FastQCFastQC Report
Thu 2 Feb 2017
SRR4062859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734197
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42840.24703075832791777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26730.154134737864268No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18000.10379443627223435No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17840.10287181906092562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3950.015.79631529
TCTAGCG3900.015.58815728
ATCGTTT3700.015.1340329
CGTTATT1807.2759576E-1215.1182912
TCACGTT1308.324605E-814.76815327
CATCGTT3800.014.73534128
TATACTG2200.014.5422965
GTCTTAA2250.014.2400731
TAGCGGC4400.014.18078330
ACCGTCG2850.014.0336588
GTATCAA40950.013.8879831
GTATAGA1751.1496013E-913.7314991
TTATCCG1053.687421E-513.71328527
TCGTTTA4150.013.4929930
CGTTTAT4150.013.4929931
TAGACTA952.4325556E-413.47075755
ATAGCGT3800.013.47075756
CGGTCCA4650.013.41914910
GTTAGAC1453.1557283E-713.2407953
AGCGGCG4850.013.19491331
Produced by FastQC (version 0.11.5)