##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062859_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1734197
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277684715173653	32.0	32.0	32.0	32.0	32.0
2	30.949581852580764	32.0	32.0	32.0	32.0	32.0
3	30.994791249206404	32.0	32.0	32.0	32.0	32.0
4	31.056716739793693	32.0	32.0	32.0	32.0	32.0
5	30.994474099540017	32.0	32.0	32.0	32.0	32.0
6	34.63861199160188	36.0	36.0	36.0	32.0	36.0
7	34.571149067839464	36.0	36.0	36.0	32.0	36.0
8	34.57043865258676	36.0	36.0	36.0	32.0	36.0
9	34.686412212684026	36.0	36.0	36.0	32.0	36.0
10	34.45229867195019	36.0	36.0	36.0	32.0	36.0
11	34.67165379711763	36.0	36.0	36.0	32.0	36.0
12	34.517233047917856	36.0	36.0	36.0	32.0	36.0
13	34.583584794576396	36.0	36.0	36.0	32.0	36.0
14	34.499788086359274	36.0	36.0	36.0	32.0	36.0
15	34.457149331938645	36.0	36.0	36.0	32.0	36.0
16	34.46888271632346	36.0	36.0	36.0	32.0	36.0
17	34.404131133890786	36.0	36.0	36.0	32.0	36.0
18	34.42498285950212	36.0	36.0	36.0	32.0	36.0
19	34.41423436899037	36.0	36.0	36.0	32.0	36.0
20	34.3946523953161	36.0	36.0	36.0	32.0	36.0
21	34.381039178363245	36.0	36.0	36.0	32.0	36.0
22	34.35630438756381	36.0	36.0	36.0	32.0	36.0
23	34.31074900948393	36.0	36.0	36.0	32.0	36.0
24	34.29504318136867	36.0	36.0	36.0	32.0	36.0
25	34.26528358658215	36.0	36.0	36.0	32.0	36.0
26	34.225559149277736	36.0	36.0	36.0	32.0	36.0
27	34.22536366975609	36.0	36.0	36.0	32.0	36.0
28	34.21667088571829	36.0	36.0	36.0	32.0	36.0
29	34.18201622999002	36.0	36.0	36.0	32.0	36.0
30	34.1529953056083	36.0	36.0	36.0	32.0	36.0
31	34.16404018689918	36.0	36.0	36.0	32.0	36.0
32	34.127546639741624	36.0	36.0	36.0	32.0	36.0
33	34.09985601405146	36.0	36.0	36.0	32.0	36.0
34	34.09583743946045	36.0	36.0	36.0	32.0	36.0
35	34.056272153624995	36.0	36.0	36.0	32.0	36.0
36	34.01422445085535	36.0	36.0	36.0	32.0	36.0
37	34.01157769273041	36.0	36.0	36.0	32.0	36.0
38	33.43489868798066	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	4.0
9	2.0
10	20.0
11	16.0
12	13.0
13	7.0
14	252.0
15	504.0
16	659.0
17	758.0
18	938.0
19	1210.0
20	1482.0
21	2104.0
22	3029.0
23	4439.0
24	6557.0
25	9705.0
26	14721.0
27	21355.0
28	29985.0
29	41024.0
30	55400.0
31	76022.0
32	105801.0
33	160038.0
34	368481.0
35	829667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99040457809011	17.460203824774432	11.650239365940063	25.899152231195398
2	16.47389856207129	19.93830112903049	37.821800229279326	25.76600007961889
3	18.419802098964357	24.40066251518168	28.8249467415764	28.354588644277563
4	12.547084074338056	15.683696512145556	36.53187877278085	35.23734064073554
5	14.460517901430697	36.65651592010338	33.83756460505216	15.04540157341377
6	33.453447115625806	35.94602433760103	17.191494122036293	13.409034424736866
7	29.741719078051688	30.85848954876522	21.35426367361955	18.045527699563543
8	27.90759495349705	32.90548734251303	19.83593502676464	19.35098267722529
9	27.726178428608083	14.299891304034437	18.736935581062106	39.23699468629537
10	15.692868539703571	26.745297081229324	31.990861434364998	25.57097294470211
11	36.866306299656664	21.457041399918577	22.655086791399633	19.02156550902513
12	24.79301098520889	23.8810944843295	28.95320474007439	22.37268979038722
13	29.32926934766686	19.738916795949088	25.33112640071975	25.6006874556643
14	23.724017263770765	19.851202627857784	25.496125801309777	30.928654307061677
15	25.090149846001225	27.02661237296543	22.77884160620148	25.104396174831873
16	25.52651718090484	25.940533648137333	23.91174338476196	24.621205786195876
17	23.9168086928687	25.896408827829635	25.508713343541917	24.67806913575975
18	24.843434273535028	24.919727627536194	26.34237089526762	23.894467203661158
19	25.38153411777732	25.349989504351655	25.72270035731339	23.54577602055763
20	25.632453884742272	24.419129693629877	25.42335153127395	24.5250648903539
21	26.710962918708574	24.452015533290812	24.660713853208787	24.176307694791827
22	25.661481538722374	24.493876228185613	25.129089963334643	24.715552269757367
23	24.15095036005783	24.415306105383596	25.680626656909034	25.75311687764954
24	24.514906758864903	25.348727900827306	25.476586627603524	24.659778712704266
25	24.718625479338762	24.66291289169669	25.48470239616033	25.13375923280422
26	24.504531211520174	25.40408325225234	25.832433127646187	24.2589524085813
27	25.21922868871083	25.10048822572106	24.928115596349322	24.75216748921878
28	24.40488993945531	24.855212327380745	25.868538617638865	24.87135911552508
29	24.397008108326318	25.248670718907512	25.725078142120623	24.62924303064555
30	24.475978541487784	25.249273846299342	25.907584002878664	24.36716360933421
31	24.873305453228546	25.001557010816033	24.951098326963045	25.174039208992372
32	24.46917896103881	25.193433441422698	24.93525306809053	25.402134529447963
33	24.278603694393357	24.752239432582886	25.55776043477835	25.411396438245408
34	24.856658419695844	24.93434142459212	25.69808012826058	24.510920027451455
35	25.5608422582487	24.842004486968758	25.43448045169588	24.162672803086664
36	24.511517122853157	25.434732459023955	25.23472553836926	24.819024879753623
37	25.35952687837187	25.256932610156195	24.983866921566424	24.399673589905507
38	24.45403767039157	25.156710074540367	25.53489026546427	24.85436198960379
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	463.0
1	404.5
2	346.0
3	346.0
4	724.5
5	1103.0
6	1103.0
7	1287.5
8	1472.0
9	1416.5
10	1361.0
11	1361.0
12	1824.0
13	2287.0
14	3061.5
15	3836.0
16	3836.0
17	5682.5
18	7529.0
19	7529.0
20	9520.5
21	11512.0
22	12818.0
23	14124.0
24	14124.0
25	16719.5
26	19315.0
27	19315.0
28	24388.0
29	29461.0
30	35806.5
31	42152.0
32	42152.0
33	52340.0
34	62528.0
35	62528.0
36	69569.5
37	76611.0
38	87025.5
39	97440.0
40	97440.0
41	105246.0
42	113052.0
43	126408.0
44	139764.0
45	139764.0
46	144794.5
47	149825.0
48	149825.0
49	156544.0
50	163263.0
51	162107.5
52	160952.0
53	160952.0
54	153349.5
55	145747.0
56	145747.0
57	139850.0
58	133953.0
59	119804.0
60	105655.0
61	105655.0
62	98465.0
63	91275.0
64	75009.0
65	58743.0
66	58743.0
67	48741.5
68	38740.0
69	38740.0
70	31178.0
71	23616.0
72	18526.5
73	13437.0
74	13437.0
75	10402.0
76	7367.0
77	7367.0
78	6884.0
79	6401.0
80	5005.5
81	3610.0
82	3610.0
83	3626.0
84	3642.0
85	3642.0
86	2421.0
87	1200.0
88	999.5
89	799.0
90	799.0
91	592.0
92	385.0
93	318.5
94	252.0
95	252.0
96	235.5
97	219.0
98	219.0
99	489.5
100	760.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12230444407411614
2	0.05420376116438905
3	0.011475051565652577
4	0.003748132420941796
5	3.459814542407812E-4
6	4.6130860565437486E-4
7	0.0
8	3.459814542407812E-4
9	1.1532715141359371E-4
10	4.6130860565437486E-4
11	0.0022488794525650775
12	0.0027101880582194524
13	0.015396174713714763
14	0.009283835688794295
15	0.024045711069734292
16	0.011994023747013747
17	0.020874214405860462
18	0.005824021146386483
19	0.008361218477485544
20	0.006804301933402031
21	0.006631311206281639
22	0.007784582720417576
23	0.009802807870155467
24	0.01453122107811281
25	0.017183745560625463
26	0.01925963428607015
27	0.009399162840207888
28	0.006112339024920467
29	0.010206452900103044
30	0.0034598145424078117
31	0.00605467544921367
32	0.007957573447537968
33	0.009456826415914686
34	0.013089631685442887
35	0.0165494462278507
36	0.014819538956646795
37	0.01049477077863703
38	0.005939348297800077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1734197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.60538220063805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75358923300537	46.518413718406045
2	11.873019832458736	13.67899690649082
3	3.321096549953835	5.739391082559332
4	1.3410249146866278	3.0900101100440494
5	0.6931053795773999	1.9963300147937215
6	0.4405656226538359	1.522737064646178
7	0.2941633798783323	1.186177574912597
8	0.20491103648899103	0.9443182859261774
9	0.16248648827223747	0.8424086633425557
>10	0.7767908865685373	7.946048210919998
>50	0.0620580019357688	2.475643018634762
>100	0.06601844961597986	8.117154367670242
>500	0.007547449259733978	2.9001724532830777
>1k	0.0036227756446723094	3.042198528370398
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4284	0.24703075832791777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2673	0.154134737864268	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1800	0.10379443627223435	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1784	0.10287181906092562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.7663575706796864E-5	0.0
4	0.0	0.0	0.0	5.7663575706796864E-5	0.0
5	0.0	0.0	0.0	5.7663575706796864E-5	0.0
6	0.0	0.0	0.0	5.7663575706796864E-5	5.7663575706796864E-5
7	0.0	0.0	0.0	5.7663575706796864E-5	5.7663575706796864E-5
8	0.0	0.0	0.0	5.7663575706796864E-5	5.7663575706796864E-5
9	0.0	0.0	0.0	1.1532715141359373E-4	5.7663575706796864E-5
10	0.0	0.0	0.0	1.1532715141359373E-4	5.7663575706796864E-5
11	0.0	0.0	0.0	1.1532715141359373E-4	5.7663575706796864E-5
12	0.0	0.0	0.0	1.1532715141359373E-4	5.7663575706796864E-5
13	0.0	0.0	0.0	1.1532715141359373E-4	5.7663575706796864E-5
14	0.0	0.0	0.0	1.1532715141359373E-4	5.7663575706796864E-5
15	0.0	0.0	0.0	1.1532715141359373E-4	1.1532715141359373E-4
16	0.0	0.0	0.0	2.8831787853398434E-4	1.1532715141359373E-4
17	0.0	0.0	0.0	2.8831787853398434E-4	1.1532715141359373E-4
18	0.0	0.0	0.0	4.0364502994757806E-4	1.1532715141359373E-4
19	0.0	0.0	0.0	4.0364502994757806E-4	1.1532715141359373E-4
20	0.0	0.0	0.0	5.189721813611718E-4	1.1532715141359373E-4
21	0.0	0.0	0.0	5.766357570679687E-4	1.1532715141359373E-4
22	0.0	0.0	0.0	9.226172113087498E-4	1.729907271203906E-4
23	0.0	0.0	0.0	0.0013839258169631248	1.729907271203906E-4
24	0.0	0.0	0.0	0.0019605615740310935	1.729907271203906E-4
25	0.0	0.0	0.0	0.0024218701796854683	1.729907271203906E-4
26	0.0	0.0	0.0	0.0031138330881670304	2.3065430282718746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	395	0.0	15.796315	29
TCTAGCG	390	0.0	15.588157	28
ATCGTTT	370	0.0	15.13403	29
CGTTATT	180	7.2759576E-12	15.118291	2
TCACGTT	130	8.324605E-8	14.768153	27
CATCGTT	380	0.0	14.735341	28
TATACTG	220	0.0	14.542296	5
GTCTTAA	225	0.0	14.240073	1
TAGCGGC	440	0.0	14.180783	30
ACCGTCG	285	0.0	14.033658	8
GTATCAA	4095	0.0	13.887983	1
GTATAGA	175	1.1496013E-9	13.731499	1
TTATCCG	105	3.687421E-5	13.713285	27
TCGTTTA	415	0.0	13.49299	30
CGTTTAT	415	0.0	13.49299	31
TAGACTA	95	2.4325556E-4	13.4707575	5
ATAGCGT	380	0.0	13.4707575	6
CGGTCCA	465	0.0	13.419149	10
GTTAGAC	145	3.1557283E-7	13.240795	3
AGCGGCG	485	0.0	13.194913	31
>>END_MODULE