##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062861_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1362598
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250555923317076	32.0	32.0	32.0	32.0	32.0
2	30.853145241663352	32.0	32.0	32.0	32.0	32.0
3	30.89692778060734	32.0	32.0	32.0	32.0	32.0
4	30.966704046241077	32.0	32.0	32.0	32.0	32.0
5	30.876563740736447	32.0	32.0	32.0	32.0	32.0
6	34.54457587637733	36.0	36.0	36.0	32.0	36.0
7	34.46253260315955	36.0	36.0	36.0	32.0	36.0
8	34.45500066784187	36.0	36.0	36.0	32.0	36.0
9	34.59522911379585	36.0	36.0	36.0	32.0	36.0
10	34.29649023409692	36.0	36.0	36.0	32.0	36.0
11	34.57454729861632	36.0	36.0	36.0	32.0	36.0
12	34.37535942368916	36.0	36.0	36.0	32.0	36.0
13	34.47930791033012	36.0	36.0	36.0	32.0	36.0
14	34.36853862988203	36.0	36.0	36.0	32.0	36.0
15	34.304456633577914	36.0	36.0	36.0	32.0	36.0
16	34.328683147927705	36.0	36.0	36.0	32.0	36.0
17	34.23843055692141	36.0	36.0	36.0	32.0	36.0
18	34.26264899845736	36.0	36.0	36.0	32.0	36.0
19	34.25830509071641	36.0	36.0	36.0	32.0	36.0
20	34.241412360799	36.0	36.0	36.0	32.0	36.0
21	34.23114814494077	36.0	36.0	36.0	32.0	36.0
22	34.20526156650751	36.0	36.0	36.0	32.0	36.0
23	34.1437012236918	36.0	36.0	36.0	32.0	36.0
24	34.1187488899881	36.0	36.0	36.0	32.0	36.0
25	34.092744888808	36.0	36.0	36.0	32.0	36.0
26	34.04018646732198	36.0	36.0	36.0	32.0	36.0
27	34.061374668097265	36.0	36.0	36.0	32.0	36.0
28	34.043407520046266	36.0	36.0	36.0	32.0	36.0
29	33.99631219185703	36.0	36.0	36.0	32.0	36.0
30	33.983564484903106	36.0	36.0	36.0	32.0	36.0
31	33.99570599692646	36.0	36.0	36.0	32.0	36.0
32	33.95174732386221	36.0	36.0	36.0	32.0	36.0
33	33.90389975620102	36.0	36.0	36.0	32.0	36.0
34	33.92059139966447	36.0	36.0	36.0	32.0	36.0
35	33.88236515832256	36.0	36.0	36.0	32.0	36.0
36	33.84000857186052	36.0	36.0	36.0	32.0	36.0
37	33.84407727003856	36.0	36.0	36.0	32.0	36.0
38	33.23717853688322	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	6.0
10	11.0
11	10.0
12	7.0
13	8.0
14	126.0
15	271.0
16	424.0
17	512.0
18	659.0
19	845.0
20	1175.0
21	1741.0
22	2679.0
23	4240.0
24	6190.0
25	9435.0
26	13828.0
27	19444.0
28	26925.0
29	36452.0
30	48193.0
31	64666.0
32	88632.0
33	132232.0
34	293526.0
35	610354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15189054945701	16.811488780143723	11.118826139987362	25.917794530411907
2	17.513481284804623	19.07954251744637	36.3355870673653	27.071389130383704
3	19.132610557730178	22.980579595473685	28.005634038517858	29.881175808278275
4	12.602958693225478	14.496818858018099	35.25845815343313	37.64176429532329
5	15.253931291295347	35.90279709348279	32.933385097384175	15.909886517837682
6	35.71168563536074	34.81472131137916	16.086206036125162	13.38738701713494
7	31.41579541434818	29.782151448923305	20.188125918282577	18.613927218445937
8	29.203774280029737	31.89665864518407	18.908461893554264	19.991105181231934
9	27.655506816782417	13.523040262205956	18.28594451901632	40.535508401995315
10	16.584555701764366	25.64115172095433	30.20519056764816	27.569102009633145
11	39.142493126998964	20.54245701897433	21.110938236196386	19.204111617830318
12	25.164984044692474	23.34208649039012	27.663303379824388	23.829626085093018
13	29.788197609809735	18.241312035654676	25.11343935196807	26.857051002567523
14	24.16009159834712	19.385747943073977	23.890801265348962	32.56335919322994
15	25.776842741968043	26.51934958896025	21.64530414886253	26.058503520209182
16	26.908292872687507	25.000018349499243	22.845199954493243	25.246488823320014
17	24.81296501829243	25.481240714315078	24.211414125398587	25.494380141993904
18	26.259651234477293	23.595763731908523	25.518524498722954	24.62606053489123
19	26.22333097735101	24.28327861729526	24.561299210489036	24.932091194864693
20	26.41464705290885	23.227521498877444	24.110375460637805	26.247455987575897
21	28.191501463834946	23.366313961587267	23.541504436274924	24.90068013830286
22	26.94119123582295	23.23609303772988	24.32343168922445	25.499284037222726
23	24.781866436496124	23.12435961798736	25.25659318237199	26.83718076314453
24	25.472034644744568	24.375733998825602	24.501394597768645	25.65083675866119
25	25.521683951534353	23.70778211744706	24.462497990629544	26.308035940389047
26	25.179670233279527	25.19383685773993	25.085862222603915	24.540630686376623
27	26.279195905692355	23.701097339624816	24.197766274219674	25.821940480463155
28	25.165099705690235	23.75505500877058	25.308438103206583	25.771407182332606
29	24.77663777557427	24.293472237614985	25.255619744391268	25.67427024241948
30	24.67060190332763	24.589651399093327	25.565167357644906	25.174579339934137
31	25.6497895807615	24.46786024111241	23.75359444792656	26.128755730199526
32	25.521944187971833	23.980074891633187	23.774056845421143	26.723924074973837
33	24.850473662153046	23.60743620588799	24.797628423105404	26.744461708853564
34	26.266562343110976	23.893813104252114	24.683359414222256	25.156265138414653
35	26.820021521256276	23.44972819504776	25.07894308024082	24.651307203455143
36	24.86435661878044	24.808792744547105	24.26746702133594	26.05938361533651
37	26.406290941358577	24.446610450014568	24.27552373745566	24.87157487117119
38	25.075761078658676	23.904023979326343	24.824092646863466	26.196122295151515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	86.5
2	133.0
3	133.0
4	318.5
5	504.0
6	504.0
7	638.0
8	772.0
9	834.0
10	896.0
11	896.0
12	1134.0
13	1372.0
14	1861.5
15	2351.0
16	2351.0
17	3504.0
18	4657.0
19	4657.0
20	5569.5
21	6482.0
22	6946.0
23	7410.0
24	7410.0
25	8670.5
26	9931.0
27	9931.0
28	12828.0
29	15725.0
30	19642.0
31	23559.0
32	23559.0
33	31896.0
34	40233.0
35	40233.0
36	45322.5
37	50412.0
38	59222.5
39	68033.0
40	68033.0
41	72618.5
42	77204.0
43	89953.5
44	102703.0
45	102703.0
46	104326.5
47	105950.0
48	105950.0
49	115003.0
50	124056.0
51	128379.5
52	132703.0
53	132703.0
54	126937.0
55	121171.0
56	121171.0
57	119652.5
58	118134.0
59	106134.0
60	94134.0
61	94134.0
62	91927.5
63	89721.0
64	74551.0
65	59381.0
66	59381.0
67	50170.5
68	40960.0
69	40960.0
70	32610.5
71	24261.0
72	19000.0
73	13739.0
74	13739.0
75	10298.0
76	6857.0
77	6857.0
78	6842.5
79	6828.0
80	5416.0
81	4004.0
82	4004.0
83	4034.5
84	4065.0
85	4065.0
86	2738.0
87	1411.0
88	1195.0
89	979.0
90	979.0
91	742.0
92	505.0
93	414.0
94	323.0
95	323.0
96	305.5
97	288.0
98	288.0
99	499.5
100	711.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11874375274292198
2	0.05210634391067651
3	0.01210922076797412
4	0.002715401020697227
5	3.669460838780036E-4
6	1.4677843355120145E-4
7	0.0
8	5.137245174292052E-4
9	3.669460838780036E-4
10	8.07281384531608E-4
11	0.0014677843355120144
12	0.0030823471045752305
13	0.014384286488017742
14	0.009393819747276893
15	0.02355793858496783
16	0.011962442334422918
17	0.020989315997821806
18	0.005724358908496857
19	0.00799942462854048
20	0.006238083425926061
21	0.005944526558823659
22	0.007118754027233271
23	0.009540598180828095
24	0.014531064921568942
25	0.016952909075163766
26	0.017686801242919777
27	0.008880095229847689
28	0.006458251076252864
29	0.009173652096950091
30	0.002715401020697227
31	0.005871137342048058
32	0.007045364810457671
33	0.009467208964052494
34	0.012476166851852122
35	0.016439184557734566
36	0.014824621788671347
37	0.009613987397603696
38	0.006678418726579666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1362598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.41847659687733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.50636430013259	37.52740085975018
2	13.442141171019314	13.016959954018398
3	4.162169642472515	6.045777402788663
4	1.763089409980588	3.4146441334138946
5	0.887412151826698	2.1483572252502765
6	0.5157209296906311	1.4982253058847386
7	0.33225230925640575	1.1261005461992812
8	0.23107890725344366	0.895079093030628
9	0.16314819610076778	0.7109448403255001
>10	0.7789831714601606	6.9576756531284145
>50	0.09001800159064217	3.0958047913354374
>100	0.10109112105931926	10.8546656765488
>500	0.01799207587656337	6.030122249210549
>1k	0.008538612280402955	6.678242269115292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3054	0.22413066803268464	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3049	0.2237637219488066	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2839	0.20835198642593045	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2519	0.18486743705773823	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2361	0.17327194080719333	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2346	0.1721711025555593	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2319	0.1701895937026181	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2291	0.1681346956329013	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2174	0.15954815727015598	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2170	0.15925460040305356	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2158	0.15837392980174636	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2035	0.14934705613834748	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2008	0.14736554728540627	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1932	0.1417879668104606	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1879	0.13789833832135376	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1839	0.13496276965032974	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1836	0.13474260200000293	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1816	0.13327481766449092	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1812	0.1329812607973885	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1746	0.12813757249019886	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1632	0.11977120177778039	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1578	0.11580818407189795	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1565	0.11485412425381514	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1524	0.11184516636601552	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1485	0.1089829869117671	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1471	0.10795553787690867	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1468	0.10773537022658188	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1460	0.10714825649237707	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1428	0.10479980155555783	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1413	0.10369896330392384	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1403	0.10296507113616782	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1394	0.10230456818518742	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1387	0.1017908436677582	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1374	0.1008367838496754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.338921677560072E-5
2	0.0	0.0	0.0	0.0	7.338921677560072E-5
3	0.0	0.0	0.0	0.0	7.338921677560072E-5
4	0.0	0.0	0.0	0.0	7.338921677560072E-5
5	0.0	0.0	0.0	0.0	7.338921677560072E-5
6	0.0	0.0	0.0	0.0	7.338921677560072E-5
7	0.0	0.0	0.0	0.0	7.338921677560072E-5
8	0.0	0.0	0.0	0.0	7.338921677560072E-5
9	0.0	0.0	0.0	0.0	7.338921677560072E-5
10	0.0	0.0	0.0	0.0	7.338921677560072E-5
11	0.0	0.0	0.0	0.0	7.338921677560072E-5
12	0.0	0.0	0.0	0.0	2.2016765032680218E-4
13	0.0	0.0	0.0	0.0	2.935568671024029E-4
14	0.0	0.0	0.0	0.0	2.935568671024029E-4
15	0.0	0.0	0.0	0.0	3.6694608387800366E-4
16	0.0	0.0	0.0	1.4677843355120145E-4	3.6694608387800366E-4
17	0.0	0.0	0.0	1.4677843355120145E-4	3.6694608387800366E-4
18	0.0	0.0	0.0	1.4677843355120145E-4	3.6694608387800366E-4
19	0.0	0.0	0.0	1.4677843355120145E-4	3.6694608387800366E-4
20	0.0	0.0	0.0	3.6694608387800366E-4	3.6694608387800366E-4
21	0.0	0.0	0.0	3.6694608387800366E-4	3.6694608387800366E-4
22	0.0	0.0	0.0	5.137245174292051E-4	3.6694608387800366E-4
23	0.0	0.0	0.0	8.07281384531608E-4	3.6694608387800366E-4
24	0.0	0.0	0.0	0.0012476166851852124	3.6694608387800366E-4
25	0.0	0.0	0.0	0.0015411735522876152	3.6694608387800366E-4
26	0.0	0.0	0.0	0.002128287286492421	4.4033530065360437E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	530	0.0	19.622778	28
CTAGCGG	540	0.0	19.2601	29
TCGTTTA	420	0.0	18.66748	30
ATCGTTT	430	0.0	18.605463	29
TAGCGGC	560	0.0	18.572239	30
AAGACGG	740	0.0	18.15629	5
CATCGTT	445	0.0	17.977654	28
TCGAACT	55	0.0013647587	17.454664	19
CGTTTAT	450	0.0	17.422981	31
CAAGACG	755	0.0	17.371864	4
CGCAAGA	700	0.0	16.914402	2
GCGCAAG	695	0.0	16.818836	1
GCATCGT	470	0.0	16.680353	27
CGGCATC	495	0.0	16.484962	25
GTATTAG	400	0.0	16.012455	1
AGCGGCG	680	0.0	16.000698	31
CGCTTCG	400	0.0	16.000698	32
CTCGAAC	70	3.697865E-4	15.999522	18
CTAAGGT	90	8.609528E-6	15.994825	4
GGCATCG	515	0.0	15.844187	26
>>END_MODULE