FastQCFastQC Report
Thu 2 Feb 2017
SRR4062861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362598
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32450.23814800843682438No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC30070.2206813748442314No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT29690.2178925846067586No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25060.18391337723965542No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24630.1807576409183046No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24210.17767529381372937No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23820.17481311435948094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23260.1707033182200473No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23170.1700428152690669No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22700.16659352208061365No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21970.1612361092559948No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21620.15866748666884875No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20700.1519156787254935No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20110.14758571493573305No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18830.13819189518845615No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18530.13599021868518815No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18330.13452243434967615No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17600.1291650215250573No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16910.12410116556754083No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16880.12388099791721403No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC16530.121312375330068No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16460.12079865081263881No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16410.1204317047287608No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16370.1201381478616584No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15830.11617513015577596No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG15810.11602835172222475No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC15590.11441378895316154No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15430.11323956148475192No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT15210.11162499871568869No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14880.10920315456209388No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG14550.10678131040849906No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG14240.10450624468845543No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC14030.10296507113616782No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC14030.10296507113616782No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG14010.10281829270261662No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC13720.10069000541612419No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG13680.10039644854902179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAG350.002065427422.8596325
CCCTTAA604.71567E-621.3364411
ATCGTTT4550.020.39483329
TCTAGCG5950.019.36061128
CATCGTT4950.019.06998628
TCGTTTA5000.018.87928630
TAGCGGC6250.018.43130130
CGGCATC5150.018.32940325
GCATCGT5150.018.32940327
TATACAC1750.018.2863643
CTAGCGG6300.018.2850229
CGTTTAT5300.017.81064631
GGCATCG5350.017.64419226
CAAGACG5450.017.6153074
AAGACGG5700.017.4054035
GTTCTAA854.9783885E-616.9436441
GGTTATA1354.4201443E-1016.5950091
AGCGGCG7200.016.44382331
GCGCAAG5850.016.4126471
ACCGTCG4200.016.3827388
Produced by FastQC (version 0.11.5)