##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062860_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762014
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201663550913896	32.0	32.0	32.0	32.0	32.0
2	31.266764622755552	32.0	32.0	32.0	32.0	32.0
3	31.358671383995812	32.0	32.0	32.0	32.0	32.0
4	31.459382842588084	32.0	32.0	32.0	32.0	32.0
5	31.376898253929877	32.0	32.0	32.0	32.0	32.0
6	34.89852975061492	36.0	36.0	36.0	36.0	36.0
7	34.93216455714881	36.0	36.0	36.0	36.0	36.0
8	34.86311289240608	36.0	36.0	36.0	32.0	36.0
9	34.9727766067693	36.0	36.0	36.0	36.0	36.0
10	34.8171257436093	36.0	36.0	36.0	32.0	36.0
11	34.990487022237055	36.0	36.0	36.0	36.0	36.0
12	34.89657289896675	36.0	36.0	36.0	32.0	36.0
13	34.94937497658929	36.0	36.0	36.0	36.0	36.0
14	34.890939572557315	36.0	36.0	36.0	32.0	36.0
15	34.864534561019376	36.0	36.0	36.0	32.0	36.0
16	34.87714342791828	36.0	36.0	36.0	32.0	36.0
17	34.835937171895345	36.0	36.0	36.0	32.0	36.0
18	34.84596830672174	36.0	36.0	36.0	32.0	36.0
19	34.83589120177252	36.0	36.0	36.0	32.0	36.0
20	34.82414782175397	36.0	36.0	36.0	32.0	36.0
21	34.81670917484197	36.0	36.0	36.0	32.0	36.0
22	34.80352312751204	36.0	36.0	36.0	32.0	36.0
23	34.743323832841284	36.0	36.0	36.0	32.0	36.0
24	34.72172809069622	36.0	36.0	36.0	32.0	36.0
25	34.705313919185656	36.0	36.0	36.0	32.0	36.0
26	34.63495749750002	36.0	36.0	36.0	32.0	36.0
27	34.62886049713566	36.0	36.0	36.0	32.0	36.0
28	34.60006163401653	36.0	36.0	36.0	32.0	36.0
29	34.56319132538107	36.0	36.0	36.0	32.0	36.0
30	34.53742932803031	36.0	36.0	36.0	32.0	36.0
31	34.53529937900607	36.0	36.0	36.0	32.0	36.0
32	34.50852206622649	36.0	36.0	36.0	32.0	36.0
33	34.47776805405633	36.0	36.0	36.0	32.0	36.0
34	34.46975449684282	36.0	36.0	36.0	32.0	36.0
35	34.43356465953165	36.0	36.0	36.0	32.0	36.0
36	34.40986677744899	36.0	36.0	36.0	32.0	36.0
37	34.40716532331752	36.0	36.0	36.0	32.0	36.0
38	33.967778349093706	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	8.0
21	40.0
22	160.0
23	422.0
24	1190.0
25	2912.0
26	6316.0
27	11946.0
28	20776.0
29	32652.0
30	49269.0
31	71141.0
32	103093.0
33	165206.0
34	402897.0
35	893982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14598565832207	18.178423005713288	11.914354051234548	26.7612372847301
2	16.11962220504491	20.30034948643995	36.27689677834569	27.303131530169455
3	17.94440906825939	24.087833581344984	28.560840038728408	29.40691731166722
4	12.068526168649154	15.766739518947931	35.6756732214802	36.48906109092272
5	14.394226600045856	36.78675287114957	32.98797057000747	15.831049958797102
6	35.04100430758055	35.53368028558294	16.1356632482222	13.28965215861431
7	30.684938970301545	30.314850474826777	20.432335552956253	18.567875001915425
8	28.626164537888098	32.63021683177589	18.864960427704297	19.878658202631723
9	27.40637530470415	13.76113464872826	18.22912616951057	40.60336387705702
10	15.773663247434442	26.535277645667353	31.10827846178818	26.582780645110027
11	38.33985364904034	20.7980571294303	21.612164848867348	19.249924372662008
12	24.816317311064775	23.536582735813614	28.26165177644117	23.38544817668044
13	29.64147215880037	19.046362366636757	25.086108997049394	26.226056477513477
14	23.793341029072415	19.37544196584136	24.70337920130033	32.127837803785894
15	25.37482676074083	26.997118070571517	21.949995857013622	25.67805931167403
16	26.22010948834686	25.563701536991196	23.199134626626122	25.017054348035828
17	24.366094707533538	25.816196693102324	24.64855557333824	25.1691530260259
18	25.382942473782844	24.388568989803712	25.77720721855786	24.451281317855592
19	25.774142543702833	24.725739977094392	25.095771089219493	24.40434638998328
20	26.09144989767391	23.844929722465317	24.55536675645029	25.508253623410482
21	27.317294093343293	23.93530804557517	24.13893889485429	24.608458966227246
22	26.16520205310747	23.865898756648647	24.8104439697346	25.15845522050928
23	24.516007836496005	23.661700374344782	25.445002644703894	26.377289144455318
24	25.06977810618985	24.672618946273982	24.94015371046995	25.31744923706622
25	25.12370794086082	24.262931090746775	25.03630790465224	25.577053063740163
26	25.143500562424588	25.06977810618985	25.352182218756493	24.43453911262907
27	25.786033239274193	24.238370680789913	24.51873199501479	25.4568640849211
28	24.72874789871136	24.333404842413284	25.52806050349203	25.40978675538333
29	24.764729451638864	24.55746662625836	25.437709348506882	25.240094573595893
30	24.649577131623246	24.772618151728647	25.625959839138623	24.951844877509487
31	25.435098699556303	24.588283634522767	24.40678677921969	25.56983088670124
32	25.105702905879294	24.511496503433	24.427161191681794	25.955639399005907
33	24.58317584309773	24.080625920111874	25.18203601106461	26.15416222572579
34	25.580670755169937	24.178127983092075	25.286234956135424	24.954966305602568
35	26.115059244705208	24.10588111104679	25.125680045675008	24.653379598572997
36	24.806613341324187	24.938224100376047	24.766659061732767	25.488503496566995
37	26.039350425138508	24.6709163491323	24.521882345997252	24.76785087973194
38	24.948695014562897	24.329800251076612	25.20970345264391	25.511801281716583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	50.0
2	77.0
3	77.0
4	325.5
5	574.0
6	574.0
7	814.0
8	1054.0
9	1101.0
10	1148.0
11	1148.0
12	1538.5
13	1929.0
14	2721.5
15	3514.0
16	3514.0
17	5276.0
18	7038.0
19	7038.0
20	8494.5
21	9951.0
22	10508.5
23	11066.0
24	11066.0
25	13101.5
26	15137.0
27	15137.0
28	19564.0
29	23991.0
30	29798.0
31	35605.0
32	35605.0
33	46262.0
34	56919.0
35	56919.0
36	64147.0
37	71375.0
38	82325.5
39	93276.0
40	93276.0
41	100136.0
42	106996.0
43	122720.0
44	138444.0
45	138444.0
46	141726.0
47	145008.0
48	145008.0
49	154627.5
50	164247.0
51	166685.5
52	169124.0
53	169124.0
54	161598.0
55	154072.0
56	154072.0
57	149646.0
58	145220.0
59	130248.0
60	115276.0
61	115276.0
62	109292.0
63	103308.0
64	86096.5
65	68885.0
66	68885.0
67	57745.5
68	46606.0
69	46606.0
70	37750.0
71	28894.0
72	22720.5
73	16547.0
74	16547.0
75	12595.0
76	8643.0
77	8643.0
78	8141.5
79	7640.0
80	6111.5
81	4583.0
82	4583.0
83	4194.0
84	3805.0
85	3805.0
86	2454.5
87	1104.0
88	917.5
89	731.0
90	731.0
91	431.0
92	131.0
93	88.5
94	46.0
95	46.0
96	28.5
97	11.0
98	11.0
99	13.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008853505136735577
2	0.0
3	0.0
4	5.675323805599728E-5
5	1.1350647611199456E-4
6	2.2701295222398913E-4
7	1.7025971416799186E-4
8	0.002780908664743867
9	0.00334844104530384
10	0.0011350647611199458
11	0.004824025234759769
12	4.5402590444797825E-4
13	1.7025971416799186E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1350647611199456E-4
22	1.1350647611199456E-4
23	1.1350647611199456E-4
24	0.0
25	5.675323805599728E-5
26	0.0
27	1.1350647611199456E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.1350647611199456E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1762014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.394912852863285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.69217386074142	40.18279765930187
2	14.168069773019615	14.846695621012984
3	4.307795208820688	6.771196636624258
4	1.769816874486669	3.7091760361702355
5	0.8997308786600403	2.3570660489211464
6	0.5056756477008918	1.589689889586205
7	0.31471548363545154	1.1542643236968342
8	0.23344176294552635	0.9784928660599699
9	0.1621930203154762	0.7648280248294854
>10	0.7891345697773096	7.383864630169594
>50	0.06711202751739452	2.4857477474073724
>100	0.07150284300822785	8.293301802173678
>500	0.01318832733292838	4.827985837310454
>1k	0.005449722038400157	4.65489287673597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4456	0.2528924287775239	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3128	0.1775241286391595	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2572	0.145969328280025	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2395	0.1359240051441135	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2285	0.1296811489579538	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2268	0.12871634391100184	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2216	0.12576517553209	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2213	0.12559491581792198	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2157	0.12241673448678614	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2037	0.11560634592006647	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1969	0.11174712573225865	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1909	0.10834193144889882	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1831	0.10391517888053102	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1807	0.1025531011671871	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1789	0.10153154288217914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.675323805599728E-5
7	0.0	0.0	0.0	0.0	5.675323805599728E-5
8	0.0	0.0	0.0	0.0	5.675323805599728E-5
9	0.0	0.0	0.0	0.0	5.675323805599728E-5
10	0.0	0.0	5.675323805599728E-5	0.0	5.675323805599728E-5
11	0.0	0.0	5.675323805599728E-5	5.675323805599728E-5	5.675323805599728E-5
12	0.0	0.0	5.675323805599728E-5	5.675323805599728E-5	2.2701295222398913E-4
13	0.0	0.0	5.675323805599728E-5	5.675323805599728E-5	2.2701295222398913E-4
14	0.0	0.0	5.675323805599728E-5	5.675323805599728E-5	2.2701295222398913E-4
15	0.0	0.0	5.675323805599728E-5	1.1350647611199456E-4	3.405194283359837E-4
16	0.0	0.0	5.675323805599728E-5	1.1350647611199456E-4	3.97272666391981E-4
17	0.0	0.0	5.675323805599728E-5	1.1350647611199456E-4	3.97272666391981E-4
18	0.0	0.0	5.675323805599728E-5	1.1350647611199456E-4	4.5402590444797825E-4
19	0.0	0.0	5.675323805599728E-5	1.7025971416799186E-4	4.5402590444797825E-4
20	0.0	0.0	5.675323805599728E-5	1.7025971416799186E-4	5.107791425039756E-4
21	0.0	0.0	5.675323805599728E-5	1.7025971416799186E-4	5.107791425039756E-4
22	0.0	0.0	5.675323805599728E-5	3.405194283359837E-4	5.107791425039756E-4
23	0.0	0.0	1.7025971416799186E-4	9.648050469519538E-4	6.810388566719674E-4
24	0.0	0.0	1.7025971416799186E-4	0.0014755841894559295	6.810388566719674E-4
25	0.0	0.0	1.7025971416799186E-4	0.001816103617791913	6.810388566719674E-4
26	0.0	0.0	1.7025971416799186E-4	0.002383635998351886	6.810388566719674E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	250	0.0	18.560972	10
TCGCGTA	255	0.0	18.197548	9
CTAGCGG	475	0.0	18.188877	29
TCTAGCG	480	0.0	17.999409	28
GCGTAAC	270	0.0	17.778708	11
CAAGACG	515	0.0	17.709162	4
TAGCGGC	490	0.0	17.632074	30
ATCGTTT	405	0.0	16.592049	29
CGGTCCA	570	0.0	16.562271	10
AAGACGG	580	0.0	16.55353	5
ACCGTCC	120	3.1088348E-8	16.001745	8
CGCAAGA	540	0.0	15.703634	2
GTATAGG	195	0.0	15.590117	1
GTATCAA	3025	0.0	15.497892	1
AGCGGCG	620	0.0	15.483362	31
CATCGTT	435	0.0	15.447769	28
TAATACT	270	0.0	15.407777	4
CGTTTAT	445	0.0	15.100628	31
CGCCGGT	610	0.0	14.952452	7
CCCCTAT	75	6.245558E-4	14.933691	1
>>END_MODULE