##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062860_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762014
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225499343365037	32.0	32.0	32.0	32.0	32.0
2	30.786299654826806	32.0	32.0	32.0	32.0	32.0
3	30.832998489228803	32.0	32.0	32.0	32.0	32.0
4	30.914173780685058	32.0	32.0	32.0	32.0	32.0
5	30.79474453664954	32.0	32.0	32.0	32.0	32.0
6	34.45799636098238	36.0	36.0	36.0	32.0	36.0
7	34.39109394136482	36.0	36.0	36.0	32.0	36.0
8	34.373043006468734	36.0	36.0	36.0	32.0	36.0
9	34.53630731651394	36.0	36.0	36.0	32.0	36.0
10	34.206556815099084	36.0	36.0	36.0	32.0	36.0
11	34.52045727218967	36.0	36.0	36.0	32.0	36.0
12	34.308553167000944	36.0	36.0	36.0	32.0	36.0
13	34.417037549077364	36.0	36.0	36.0	32.0	36.0
14	34.30315139380277	36.0	36.0	36.0	32.0	36.0
15	34.23777790641845	36.0	36.0	36.0	32.0	36.0
16	34.25922836027409	36.0	36.0	36.0	32.0	36.0
17	34.17870402845834	36.0	36.0	36.0	32.0	36.0
18	34.20719528902722	36.0	36.0	36.0	32.0	36.0
19	34.19063355909771	36.0	36.0	36.0	32.0	36.0
20	34.17796737142838	36.0	36.0	36.0	32.0	36.0
21	34.17020352846232	36.0	36.0	36.0	32.0	36.0
22	34.140260520063975	36.0	36.0	36.0	32.0	36.0
23	34.083446556043256	36.0	36.0	36.0	32.0	36.0
24	34.07179454873798	36.0	36.0	36.0	32.0	36.0
25	34.04639236691649	36.0	36.0	36.0	32.0	36.0
26	33.99181504800757	36.0	36.0	36.0	32.0	36.0
27	34.00865089607687	36.0	36.0	36.0	32.0	36.0
28	33.99481672676835	36.0	36.0	36.0	32.0	36.0
29	33.95133750356126	36.0	36.0	36.0	32.0	36.0
30	33.93470937234324	36.0	36.0	36.0	32.0	36.0
31	33.95082729195114	36.0	36.0	36.0	32.0	36.0
32	33.89642363795066	36.0	36.0	36.0	32.0	36.0
33	33.86182799909649	36.0	36.0	36.0	32.0	36.0
34	33.874970346433116	36.0	36.0	36.0	32.0	36.0
35	33.842720886440176	36.0	36.0	36.0	32.0	36.0
36	33.797869369936905	36.0	36.0	36.0	32.0	36.0
37	33.791986896812396	36.0	36.0	36.0	32.0	36.0
38	33.195167007753625	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	3.0
8	3.0
9	7.0
10	12.0
11	20.0
12	5.0
13	7.0
14	183.0
15	329.0
16	531.0
17	629.0
18	755.0
19	1006.0
20	1427.0
21	2211.0
22	3429.0
23	5373.0
24	8246.0
25	12556.0
26	18174.0
27	26044.0
28	36717.0
29	48681.0
30	65457.0
31	87872.0
32	119736.0
33	179094.0
34	389636.0
35	753869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.855306034436225	16.92941102270577	11.27123570057996	25.944047242278046
2	17.117549390595684	19.201800729558947	37.01103423839224	26.66961564145312
3	18.999914293611923	23.238010045242756	28.28384593510494	29.478229726040382
4	12.653550242797243	14.816591031371809	35.655552553854676	36.87430617197627
5	14.978445095720119	36.160934204761375	33.33847896609103	15.52214173342747
6	35.06816926795576	35.16272032353941	16.43690079119824	13.332209617306589
7	30.96537880254005	30.15011807517879	20.423004824595505	18.461498297685658
8	28.806419940862995	32.324334141122925	19.05443215418755	19.81481376382654
9	27.6665442685026	13.860542142520272	18.372210357608843	40.100703231368286
10	16.126751131098455	26.147954261170803	31.10946399667651	26.615830611054232
11	38.07690648723258	20.877317841705715	21.80581662489557	19.239959046166142
12	25.04937671825304	23.4902075184283	28.263630811980267	23.196784951338394
13	29.671658260309847	18.936780336958115	25.08982484580778	26.30173655692426
14	23.8315892474937	19.44361041377318	24.73410187761904	31.990698461114086
15	25.474870102924243	26.87491449471414	21.97013261337646	25.68008278898516
16	26.281949042727753	25.49327999482356	23.293804336289163	24.93096662615952
17	24.443746867288848	25.622015232284678	24.775480755858275	25.1587571445682
18	25.393504710478265	24.37280176261959	25.786938475744968	24.446755051157172
19	25.904700714747474	24.6299835232913	25.17355031355693	24.29176544840429
20	26.19411938980411	23.867460948984238	24.567433592557236	25.370986068654418
21	27.341083735790093	23.799993983723134	24.18855465759155	24.670367622895224
22	26.218669493915947	23.801961631215374	24.824209759799217	25.155159115069463
23	24.64292930783569	23.69931140015302	25.40497526444005	26.252784027571234
24	25.10424175686951	24.659745596757805	24.998609353093766	25.23740329327892
25	25.22967415644237	24.195977261052313	24.97293860881461	25.601409973690707
26	25.029630739678627	25.173924494971857	25.308908975730038	24.48753578961948
27	25.731147831288993	24.381318986317247	24.618115132193207	25.269418050200553
28	24.794073260143946	24.29382154510467	25.511532067701413	25.400573127049974
29	24.628069058172265	24.67444158565664	25.426618738928347	25.270870617242746
30	24.602490845484493	24.87485612711212	25.621803297668748	24.900849729734638
31	25.468966828517996	24.725510172228354	24.235358887113666	25.570164112139988
32	25.055394870071783	24.551903177032017	24.309437106389932	26.083264846506264
33	24.61803389691981	24.20778819788736	25.019028067321614	26.155149837871217
34	25.655735239110435	24.320113338566614	25.075419982165975	24.948731440156976
35	26.189678816803426	24.142663827043325	25.142700154847265	24.524957201305988
36	24.795445915133683	25.023740477556807	24.671251856809018	25.50956175050049
37	25.935997484443796	24.771644595952974	24.559593197327562	24.73276472227567
38	24.88623038616398	24.444716878540934	25.138515710933802	25.530537024361283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	102.5
2	136.0
3	136.0
4	402.5
5	669.0
6	669.0
7	900.5
8	1132.0
9	1140.0
10	1148.0
11	1148.0
12	1595.0
13	2042.0
14	2711.0
15	3380.0
16	3380.0
17	5110.0
18	6840.0
19	6840.0
20	8366.5
21	9893.0
22	10470.5
23	11048.0
24	11048.0
25	13166.0
26	15284.0
27	15284.0
28	19462.0
29	23640.0
30	29608.5
31	35577.0
32	35577.0
33	45905.5
34	56234.0
35	56234.0
36	63721.0
37	71208.0
38	81865.0
39	92522.0
40	92522.0
41	99490.0
42	106458.0
43	121572.0
44	136686.0
45	136686.0
46	140123.5
47	143561.0
48	143561.0
49	153694.0
50	163827.0
51	166827.0
52	169827.0
53	169827.0
54	162392.5
55	154958.0
56	154958.0
57	149847.0
58	144736.0
59	130298.0
60	115860.0
61	115860.0
62	109698.0
63	103536.0
64	86409.0
65	69282.0
66	69282.0
67	57786.5
68	46291.0
69	46291.0
70	37583.0
71	28875.0
72	22703.5
73	16532.0
74	16532.0
75	12751.0
76	8970.0
77	8970.0
78	8436.0
79	7902.0
80	6275.0
81	4648.0
82	4648.0
83	4570.0
84	4492.0
85	4492.0
86	3064.0
87	1636.0
88	1375.0
89	1114.0
90	1114.0
91	805.5
92	497.0
93	414.0
94	331.0
95	331.0
96	314.5
97	298.0
98	298.0
99	586.5
100	875.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11719543658563439
2	0.05391557615319743
3	0.010499349040359497
4	0.002837661902799864
5	1.1350647611199456E-4
6	1.7025971416799186E-4
7	5.675323805599728E-5
8	2.2701295222398913E-4
9	2.8376619027998645E-4
10	5.675323805599729E-4
11	0.0017025971416799184
12	0.002837661902799864
13	0.014301815990111315
14	0.008796751898679579
15	0.024233632649910842
16	0.010499349040359497
17	0.02048791893821502
18	0.005164544663095753
19	0.007548180661447639
20	0.004994284948927761
21	0.007207661233111655
22	0.006413115900327693
23	0.010102076373967516
24	0.013847790085663338
25	0.01583415341762324
26	0.018728568558479104
27	0.009137271327015563
28	0.005732077043655726
29	0.01106688142091947
30	0.002383635998351886
31	0.006186102948103705
32	0.00669688209060768
33	0.008115713042007611
34	0.012088439705927422
35	0.015777400179567247
36	0.014472075704279308
37	0.01004532313591152
38	0.0068103885667196735
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1762014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.48366872775586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25270370627703	42.634943857840305
2	13.38139206530123	14.58134664804186
3	3.9620092131885913	6.475943924030515
4	1.6165027006328512	3.5229199055521185
5	0.8028706806814372	2.187167009873764
6	0.46112693426549906	1.507433227678026
7	0.2938876273493562	1.1208453292181941
8	0.2055895468984927	0.8961018213685523
9	0.1425853551071794	0.6991715927780094
>10	0.73731514953377	7.165129222064999
>50	0.059943544904006656	2.324459419691972
>100	0.0672174237740603	8.108366084856405
>500	0.011935341229008578	4.4703408432575475
>1k	0.004920710857573713	4.305831113747732
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4090	0.23212074364902888	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2784	0.15800101474789643	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2506	0.1422236145683292	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2436	0.13825088790440937	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2224	0.12621920143653798	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2191	0.12434634458069005	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2109	0.11969257906009828	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2098	0.1190682934414823	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2059	0.11685491715729841	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2048	0.11623063153868243	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2026	0.1149820603014505	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1984	0.11259842430309862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.675323805599728E-5
2	0.0	0.0	0.0	0.0	5.675323805599728E-5
3	0.0	0.0	0.0	0.0	5.675323805599728E-5
4	0.0	0.0	0.0	0.0	5.675323805599728E-5
5	0.0	0.0	0.0	0.0	5.675323805599728E-5
6	0.0	0.0	0.0	0.0	1.7025971416799186E-4
7	0.0	0.0	0.0	0.0	1.7025971416799186E-4
8	0.0	0.0	0.0	0.0	1.7025971416799186E-4
9	0.0	0.0	0.0	0.0	1.7025971416799186E-4
10	0.0	0.0	0.0	0.0	1.7025971416799186E-4
11	0.0	0.0	0.0	5.675323805599728E-5	1.7025971416799186E-4
12	0.0	0.0	0.0	5.675323805599728E-5	3.405194283359837E-4
13	0.0	0.0	0.0	5.675323805599728E-5	4.5402590444797825E-4
14	0.0	0.0	0.0	5.675323805599728E-5	4.5402590444797825E-4
15	0.0	0.0	0.0	5.675323805599728E-5	5.107791425039756E-4
16	0.0	0.0	0.0	5.675323805599728E-5	5.107791425039756E-4
17	0.0	0.0	0.0	5.675323805599728E-5	5.107791425039756E-4
18	0.0	0.0	0.0	5.675323805599728E-5	5.107791425039756E-4
19	0.0	0.0	0.0	1.1350647611199456E-4	5.107791425039756E-4
20	0.0	0.0	0.0	1.7025971416799186E-4	5.107791425039756E-4
21	0.0	0.0	0.0	1.7025971416799186E-4	5.107791425039756E-4
22	0.0	0.0	0.0	3.405194283359837E-4	5.675323805599729E-4
23	0.0	0.0	0.0	9.080518088959565E-4	6.242856186159701E-4
24	0.0	0.0	0.0	0.0015323374275119266	6.242856186159701E-4
25	0.0	0.0	0.0	0.001816103617791913	6.242856186159701E-4
26	0.0	0.0	0.0	0.0024971424744638804	6.242856186159701E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	500	0.0	20.158928	30
TCTAGCG	520	0.0	19.691261	28
CTAGCGG	520	0.0	19.691261	29
ACGATTA	45	0.0088548055	17.776833	32
CGGTCCA	635	0.0	17.38589	10
ACTATAC	120	1.6643753E-9	17.330935	3
AGCGGCG	600	0.0	16.532454	31
ATCGTTT	470	0.0	15.999149	29
GTATCAA	3135	0.0	15.990424	1
CGTTTAT	470	0.0	15.658742	31
GCGGCGC	645	0.0	15.627075	32
CCGGTCC	715	0.0	15.216407	9
TCGTTTA	505	0.0	15.207111	30
CGCTTCG	400	0.0	15.19919	32
CATCGTT	495	0.0	15.191112	28
GTCCTAT	535	0.0	14.968194	1
TCTAGAT	370	0.0	14.706928	2
CGCCGGT	665	0.0	14.675077	7
CTTATAC	120	5.429447E-7	14.664638	3
GCCGGTC	700	0.0	14.6273775	8
>>END_MODULE