770750713143No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC5480.11914701175815767No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT5480.11914701175815767No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC5420.1178424824323384No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT5410.1176250608780352No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCG5410.1176250608780352No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG5330.11588568844360955No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC5330.11588568844360955No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC5320.11566826688930634No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA5320.11566826688930634No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT5320.11566826688930634No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG5290.11501600222639673No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG5260.1143637375634871No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCG5240.11392889445488068No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5130.1115372573575454No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC5050.10979788492311975No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG5050.10979788492311975No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG4870.105884296945662No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA4860.1056668753913588No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG4850.10544945383705558No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA4770.10371008140262993No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC4720.1026229736311139No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAG4720.1026229736311139No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT4710.10240555207681068No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAA4690.10197070896820427No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA4660.10131844430529464No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT4640.10088360119668822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTTT200.003751693331.9991315
GAGTTGC400.004477347720.001636
ATATAAA507.183016E-419.2015671
TCGTTTA2250.018.48838630
TGTACCC450.0088382817.7792265
ATCGTTT2250.017.77729429
GGCATCG2350.017.70164726
TATTAGC1550.017.547912
GTATGGA550.001362214317.4559691
ATGGGCG753.2246233E-517.0680585
TTGGATC2100.016.7614517
ATTAGCT1551.0913936E-1116.5174753
AGCTCTA1551.0913936E-1116.5174756
GGCCGTT1950.016.4115947
TTAATAT2150.016.3734283
GCATCGT2550.016.31328227
GCGATGC2650.016.30144332
TACGCTA2200.016.0013059
ACGATCA1203.0926458E-816.0013059
CGATCAG1304.776666E-915.99956610
Produced by FastQC (version 0.11.5)