FastQCFastQC Report
Thu 2 Feb 2017
SRR4062857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680967
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39240.23343706330939276No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26770.15925357249725902No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26360.15681450022516802No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25890.15401849054740516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25700.1528881887627776No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24000.14277496226874173No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22410.1333161210184376No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22390.13319714188321363No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22250.132364287936646No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21780.12956827825888312No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21690.12903287215037534No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20510.12201310317216223No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20320.12088280138753467No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19270.11463639678827722No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18860.11219732451618622No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18550.11035314792021497No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18420.1095797835412593No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18080.10755713824245211No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17350.10321439980677789No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17260.1026789936982701No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17160.10208409802215035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG350.002070748222.8498975
CGTACAC601.140831E-418.661863
ATCGTTT4850.018.14397629
GTCGTAT450.0088135417.7911951
TCGTTTA5050.017.74170130
GGCATCG5350.017.64451626
GCATCGT5200.017.53811827
CGTTTAT5150.017.39720231
CCACGCG652.1009662E-417.23094624
CGGCATC5200.017.23043325
GTCGTAA854.9890314E-616.94034230
GCGCAAG6700.016.4900461
CGTCGTA5050.016.47688710
CGCAAGA6650.016.362532
CATCGTT5400.016.29597928
ACCGTCG5150.016.1540628
GTCCTAC5500.016.0120751
TCTAGCG5400.015.99968828
ATCGCCA5800.015.72570316
TACCGTC5500.015.707857
Produced by FastQC (version 0.11.5)