##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1362598
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226123185268143	32.0	32.0	32.0	32.0	32.0
2	31.317509639673624	32.0	32.0	32.0	32.0	32.0
3	31.397721118040685	32.0	32.0	32.0	32.0	32.0
4	31.494644054959718	32.0	32.0	32.0	32.0	32.0
5	31.414279927021763	32.0	32.0	32.0	32.0	32.0
6	34.963948281151154	36.0	36.0	36.0	36.0	36.0
7	34.97999776896781	36.0	36.0	36.0	36.0	36.0
8	34.92299342872953	36.0	36.0	36.0	36.0	36.0
9	35.02493618807601	36.0	36.0	36.0	36.0	36.0
10	34.87364431769311	36.0	36.0	36.0	32.0	36.0
11	35.05676876085243	36.0	36.0	36.0	36.0	36.0
12	34.93480395538523	36.0	36.0	36.0	32.0	36.0
13	34.996025240019435	36.0	36.0	36.0	36.0	36.0
14	34.92702249673051	36.0	36.0	36.0	32.0	36.0
15	34.909034065806644	36.0	36.0	36.0	32.0	36.0
16	34.91871263571501	36.0	36.0	36.0	32.0	36.0
17	34.87700554382143	36.0	36.0	36.0	32.0	36.0
18	34.86844762725323	36.0	36.0	36.0	32.0	36.0
19	34.87375880487128	36.0	36.0	36.0	32.0	36.0
20	34.86349752458172	36.0	36.0	36.0	32.0	36.0
21	34.85328835063606	36.0	36.0	36.0	32.0	36.0
22	34.84004453257674	36.0	36.0	36.0	32.0	36.0
23	34.77625976260056	36.0	36.0	36.0	32.0	36.0
24	34.74710296066778	36.0	36.0	36.0	32.0	36.0
25	34.717083101545725	36.0	36.0	36.0	32.0	36.0
26	34.65320439337207	36.0	36.0	36.0	32.0	36.0
27	34.64473380997183	36.0	36.0	36.0	32.0	36.0
28	34.61722019260266	36.0	36.0	36.0	32.0	36.0
29	34.573574157601875	36.0	36.0	36.0	32.0	36.0
30	34.56035309019975	36.0	36.0	36.0	32.0	36.0
31	34.557350003449294	36.0	36.0	36.0	32.0	36.0
32	34.52999490678835	36.0	36.0	36.0	32.0	36.0
33	34.49195140459622	36.0	36.0	36.0	32.0	36.0
34	34.47717301801412	36.0	36.0	36.0	32.0	36.0
35	34.457793127540185	36.0	36.0	36.0	32.0	36.0
36	34.42328845338097	36.0	36.0	36.0	32.0	36.0
37	34.42419040685514	36.0	36.0	36.0	32.0	36.0
38	33.981958728840056	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	31.0
22	99.0
23	361.0
24	908.0
25	2131.0
26	4441.0
27	9011.0
28	15455.0
29	24680.0
30	36967.0
31	53360.0
32	77046.0
33	124364.0
34	307272.0
35	706468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41285370590281	18.06257899070974	11.808643216670507	26.715924086716935
2	16.445349251943714	19.967811489522223	35.710825936923435	27.87601332161063
3	18.139319153558127	23.683287367220558	28.266810900940705	29.91058257828061
4	12.09230335668585	15.224490934203438	35.43792208097203	37.24528362813868
5	14.622434496454566	36.46526708537661	32.69056610974037	16.221732308428457
6	35.66982754255294	35.228537858222204	15.75777724448241	13.343857354742452
7	31.103395435470876	29.962263153954883	20.211156074369914	18.723185336204327
8	29.092055328165124	32.039299524938706	18.758866575514784	20.109778571381394
9	27.501664167662966	13.353927178811972	18.11847405916731	41.02593459435776
10	16.230019521789547	25.85873856947849	30.343245901158095	27.567996007573868
11	39.198964284246436	20.46676197913303	21.121643758403447	19.212629978217088
12	24.97093782749986	23.404149138849668	27.665720433642843	23.959192600007633
13	29.754799107880025	18.32031040725908	25.061995586369264	26.86289489849163
14	24.011281398682076	19.371391541299445	23.923434441731487	32.69389261828699
15	25.740313724223874	26.581721094556134	21.571732822152974	26.106232359067015
16	26.838842914554277	25.0686924077276	22.767496748852924	25.324967928865195
17	24.835131124513612	25.516109666974412	24.13286970918789	25.515889499324086
18	26.186079826918874	23.659289093334937	25.39465051321079	24.759980566535397
19	26.171768929647627	24.315462080525585	24.55691260371731	24.955856386109478
20	26.44382275623478	23.124501870691137	24.12369605709094	26.307979315983143
21	28.174214386205165	23.44273471906954	23.496896000798476	24.88615489392682
22	26.811928709557865	23.276322017914346	24.36351226886933	25.548237003658457
23	24.672023108830494	22.999920739529543	25.297740489477437	27.03031566216252
24	25.43237257063345	24.27069465829247	24.530198928810993	25.76673384226309
25	25.356341342053927	23.69745148605825	24.665602033761974	26.280605138125846
26	25.2514866831499	25.062582700534346	25.045996725370745	24.639933890945013
27	26.411678279287067	23.575258440126877	24.176829850036473	25.836233430549584
28	25.08736986257135	23.689452061429712	25.423786032270705	25.799392043728233
29	24.90176853334586	24.08362554473146	25.29425406466177	25.72035185726091
30	24.69070114589923	24.415711750641055	25.64857720325437	25.245009900205346
31	25.705527235472236	24.28030864569007	23.87160409746675	26.14256002137094
32	25.586930261162866	23.950277337850196	23.852669679538646	26.610122721448292
33	24.701489360765244	23.5688735782674	24.9067590000866	26.822878060880758
34	26.148651326363314	23.74383347106043	24.898832964674835	25.20868223790142
35	26.798072505610605	23.37101624984038	25.07474691728595	24.75616432726307
36	24.947196458529955	24.697159396975486	24.222331164437346	26.133312980057216
37	26.496516213879662	24.27311650244606	24.234000049904665	24.996367233769607
38	25.11980789638617	23.69994671942862	24.89523689305283	26.285008491132384
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	28.5
2	45.0
3	45.0
4	213.0
5	381.0
6	381.0
7	551.5
8	722.0
9	814.5
10	907.0
11	907.0
12	1125.0
13	1343.0
14	1862.5
15	2382.0
16	2382.0
17	3629.5
18	4877.0
19	4877.0
20	5699.0
21	6521.0
22	6897.0
23	7273.0
24	7273.0
25	8515.0
26	9757.0
27	9757.0
28	12701.0
29	15645.0
30	19613.0
31	23581.0
32	23581.0
33	32236.0
34	40891.0
35	40891.0
36	45794.5
37	50698.0
38	59508.0
39	68318.0
40	68318.0
41	72489.5
42	76661.0
43	90171.0
44	103681.0
45	103681.0
46	104518.0
47	105355.0
48	105355.0
49	115390.5
50	125426.0
51	128922.5
52	132419.0
53	132419.0
54	127096.0
55	121773.0
56	121773.0
57	120350.5
58	118928.0
59	106721.5
60	94515.0
61	94515.0
62	91610.5
63	88706.0
64	74044.0
65	59382.0
66	59382.0
67	50262.5
68	41143.0
69	41143.0
70	33006.0
71	24869.0
72	19354.0
73	13839.0
74	13839.0
75	10263.5
76	6688.0
77	6688.0
78	6729.5
79	6771.0
80	5341.0
81	3911.0
82	3911.0
83	3638.5
84	3366.0
85	3366.0
86	2181.5
87	997.0
88	804.5
89	612.0
90	612.0
91	360.0
92	108.0
93	88.0
94	68.0
95	68.0
96	41.0
97	14.0
98	14.0
99	13.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007045364810457671
2	0.0
3	0.0
4	2.935568671024029E-4
5	0.0
6	7.338921677560072E-5
7	2.935568671024029E-4
8	0.002715401020697227
9	0.0040364069226580404
10	0.001321005901960813
11	0.004550131440087245
12	1.4677843355120145E-4
13	7.338921677560072E-5
14	7.338921677560072E-5
15	0.0
16	1.4677843355120145E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	7.338921677560072E-5
22	2.201676503268022E-4
23	1.4677843355120145E-4
24	0.0
25	0.0
26	7.338921677560072E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1362598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.14129045826981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64473085947131	34.90345498224511
2	14.456213296786022	13.34056673307412
3	4.6018158700977825	6.37001168092972
4	1.9287802640744165	3.5598564157934396
5	0.9420796890352249	2.1734386283305405
6	0.5421327252422076	1.5008822125400447
7	0.35974295137307044	1.161930280673405
8	0.243080417687262	0.8972835325780399
9	0.18328575063188723	0.7611336951091127
>10	0.8637944880944419	7.27489527590731
>50	0.09306169862521083	3.0267343712266204
>100	0.11177474989331274	11.36551088812308
>500	0.0200456786601887	6.527810538984344
>1k	0.009461560327609066	7.136490764485195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3245	0.23814800843682438	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3007	0.2206813748442314	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2969	0.2178925846067586	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2506	0.18391337723965542	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2463	0.1807576409183046	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2421	0.17767529381372937	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2382	0.17481311435948094	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2326	0.1707033182200473	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2317	0.1700428152690669	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2270	0.16659352208061365	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2197	0.1612361092559948	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2162	0.15866748666884875	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2070	0.1519156787254935	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2011	0.14758571493573305	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1883	0.13819189518845615	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1853	0.13599021868518815	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1833	0.13452243434967615	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1760	0.1291650215250573	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1691	0.12410116556754083	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1688	0.12388099791721403	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1653	0.121312375330068	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1646	0.12079865081263881	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1641	0.1204317047287608	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1637	0.1201381478616584	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1583	0.11617513015577596	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1581	0.11602835172222475	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1559	0.11441378895316154	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1543	0.11323956148475192	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1521	0.11162499871568869	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1488	0.10920315456209388	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1455	0.10678131040849906	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1424	0.10450624468845543	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1403	0.10296507113616782	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1403	0.10296507113616782	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1401	0.10281829270261662	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1372	0.10069000541612419	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1368	0.10039644854902179	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	7.338921677560072E-5
13	0.0	0.0	0.0	0.0	7.338921677560072E-5
14	0.0	0.0	0.0	0.0	7.338921677560072E-5
15	0.0	0.0	0.0	0.0	2.2016765032680218E-4
16	0.0	0.0	0.0	7.338921677560072E-5	2.2016765032680218E-4
17	0.0	0.0	0.0	7.338921677560072E-5	2.2016765032680218E-4
18	0.0	0.0	0.0	7.338921677560072E-5	2.2016765032680218E-4
19	0.0	0.0	0.0	7.338921677560072E-5	2.2016765032680218E-4
20	0.0	0.0	0.0	2.935568671024029E-4	2.2016765032680218E-4
21	0.0	0.0	0.0	2.935568671024029E-4	2.2016765032680218E-4
22	0.0	0.0	0.0	4.4033530065360437E-4	2.2016765032680218E-4
23	0.0	0.0	0.0	8.806706013072087E-4	2.2016765032680218E-4
24	0.0	0.0	0.0	0.0011742274684096116	2.935568671024029E-4
25	0.0	0.0	0.0	0.0015411735522876152	2.935568671024029E-4
26	0.0	0.0	0.0	0.002128287286492421	2.935568671024029E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACAG	35	0.0020654274	22.859632	5
CCCTTAA	60	4.71567E-6	21.336441	1
ATCGTTT	455	0.0	20.394833	29
TCTAGCG	595	0.0	19.360611	28
CATCGTT	495	0.0	19.069986	28
TCGTTTA	500	0.0	18.879286	30
TAGCGGC	625	0.0	18.431301	30
CGGCATC	515	0.0	18.329403	25
GCATCGT	515	0.0	18.329403	27
TATACAC	175	0.0	18.286364	3
CTAGCGG	630	0.0	18.28502	29
CGTTTAT	530	0.0	17.810646	31
GGCATCG	535	0.0	17.644192	26
CAAGACG	545	0.0	17.615307	4
AAGACGG	570	0.0	17.405403	5
GTTCTAA	85	4.9783885E-6	16.943644	1
GGTTATA	135	4.4201443E-10	16.595009	1
AGCGGCG	720	0.0	16.443823	31
GCGCAAG	585	0.0	16.412647	1
ACCGTCG	420	0.0	16.382738	8
>>END_MODULE