FastQCFastQC Report
Thu 2 Feb 2017
SRR4062860_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062860_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762014
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40900.23212074364902888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27840.15800101474789643No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25060.1422236145683292No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24360.13825088790440937No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22240.12621920143653798No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21910.12434634458069005No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21090.11969257906009828No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20980.1190682934414823No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20590.11685491715729841No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20480.11623063153868243No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20260.1149820603014505No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19840.11259842430309862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC5000.020.15892830
TCTAGCG5200.019.69126128
CTAGCGG5200.019.69126129
ACGATTA450.008854805517.77683332
CGGTCCA6350.017.3858910
ACTATAC1201.6643753E-917.3309353
AGCGGCG6000.016.53245431
ATCGTTT4700.015.99914929
GTATCAA31350.015.9904241
CGTTTAT4700.015.65874231
GCGGCGC6450.015.62707532
CCGGTCC7150.015.2164079
TCGTTTA5050.015.20711130
CGCTTCG4000.015.1991932
CATCGTT4950.015.19111228
GTCCTAT5350.014.9681941
TCTAGAT3700.014.7069282
CGCCGGT6650.014.6750777
CTTATAC1205.429447E-714.6646383
GCCGGTC7000.014.62737758
Produced by FastQC (version 0.11.5)