FastQCFastQC Report
Thu 2 Feb 2017
SRR4062859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734197
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49140.28335881102319976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34670.19991961697546473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19490.1123863090525471No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19400.11186733687118591No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18280.10540901639202467No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17580.10137256609254888No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17490.10085359391118771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3600.021.3324728
TAGTACG604.7245103E-621.3324682
CTAGCGG3950.019.4422529
TAGCGGC4150.019.27632730
TATACGG450.00884675417.7796215
ACCGTCG3050.017.3132698
CATCGTT4300.017.11558528
ATCGTTT4550.016.17516729
CGTTTAT4550.016.17516731
GGCATCG5050.016.15776326
CCGTCGT3300.016.0016579
CGCGGAA600.002442768815.99981325
CGGCATC4650.015.82777125
ATACCGT3900.015.591366
TACCGTC3600.015.5571687
CGTCGTA3400.015.52967610
TCGTTTA4650.015.48324230
TGCGTAG859.4398594E-515.05821213
CGCATCG4800.014.999392513
ACCGTTC756.2417146E-414.934888
Produced by FastQC (version 0.11.5)