FastQCFastQC Report
Thu 2 Feb 2017
SRR4062858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271853
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA69120.5434590318220738No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA64860.5099645949649841No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG58500.4599588159952447No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC54140.42567812475183847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35140.27628979135167353No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA32170.25293803607806875No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA29600.2327312983497307No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA29020.22817102290909405No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA26980.21213143342823423No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA22960.1805240070983046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22850.17965912727335626No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT21990.172897339551033No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19710.15497073954301321No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG15830.12446406935392691No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG15230.11974654303602696No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT15130.11896028864971031No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA14370.11298475531370371No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT14070.11062599215475373No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG14020.1102328649615954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13370.10512221145053711No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA13200.10378557899379881No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC13110.10307795004611381No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT13000.10221307022116549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG1100.024.720015
CGGACCA1250.020.4804279
GACGGAC1250.020.478017
CACGGTT555.8365094E-520.36246128
ATGGGCG951.7316779E-920.2045925
AGACGGA1350.020.1430236
CCGCAGC1203.6379788E-1219.99884430
TAGGACC17050.019.9816154
GCGCAAG1300.019.7128751
GAGCGAA1300.019.69426716
AGCGAAA1300.019.69426717
TTTGCGC659.427002E-619.69426717
ATTTGCG659.427002E-619.69426716
CAGAGCG1300.019.69426714
GCGAAAG1300.019.69194418
ACGGACC1300.019.6903958
CAAGACG1300.019.6857514
CGTATGT1650.019.3928223
CGGTCCA1003.263267E-919.20040110
CGCAGCT1257.2759576E-1219.1988931
Produced by FastQC (version 0.11.5)