##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062858_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1271853
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332381179271504	32.0	32.0	32.0	32.0	32.0
2	31.072650691550045	32.0	32.0	32.0	32.0	32.0
3	31.105362019038363	32.0	32.0	32.0	32.0	32.0
4	31.151434953567747	32.0	32.0	32.0	32.0	32.0
5	31.1500472145759	32.0	32.0	32.0	32.0	32.0
6	34.8154881106543	36.0	36.0	36.0	32.0	36.0
7	34.744060830929364	36.0	36.0	36.0	32.0	36.0
8	34.74101802645431	36.0	36.0	36.0	32.0	36.0
9	34.81597480211943	36.0	36.0	36.0	32.0	36.0
10	34.6337800044502	36.0	36.0	36.0	32.0	36.0
11	34.80493736304432	36.0	36.0	36.0	36.0	36.0
12	34.67960920011983	36.0	36.0	36.0	32.0	36.0
13	34.7251828631139	36.0	36.0	36.0	32.0	36.0
14	34.68049845383075	36.0	36.0	36.0	32.0	36.0
15	34.658519498715656	36.0	36.0	36.0	32.0	36.0
16	34.690593960151055	36.0	36.0	36.0	32.0	36.0
17	34.65679209782891	36.0	36.0	36.0	32.0	36.0
18	34.6846443732098	36.0	36.0	36.0	32.0	36.0
19	34.59760679889893	36.0	36.0	36.0	32.0	36.0
20	34.576582356608824	36.0	36.0	36.0	32.0	36.0
21	34.53264803401022	36.0	36.0	36.0	32.0	36.0
22	34.51169199585172	36.0	36.0	36.0	32.0	36.0
23	34.51400594251065	36.0	36.0	36.0	32.0	36.0
24	34.47707557398536	36.0	36.0	36.0	32.0	36.0
25	34.45143660470196	36.0	36.0	36.0	32.0	36.0
26	34.45571775983545	36.0	36.0	36.0	32.0	36.0
27	34.401772846390266	36.0	36.0	36.0	32.0	36.0
28	34.316432795299455	36.0	36.0	36.0	32.0	36.0
29	34.26656067957539	36.0	36.0	36.0	32.0	36.0
30	34.209837143128965	36.0	36.0	36.0	32.0	36.0
31	34.21108257007689	36.0	36.0	36.0	32.0	36.0
32	34.18804059903149	36.0	36.0	36.0	32.0	36.0
33	34.22481214417075	36.0	36.0	36.0	32.0	36.0
34	34.170068396269066	36.0	36.0	36.0	32.0	36.0
35	34.17597238045592	36.0	36.0	36.0	32.0	36.0
36	34.09552440415677	36.0	36.0	36.0	32.0	36.0
37	34.01628725961255	36.0	36.0	36.0	32.0	36.0
38	33.466987143954526	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	3.0
9	6.0
10	8.0
11	15.0
12	9.0
13	6.0
14	137.0
15	325.0
16	477.0
17	488.0
18	622.0
19	738.0
20	907.0
21	1149.0
22	1638.0
23	2362.0
24	3659.0
25	5875.0
26	8902.0
27	13391.0
28	19583.0
29	27480.0
30	38503.0
31	52743.0
32	72236.0
33	111107.0
34	272348.0
35	637132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54818525912724	21.318796722571058	13.23244616867045	24.90057184963126
2	11.223610766925102	21.34031932594612	48.82256916172022	18.613500745408558
3	17.672125346491832	28.14492696640258	31.0213694536733	23.161578233432284
4	10.09521068775156	18.137679262091616	41.54406590287393	30.22304414728289
5	9.734945367028056	41.42552861931154	37.226373215101454	11.613152798558952
6	27.452966077707398	40.61727502028544	20.368628955661368	11.56112994634579
7	25.231866600830916	33.12806836015511	24.376814283422917	17.26325075559106
8	21.587721174228367	43.80444833030362	21.13059107692114	13.477239418546871
9	26.798936037218258	15.345296493530286	22.33920850667021	35.51655896258125
10	14.806454250678541	30.143004959727765	36.1819531326169	18.8685876569768
11	32.216014165482015	21.40138887141617	29.854288014693857	16.528308948407954
12	25.224305774512445	24.425917961209706	35.172466392464926	15.177309871812916
13	29.698815029288095	24.33180752659677	27.074091927431258	18.895285516683874
14	18.047127668692507	26.420871611032787	28.679907874033706	26.852092846241003
15	18.769916543195894	40.516579686620176	23.1670488755509	17.546454894633033
16	17.05530165540105	28.955678783627793	35.70498444621283	18.284035114758325
17	17.528037577943326	32.96681259864564	31.765016746657942	17.74013307675309
18	17.95226839904449	27.534915684970173	38.65084944070427	15.861966475281065
19	23.27169390863463	26.351306191227685	29.035951220010837	21.341048680126846
20	23.583056356932744	29.22936476671471	31.05894230791913	16.128636568433418
21	20.535934031630156	27.567758407436614	27.742948557158147	24.15335900377508
22	22.362438884005027	33.54039513682592	28.237752032219866	15.859413946949195
23	17.26304681504221	32.99943069587207	31.774640489161204	17.962881999924512
24	22.429761762467052	27.99865059543656	32.8830862046188	16.688501437477587
25	19.511479700165772	30.609466860013402	32.426496261508795	17.452557178312034
26	15.815325478267503	32.688906521675875	33.715312974541945	17.780455025514676
27	17.874214558513277	32.01133567710934	31.42575862776659	18.688691136610792
28	16.683454227359398	32.844724892374835	29.20831121857247	21.263509661693302
29	19.82616942072725	29.945867650967443	28.464226569515716	21.763736358789586
30	17.068050312586244	30.218216729084318	31.620864453703344	21.09286850462609
31	22.120858521365534	27.944798107830987	30.38541620798762	19.54892716281586
32	18.379752926828694	31.92152033899238	30.95609344149763	18.742633292681298
33	16.44842425915136	32.500623156577035	34.05485822303265	16.996094361238953
34	18.609528362866026	31.660504302122046	33.31852373755404	16.411443597457886
35	17.45477536307103	32.65350897887158	32.56480546564626	17.326910192411134
36	16.3217241072896	31.298121118757276	34.072209496084255	18.307945277868868
37	17.307043582535535	31.24755745149305	29.222827262183255	22.22257170378816
38	18.50009278392668	34.57545849413259	28.804219624272275	18.12022909766845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	420.0
1	341.0
2	262.0
3	262.0
4	553.5
5	845.0
6	845.0
7	981.5
8	1118.0
9	1101.0
10	1084.0
11	1084.0
12	1529.5
13	1975.0
14	2774.5
15	3574.0
16	3574.0
17	5475.5
18	7377.0
19	7377.0
20	10094.5
21	12812.0
22	16440.0
23	20068.0
24	20068.0
25	29019.0
26	37970.0
27	37970.0
28	52791.5
29	67613.0
30	92851.0
31	118089.0
32	118089.0
33	145151.5
34	172214.0
35	172214.0
36	178535.0
37	184856.0
38	182145.5
39	179435.0
40	179435.0
41	156301.5
42	133168.0
43	112045.5
44	90923.0
45	90923.0
46	78691.0
47	66459.0
48	66459.0
49	57925.5
50	49392.0
51	42458.0
52	35524.0
53	35524.0
54	29583.0
55	23642.0
56	23642.0
57	20930.0
58	18218.0
59	15162.0
60	12106.0
61	12106.0
62	11702.0
63	11298.0
64	9164.5
65	7031.0
66	7031.0
67	5950.5
68	4870.0
69	4870.0
70	3988.0
71	3106.0
72	2446.0
73	1786.0
74	1786.0
75	1370.0
76	954.0
77	954.0
78	1021.0
79	1088.0
80	883.5
81	679.0
82	679.0
83	735.0
84	791.0
85	791.0
86	527.0
87	263.0
88	235.5
89	208.0
90	208.0
91	142.5
92	77.0
93	70.5
94	64.0
95	64.0
96	61.5
97	59.0
98	59.0
99	247.0
100	435.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1249358219857169
2	0.05802557371016934
3	0.013995328076436504
4	0.0033022684225299625
5	1.5725087726333153E-4
6	3.931271931583288E-4
7	7.862543863166577E-5
8	3.931271931583288E-4
9	3.1450175452666306E-4
10	7.076289476849919E-4
11	0.0022801377203183074
12	0.0031450175452666304
13	0.013838077199173175
14	0.00912055088127323
15	0.02335175527360473
16	0.01077168509253821
17	0.020128112289706436
18	0.005896907897374932
19	0.008019794740429909
20	0.006604536845059925
21	0.007233540354113251
22	0.007154914915481585
23	0.009828179828958221
24	0.014231204392331503
25	0.01706172018307147
26	0.01824110176254646
27	0.00912055088127323
28	0.00613278421326993
29	0.012186942987908194
30	0.003459519299793294
31	0.00668316228369159
32	0.006604536845059925
33	0.008019794740429909
34	0.012186942987908194
35	0.01580371316496482
36	0.015096084217279827
37	0.010064056144853218
38	0.006368660529164927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1271853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.19175919040396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.34249620374521	51.97106739394199
2	8.859971789598447	10.665945767865644
3	1.9468925197642841	3.515606571577517
4	0.7730927840163364	1.861352587494012
5	0.41817132360484927	1.2585233805377787
6	0.2813292220705511	1.0160220472856574
7	0.19553559011556598	0.8238741807372777
8	0.14314507856323685	0.6892923278535846
9	0.11353577794561279	0.6150526385037751
>10	0.7695889695643604	9.038750359111065
>50	0.08616402504416545	3.6692152957808997
>100	0.06207170604453188	7.4948394351701255
>500	0.003674430784227098	1.5669136695332695
>1k	0.003805660455092352	3.8655065910142463
>5k	5.249186834610141E-4	1.9480377535932596
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	6912	0.5434590318220738	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	6486	0.5099645949649841	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	5850	0.4599588159952447	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	5414	0.42567812475183847	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3514	0.27628979135167353	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	3217	0.25293803607806875	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	2960	0.2327312983497307	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	2902	0.22817102290909405	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2698	0.21213143342823423	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	2296	0.1805240070983046	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2285	0.17965912727335626	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	2199	0.172897339551033	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1971	0.15497073954301321	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	1583	0.12446406935392691	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	1523	0.11974654303602696	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	1513	0.11896028864971031	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	1437	0.11298475531370371	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	1407	0.11062599215475373	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	1402	0.1102328649615954	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1337	0.10512221145053711	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	1320	0.10378557899379881	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	1311	0.10307795004611381	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	1300	0.10221307022116549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	7.862543863166577E-5	0.0
14	0.0	0.0	0.0	7.862543863166577E-5	0.0
15	0.0	0.0	0.0	7.862543863166577E-5	0.0
16	0.0	0.0	0.0	7.862543863166577E-5	0.0
17	0.0	0.0	0.0	7.862543863166577E-5	0.0
18	0.0	0.0	0.0	7.862543863166577E-5	0.0
19	0.0	0.0	0.0	7.862543863166577E-5	0.0
20	0.0	0.0	0.0	3.1450175452666306E-4	0.0
21	0.0	0.0	0.0	4.717526317899946E-4	0.0
22	0.0	0.0	0.0	6.290035090533261E-4	0.0
23	0.0	0.0	0.0	0.001022130702211655	0.0
24	0.0	0.0	0.0	0.001965635965791644	0.0
25	0.0	0.0	0.0	0.0027518903521083016	0.0
26	0.0	0.0	0.0	0.003380893861161628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	110	0.0	24.72001	5
CGGACCA	125	0.0	20.480427	9
GACGGAC	125	0.0	20.47801	7
CACGGTT	55	5.8365094E-5	20.362461	28
ATGGGCG	95	1.7316779E-9	20.204592	5
AGACGGA	135	0.0	20.143023	6
CCGCAGC	120	3.6379788E-12	19.998844	30
TAGGACC	1705	0.0	19.981615	4
GCGCAAG	130	0.0	19.712875	1
GAGCGAA	130	0.0	19.694267	16
AGCGAAA	130	0.0	19.694267	17
TTTGCGC	65	9.427002E-6	19.694267	17
ATTTGCG	65	9.427002E-6	19.694267	16
CAGAGCG	130	0.0	19.694267	14
GCGAAAG	130	0.0	19.691944	18
ACGGACC	130	0.0	19.690395	8
CAAGACG	130	0.0	19.685751	4
CGTATGT	165	0.0	19.39282	23
CGGTCCA	100	3.263267E-9	19.200401	10
CGCAGCT	125	7.2759576E-12	19.19889	31
>>END_MODULE