FastQCFastQC Report
Thu 2 Feb 2017
SRR4062860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762014
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44560.2528924287775239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31280.1775241286391595No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25720.145969328280025No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23950.1359240051441135No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22850.1296811489579538No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22680.12871634391100184No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22160.12576517553209No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22130.12559491581792198No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21570.12241673448678614No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20370.11560634592006647No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19690.11174712573225865No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19090.10834193144889882No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18310.10391517888053102No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18070.1025531011671871No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17890.10153154288217914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA2500.018.56097210
TCGCGTA2550.018.1975489
CTAGCGG4750.018.18887729
TCTAGCG4800.017.99940928
GCGTAAC2700.017.77870811
CAAGACG5150.017.7091624
TAGCGGC4900.017.63207430
ATCGTTT4050.016.59204929
CGGTCCA5700.016.56227110
AAGACGG5800.016.553535
ACCGTCC1203.1088348E-816.0017458
CGCAAGA5400.015.7036342
GTATAGG1950.015.5901171
GTATCAA30250.015.4978921
AGCGGCG6200.015.48336231
CATCGTT4350.015.44776928
TAATACT2700.015.4077774
CGTTTAT4450.015.10062831
CGCCGGT6100.014.9524527
CCCCTAT756.245558E-414.9336911
Produced by FastQC (version 0.11.5)