##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1734197
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20822144196997	32.0	32.0	32.0	32.0	32.0
2	31.292523859746037	32.0	32.0	32.0	32.0	32.0
3	31.37362537243462	32.0	32.0	32.0	32.0	32.0
4	31.473269761163237	32.0	32.0	32.0	32.0	32.0
5	31.398689422251337	32.0	32.0	32.0	32.0	32.0
6	34.92695293556614	36.0	36.0	36.0	36.0	36.0
7	34.95723784552735	36.0	36.0	36.0	36.0	36.0
8	34.89349652894106	36.0	36.0	36.0	32.0	36.0
9	35.00956004421643	36.0	36.0	36.0	36.0	36.0
10	34.85035840795481	36.0	36.0	36.0	32.0	36.0
11	35.02596187169047	36.0	36.0	36.0	36.0	36.0
12	34.92681281307718	36.0	36.0	36.0	32.0	36.0
13	34.973354238301646	36.0	36.0	36.0	36.0	36.0
14	34.93163983099959	36.0	36.0	36.0	32.0	36.0
15	34.896486385341454	36.0	36.0	36.0	32.0	36.0
16	34.911602891712995	36.0	36.0	36.0	32.0	36.0
17	34.88472359253303	36.0	36.0	36.0	32.0	36.0
18	34.877460288537	36.0	36.0	36.0	32.0	36.0
19	34.86742278991372	36.0	36.0	36.0	32.0	36.0
20	34.855576961556274	36.0	36.0	36.0	32.0	36.0
21	34.85485616685994	36.0	36.0	36.0	32.0	36.0
22	34.82922701400129	36.0	36.0	36.0	32.0	36.0
23	34.77414676648616	36.0	36.0	36.0	32.0	36.0
24	34.749932101139606	36.0	36.0	36.0	32.0	36.0
25	34.740070476422225	36.0	36.0	36.0	32.0	36.0
26	34.67669935999197	36.0	36.0	36.0	32.0	36.0
27	34.660354619457884	36.0	36.0	36.0	32.0	36.0
28	34.622289163226554	36.0	36.0	36.0	32.0	36.0
29	34.598730709371544	36.0	36.0	36.0	32.0	36.0
30	34.57315749018134	36.0	36.0	36.0	32.0	36.0
31	34.56793547676533	36.0	36.0	36.0	32.0	36.0
32	34.53912214125616	36.0	36.0	36.0	32.0	36.0
33	34.506632752795674	36.0	36.0	36.0	32.0	36.0
34	34.496723267310465	36.0	36.0	36.0	32.0	36.0
35	34.46518359794187	36.0	36.0	36.0	32.0	36.0
36	34.43388207914095	36.0	36.0	36.0	32.0	36.0
37	34.427563304515	36.0	36.0	36.0	32.0	36.0
38	33.99867258448723	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	11.0
21	40.0
22	108.0
23	403.0
24	1038.0
25	2679.0
26	5836.0
27	11176.0
28	19317.0
29	31173.0
30	47698.0
31	68408.0
32	99117.0
33	159855.0
34	393007.0
35	894328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33140297316219	18.68756653514382	12.39125144315664	26.58977904853735
2	15.489993351389723	20.910196477101508	37.16088771921529	26.438922452293482
3	17.42650921435108	25.14333723331317	29.227417646322767	28.202735906012983
4	11.98930916073452	16.627426558143693	36.52645752063638	34.85680676048541
5	13.943019062457834	37.18839991373514	33.56429557477421	15.304285449032806
6	33.544440292655025	36.38305331820237	16.84513594803805	13.227370441104561
7	29.473882088095156	31.027803710429	21.408862796701406	18.08945140477444
8	27.75690306985069	33.244875316868836	19.610000484385488	19.388221128894976
9	27.559662654076263	14.189288546096734	18.541858285879044	39.70919051394796
10	15.48878319716939	27.044246746596674	31.981900421177915	25.48506963505603
11	36.95126782541602	21.4071071575003	22.575832804243788	19.065792212839895
12	24.48435670078244	24.066179485213006	28.90537558391713	22.544088230087425
13	29.39709778888764	19.664455265987964	25.29029319078881	25.648153754335585
14	23.582845547535833	19.786102732273207	25.547039926836458	31.084011793354506
15	24.99122072059864	27.2076932436165	22.65774880247169	25.14333723331317
16	25.4557584864926	26.01624844236266	23.801736480918834	24.72625659022591
17	23.86810725655736	25.995777872986746	25.46608026654411	24.67003460391178
18	24.825553267593012	24.94820369312137	26.375665509743122	23.85057752954249
19	25.426061745003597	25.27959626270833	25.773138807182804	23.521203185105268
20	25.64725922141487	24.33362530323833	25.415624637800665	24.60349083754614
21	26.621846665544147	24.462229182860533	24.720158505728303	24.195765645867017
22	25.552547435553674	24.530517041047865	25.181827879794373	24.735107643604092
23	24.105291443412394	24.336061206461093	25.664515429628487	25.894131920498033
24	24.534409873849395	25.294588792392098	25.446589977955213	24.72441135580329
25	24.639803851241556	24.566628647083313	25.608784253664812	25.184783248010316
26	24.566888633118747	25.254584833548225	25.919273254003585	24.25925327932944
27	25.292815806287177	24.943547326830416	24.91067906972453	24.852957797157877
28	24.366608868542617	24.702037888429054	25.95939215671576	24.971961086312568
29	24.528297534824475	25.097552354201973	25.7145526142647	24.65959749670885
30	24.57362110533002	25.013997833002826	25.96931029173733	24.44307076992983
31	24.900400588860435	24.833799158919085	25.040926722857897	25.22487352936258
32	24.477784242505322	25.120502457333277	25.06739430410732	25.334318996054083
33	24.309348937865767	24.598243452156822	25.658849600131933	25.43355800984548
34	24.87099216524997	24.77463633024391	25.922199150384877	24.432172354121246
35	25.6628283868557	24.64091449817985	25.416950900041925	24.279306214922524
36	24.60868055935975	25.2801728984654	25.30496823601932	24.80617830615553
37	25.4362681979037	25.068951220651403	25.075755522584803	24.419025058860093
38	24.59667142199777	24.875936602248654	25.576550259082893	24.950841716670684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	244.0
1	225.0
2	206.0
3	206.0
4	658.5
5	1111.0
6	1111.0
7	1291.0
8	1471.0
9	1444.5
10	1418.0
11	1418.0
12	1827.5
13	2237.0
14	3066.5
15	3896.0
16	3896.0
17	6092.0
18	8288.0
19	8288.0
20	10022.5
21	11757.0
22	13086.0
23	14415.0
24	14415.0
25	16874.0
26	19333.0
27	19333.0
28	24416.5
29	29500.0
30	35811.0
31	42122.0
32	42122.0
33	52766.5
34	63411.0
35	63411.0
36	70435.0
37	77459.0
38	87369.0
39	97279.0
40	97279.0
41	104867.0
42	112455.0
43	125616.0
44	138777.0
45	138777.0
46	144720.5
47	150664.0
48	150664.0
49	157237.5
50	163811.0
51	161911.0
52	160011.0
53	160011.0
54	153305.0
55	146599.0
56	146599.0
57	140532.0
58	134465.0
59	119963.0
60	105461.0
61	105461.0
62	98507.0
63	91553.0
64	75085.5
65	58618.0
66	58618.0
67	48729.5
68	38841.0
69	38841.0
70	31415.0
71	23989.0
72	18807.0
73	13625.0
74	13625.0
75	10315.5
76	7006.0
77	7006.0
78	6652.0
79	6298.0
80	4834.5
81	3371.0
82	3371.0
83	3190.0
84	3009.0
85	3009.0
86	1901.0
87	793.0
88	666.0
89	539.0
90	539.0
91	319.0
92	99.0
93	68.0
94	37.0
95	37.0
96	28.0
97	19.0
98	19.0
99	14.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008707199931726327
2	0.0
3	0.0
4	1.1532715141359371E-4
5	1.729907271203906E-4
6	2.883178785339843E-4
7	2.3065430282718743E-4
8	0.0023642066039786715
9	0.004151777450889374
10	7.496264841883592E-4
11	0.00495906751078453
12	2.3065430282718743E-4
13	1.1532715141359371E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	5.766357570679686E-5
22	1.1532715141359371E-4
23	5.766357570679686E-5
24	0.0
25	1.729907271203906E-4
26	5.766357570679686E-5
27	5.766357570679686E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.729907271203906E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1734197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.81235635453482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3254248213517	44.273388781041426
2	12.747638349307339	14.229514684605505
3	3.5700261745973947	5.977547191549395
4	1.4628031207401824	3.265699562051067
5	0.7545891152978432	2.1057698302128194
6	0.4703604722282348	1.5751155774653711
7	0.29056675474447125	1.1352050682405406
8	0.2156525633252524	0.9628862170462297
9	0.16713323859871967	0.8395289884223313
>10	0.8485633918257983	8.317072171908325
>50	0.06569485535925043	2.557681117161025
>100	0.06877016245380835	8.156581233585573
>500	0.009349009880484115	3.537332200581509
>1k	0.0034279702895108426	3.0666773761290043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4914	0.28335881102319976	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3467	0.19991961697546473	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1949	0.1123863090525471	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1940	0.11186733687118591	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1828	0.10540901639202467	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1758	0.10137256609254888	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1749	0.10085359391118771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.7663575706796864E-5	0.0
4	0.0	0.0	0.0	5.7663575706796864E-5	0.0
5	0.0	0.0	0.0	5.7663575706796864E-5	0.0
6	0.0	0.0	0.0	5.7663575706796864E-5	0.0
7	0.0	0.0	0.0	5.7663575706796864E-5	0.0
8	0.0	0.0	0.0	5.7663575706796864E-5	0.0
9	0.0	0.0	0.0	5.7663575706796864E-5	0.0
10	0.0	0.0	0.0	5.7663575706796864E-5	0.0
11	0.0	0.0	0.0	5.7663575706796864E-5	0.0
12	0.0	0.0	0.0	5.7663575706796864E-5	1.1532715141359373E-4
13	0.0	0.0	0.0	5.7663575706796864E-5	1.1532715141359373E-4
14	0.0	0.0	0.0	5.7663575706796864E-5	1.1532715141359373E-4
15	0.0	0.0	0.0	5.7663575706796864E-5	1.729907271203906E-4
16	0.0	0.0	0.0	2.3065430282718746E-4	1.729907271203906E-4
17	0.0	0.0	0.0	2.3065430282718746E-4	1.729907271203906E-4
18	0.0	0.0	0.0	3.459814542407812E-4	1.729907271203906E-4
19	0.0	0.0	0.0	3.459814542407812E-4	1.729907271203906E-4
20	0.0	0.0	0.0	5.189721813611718E-4	1.729907271203906E-4
21	0.0	0.0	0.0	5.766357570679687E-4	1.729907271203906E-4
22	0.0	0.0	0.0	9.226172113087498E-4	1.729907271203906E-4
23	0.0	0.0	0.0	0.0013839258169631248	1.729907271203906E-4
24	0.0	0.0	0.0	0.0018452344226174997	2.3065430282718746E-4
25	0.0	0.0	0.0	0.0023642066039786715	2.3065430282718746E-4
26	0.0	0.0	0.0	0.0031138330881670304	2.3065430282718746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	360	0.0	21.33247	28
TAGTACG	60	4.7245103E-6	21.332468	2
CTAGCGG	395	0.0	19.44225	29
TAGCGGC	415	0.0	19.276327	30
TATACGG	45	0.008846754	17.779621	5
ACCGTCG	305	0.0	17.313269	8
CATCGTT	430	0.0	17.115585	28
ATCGTTT	455	0.0	16.175167	29
CGTTTAT	455	0.0	16.175167	31
GGCATCG	505	0.0	16.157763	26
CCGTCGT	330	0.0	16.001657	9
CGCGGAA	60	0.0024427688	15.999813	25
CGGCATC	465	0.0	15.827771	25
ATACCGT	390	0.0	15.59136	6
TACCGTC	360	0.0	15.557168	7
CGTCGTA	340	0.0	15.529676	10
TCGTTTA	465	0.0	15.483242	30
TGCGTAG	85	9.4398594E-5	15.058212	13
CGCATCG	480	0.0	14.9993925	13
ACCGTTC	75	6.2417146E-4	14.93488	8
>>END_MODULE