FastQCFastQC Report
Thu 2 Feb 2017
SRR4062861_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062861_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362598
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30540.22413066803268464No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT30490.2237637219488066No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28390.20835198642593045No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25190.18486743705773823No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23610.17327194080719333No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23460.1721711025555593No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23190.1701895937026181No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22910.1681346956329013No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21740.15954815727015598No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21700.15925460040305356No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21580.15837392980174636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20350.14934705613834748No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20080.14736554728540627No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19320.1417879668104606No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18790.13789833832135376No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18390.13496276965032974No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18360.13474260200000293No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18160.13327481766449092No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18120.1329812607973885No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17460.12813757249019886No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16320.11977120177778039No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15780.11580818407189795No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15650.11485412425381514No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC15240.11184516636601552No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC14850.1089829869117671No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC14710.10795553787690867No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT14680.10773537022658188No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG14600.10714825649237707No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT14280.10479980155555783No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14130.10369896330392384No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14030.10296507113616782No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC13940.10230456818518742No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG13870.1017908436677582No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG13740.1008367838496754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5300.019.62277828
CTAGCGG5400.019.260129
TCGTTTA4200.018.6674830
ATCGTTT4300.018.60546329
TAGCGGC5600.018.57223930
AAGACGG7400.018.156295
CATCGTT4450.017.97765428
TCGAACT550.001364758717.45466419
CGTTTAT4500.017.42298131
CAAGACG7550.017.3718644
CGCAAGA7000.016.9144022
GCGCAAG6950.016.8188361
GCATCGT4700.016.68035327
CGGCATC4950.016.48496225
GTATTAG4000.016.0124551
AGCGGCG6800.016.00069831
CGCTTCG4000.016.00069832
CTCGAAC703.697865E-415.99952218
CTAAGGT908.609528E-615.9948254
GGCATCG5150.015.84418726
Produced by FastQC (version 0.11.5)