##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1271853
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27562619264962	32.0	32.0	32.0	32.0	32.0
2	31.401032981012744	32.0	32.0	32.0	32.0	32.0
3	31.455800316546014	32.0	32.0	32.0	32.0	32.0
4	31.5373954379948	32.0	32.0	32.0	32.0	32.0
5	31.509297064991003	32.0	32.0	32.0	32.0	32.0
6	35.038133337736355	36.0	36.0	36.0	36.0	36.0
7	35.06066974721135	36.0	36.0	36.0	36.0	36.0
8	34.98343519258908	36.0	36.0	36.0	36.0	36.0
9	35.101863187019255	36.0	36.0	36.0	36.0	36.0
10	34.99113891306621	36.0	36.0	36.0	36.0	36.0
11	35.11637665673627	36.0	36.0	36.0	36.0	36.0
12	35.06122091153616	36.0	36.0	36.0	36.0	36.0
13	35.077540407578546	36.0	36.0	36.0	36.0	36.0
14	35.049526163794084	36.0	36.0	36.0	36.0	36.0
15	35.02111171652699	36.0	36.0	36.0	36.0	36.0
16	35.05006474804871	36.0	36.0	36.0	36.0	36.0
17	35.06356788087932	36.0	36.0	36.0	36.0	36.0
18	35.06110140086944	36.0	36.0	36.0	36.0	36.0
19	35.00909774950407	36.0	36.0	36.0	36.0	36.0
20	34.99926799716634	36.0	36.0	36.0	36.0	36.0
21	34.9679082409681	36.0	36.0	36.0	36.0	36.0
22	34.955507436787116	36.0	36.0	36.0	36.0	36.0
23	34.926647183282974	36.0	36.0	36.0	36.0	36.0
24	34.91161321316221	36.0	36.0	36.0	36.0	36.0
25	34.88724954849342	36.0	36.0	36.0	36.0	36.0
26	34.84132914731498	36.0	36.0	36.0	32.0	36.0
27	34.77084537285362	36.0	36.0	36.0	32.0	36.0
28	34.72445557780655	36.0	36.0	36.0	32.0	36.0
29	34.70075944311174	36.0	36.0	36.0	32.0	36.0
30	34.639970971488054	36.0	36.0	36.0	32.0	36.0
31	34.63125927288767	36.0	36.0	36.0	32.0	36.0
32	34.6102324718344	36.0	36.0	36.0	32.0	36.0
33	34.631247479071874	36.0	36.0	36.0	32.0	36.0
34	34.5909197053433	36.0	36.0	36.0	32.0	36.0
35	34.582908559401126	36.0	36.0	36.0	32.0	36.0
36	34.5162900115029	36.0	36.0	36.0	32.0	36.0
37	34.44639278281374	36.0	36.0	36.0	32.0	36.0
38	34.099192280868934	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	13.0
22	59.0
23	173.0
24	515.0
25	1420.0
26	3201.0
27	6621.0
28	12180.0
29	20442.0
30	32255.0
31	46838.0
32	67025.0
33	107310.0
34	272629.0
35	701164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.262961189363544	22.599684369198396	13.740240725494775	25.397113715943288
2	10.704932095139926	22.367364781936278	47.73822131960219	19.18948180332161
3	17.0838925567656	28.890838799766954	31.496800337774882	22.528468305692563
4	9.964075924107343	19.150072060441136	41.42229148271532	29.4635605327362
5	9.442608102200571	42.07950296103635	36.745470384919	11.732418551844082
6	27.813928035651948	40.794133279658325	19.858599899988363	11.533338784701366
7	25.168317937113606	33.31433212590488	24.402267879284413	17.1150820576971
8	21.69443922444939	43.99478227640883	20.861144549656828	13.449633949484952
9	26.331229743181677	15.36042786803523	22.30235399758137	36.00598839120172
10	14.754688705199504	30.564075629426192	35.66671095969361	19.0145247056807
11	32.016232009863124	21.43192997685185	29.965372131642514	16.58646588164251
12	25.09879340737794	24.744190727493738	34.87820066265884	15.278815202469481
13	29.542532891038338	24.384302878245958	27.006937133359177	19.06622709735653
14	18.088961538794184	26.609364446991908	28.45030046711373	26.851373547100177
15	18.718043673286143	40.72396731383265	22.977262309402107	17.580726703479097
16	17.16309536258571	29.306892080911993	34.930035043413106	18.59997751308919
17	17.539762849951998	33.529110675526184	31.158003322711036	17.773123151810783
18	18.232531589735608	27.769011041370344	38.09339601353301	15.905061355361036
19	23.419687652582493	26.533569524150984	29.117751815657943	20.928991007608584
20	23.902683722096814	29.481866221961184	30.59614593824915	16.019304117692844
21	20.650689428242774	27.66794223537191	27.841704727990933	23.83966360839438
22	22.543957517103	33.39513292809782	28.190050265242917	15.870859289556261
23	17.288188632158956	33.20703447180527	31.434185293717583	18.070591602318196
24	22.533107206571827	28.28322141002144	32.298150808308826	16.88552057509791
25	19.623730100884302	30.90797442786234	31.960140047631292	17.508155423622068
26	15.992033664294272	32.91829552495102	33.05872066875706	18.030950141997653
27	18.14697982777816	32.16744453739755	31.028236078525108	18.657339556299178
28	16.885284698782012	32.84239609451721	29.217212995527	21.055106211173776
29	19.88712532030038	29.827425024747356	28.517603842582435	21.767845812369828
30	17.186577379618555	30.162605269634145	31.45945325442484	21.191364096322452
31	21.811718807126297	27.99765381691123	30.516498368915272	19.6741290070472
32	18.48468337142736	31.948660733591066	30.71078182777412	18.855874067207452
33	16.537131256520997	32.688447485676406	33.652159486984736	17.122261770817854
34	18.918931668990048	31.65939774486517	32.825177123456875	16.596493462687906
35	17.701731253533232	32.853089154171116	31.92035557568367	17.524824016611984
36	16.575972223205042	31.27845749469475	33.65837089663664	18.48719938546357
37	17.49274483765026	31.332551796473336	28.951380387513336	22.223322978363065
38	18.797548771397928	34.14941361101763	28.87725930375547	18.175778313828967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	169.5
2	117.0
3	117.0
4	402.5
5	688.0
6	688.0
7	862.0
8	1036.0
9	1067.5
10	1099.0
11	1099.0
12	1465.5
13	1832.0
14	2605.0
15	3378.0
16	3378.0
17	5472.5
18	7567.0
19	7567.0
20	9985.5
21	12404.0
22	16120.0
23	19836.0
24	19836.0
25	28734.5
26	37633.0
27	37633.0
28	51852.0
29	66071.0
30	91303.0
31	116535.0
32	116535.0
33	144115.0
34	171695.0
35	171695.0
36	178483.5
37	185272.0
38	184071.0
39	182870.0
40	182870.0
41	158839.5
42	134809.0
43	113263.0
44	91717.0
45	91717.0
46	79182.0
47	66647.0
48	66647.0
49	58097.5
50	49548.0
51	42165.5
52	34783.0
53	34783.0
54	29267.5
55	23752.0
56	23752.0
57	21052.0
58	18352.0
59	15197.0
60	12042.0
61	12042.0
62	11824.5
63	11607.0
64	9268.0
65	6929.0
66	6929.0
67	5903.5
68	4878.0
69	4878.0
70	4041.0
71	3204.0
72	2480.0
73	1756.0
74	1756.0
75	1318.5
76	881.0
77	881.0
78	1003.5
79	1126.0
80	840.0
81	554.0
82	554.0
83	615.5
84	677.0
85	677.0
86	422.0
87	167.0
88	150.0
89	133.0
90	133.0
91	77.0
92	21.0
93	15.5
94	10.0
95	10.0
96	6.5
97	3.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008963300004009897
2	0.0
3	0.0
4	3.1450175452666306E-4
5	7.862543863166577E-5
6	7.862543863166577E-5
7	3.1450175452666306E-4
8	0.002673264913476636
9	0.00463890087926828
10	0.0015725087726333152
11	0.003538144738424959
12	5.503780704216603E-4
13	1.5725087726333153E-4
14	0.0
15	0.0
16	1.5725087726333153E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5725087726333153E-4
22	0.0
23	1.5725087726333153E-4
24	0.0
25	0.0
26	7.862543863166577E-5
27	3.931271931583288E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.862543863166577E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1271853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.783473628262726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.60411507892648	50.32107241212842
2	9.394497822740693	11.044824300276982
3	2.023257422145721	3.5680229795366945
4	0.7950558233680546	1.8694457210381095
5	0.4421873946385753	1.299665552574345
6	0.2928952008260528	1.0330438388161793
7	0.20609951059917692	0.8480671602273201
8	0.15403533703842648	0.7243785738095124
9	0.1247446126962857	0.6599629485629963
>10	0.7933701920211884	9.073288316524703
>50	0.09346865520573128	3.7954920852566048
>100	0.06710942354165148	7.797402064718581
>500	0.004716520864998093	1.9523397900685573
>1k	0.00390797443099842	4.0857657101418505
>5k	5.390309559997821E-4	1.9272285463189942
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	6874	0.5404712651540705	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	6321	0.4969913975907593	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	6104	0.4799296774076879	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	5030	0.3954859563172788	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3583	0.2817149466172585	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	3493	0.2746386571404085	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	3238	0.25458917028933375	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2728	0.2144901965871842	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	2620	0.20599864921496433	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2546	0.20018036675622103	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	2362	0.18571328604799453	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2113	0.16613555182870976	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	2050	0.1611821491949148	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	1712	0.1346067509374118	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	1693	0.13311286760341015	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	1600	0.12580070181066522	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	1516	0.1191961649656053	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	1479	0.11628702373623367	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	1463	0.11502901671812701	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1451	0.11408551145454703	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	1426	0.11211987548875538	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	1364	0.1072450982935921	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	1316	0.10347107723927215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.862543863166577E-5	0.0
6	0.0	0.0	0.0	7.862543863166577E-5	0.0
7	0.0	0.0	0.0	7.862543863166577E-5	0.0
8	0.0	0.0	0.0	7.862543863166577E-5	0.0
9	0.0	0.0	0.0	7.862543863166577E-5	0.0
10	0.0	0.0	0.0	7.862543863166577E-5	0.0
11	0.0	0.0	0.0	7.862543863166577E-5	0.0
12	0.0	0.0	0.0	7.862543863166577E-5	0.0
13	0.0	0.0	0.0	7.862543863166577E-5	0.0
14	0.0	0.0	0.0	7.862543863166577E-5	0.0
15	0.0	0.0	0.0	7.862543863166577E-5	0.0
16	0.0	0.0	0.0	7.862543863166577E-5	0.0
17	0.0	0.0	0.0	7.862543863166577E-5	0.0
18	0.0	0.0	0.0	7.862543863166577E-5	0.0
19	0.0	0.0	0.0	7.862543863166577E-5	0.0
20	0.0	0.0	0.0	2.358763158949973E-4	0.0
21	0.0	0.0	0.0	3.9312719315832886E-4	0.0
22	0.0	0.0	0.0	5.503780704216604E-4	0.0
23	0.0	0.0	0.0	9.435052635799892E-4	0.0
24	0.0	0.0	0.0	0.001651134211264981	0.0
25	0.0	0.0	0.0	0.0024373885975816387	0.0
26	0.0	0.0	0.0	0.002987766668003299	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGGG	110	1.8189894E-12	21.817726	31
ACCGGGA	45	3.5366145E-4	21.333727	8
ACCCACG	75	6.4052074E-8	21.332888	14
GGCGGGT	115	1.8189894E-12	20.86913	32
CCCGGCG	110	2.3646862E-11	20.36321	29
GACCGGG	40	0.0044822395	20.001156	7
CGGAAGC	130	0.0	19.691896	20
TTGCGAG	90	2.039269E-8	19.555147	18
CTGCCCG	115	4.5474735E-11	19.477854	26
CGCCACT	50	7.197525E-4	19.199598	17
TAGGACC	1645	0.0	19.06493	4
CGCTTCG	85	2.3970279E-7	18.823137	32
TCCGACT	120	8.185452E-11	18.666277	22
ATTGCGA	95	3.7913196E-8	18.525928	17
TTCGTTC	115	9.422365E-10	18.086578	29
TGCCCGG	125	1.4733814E-10	17.919626	27
ACGCTTC	90	4.368594E-7	17.777407	31
ATAGCGT	100	6.811388E-8	17.601017	6
CGTATGT	110	1.063745E-8	17.454182	23
TCCCCGA	120	1.6607373E-9	17.332972	22
>>END_MODULE