FastQCFastQC Report
Thu 2 Feb 2017
SRR4062858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271853
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA68740.5404712651540705No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA63210.4969913975907593No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG61040.4799296774076879No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC50300.3954859563172788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35830.2817149466172585No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA34930.2746386571404085No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA32380.25458917028933375No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA27280.2144901965871842No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA26200.20599864921496433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25460.20018036675622103No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT23620.18571328604799453No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA21130.16613555182870976No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA20500.1611821491949148No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT17120.1346067509374118No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG16930.13311286760341015No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG16000.12580070181066522No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT15160.1191961649656053No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC14790.11628702373623367No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA14630.11502901671812701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14510.11408551145454703No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG14260.11211987548875538No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA13640.1072450982935921No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT13160.10347107723927215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGG1101.8189894E-1221.81772631
ACCGGGA453.5366145E-421.3337278
ACCCACG756.4052074E-821.33288814
GGCGGGT1151.8189894E-1220.8691332
CCCGGCG1102.3646862E-1120.3632129
GACCGGG400.004482239520.0011567
CGGAAGC1300.019.69189620
TTGCGAG902.039269E-819.55514718
CTGCCCG1154.5474735E-1119.47785426
CGCCACT507.197525E-419.19959817
TAGGACC16450.019.064934
CGCTTCG852.3970279E-718.82313732
TCCGACT1208.185452E-1118.66627722
ATTGCGA953.7913196E-818.52592817
TTCGTTC1159.422365E-1018.08657829
TGCCCGG1251.4733814E-1017.91962627
ACGCTTC904.368594E-717.77740731
ATAGCGT1006.811388E-817.6010176
CGTATGT1101.063745E-817.45418223
TCCCCGA1201.6607373E-917.33297222
Produced by FastQC (version 0.11.5)