##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062857_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1680967
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218141700580677	32.0	32.0	32.0	32.0	32.0
2	30.7834282291086	32.0	32.0	32.0	32.0	32.0
3	30.837823705045963	32.0	32.0	32.0	32.0	32.0
4	30.913433755689432	32.0	32.0	32.0	32.0	32.0
5	30.786788199887326	32.0	32.0	32.0	32.0	32.0
6	34.44918430879369	36.0	36.0	36.0	32.0	36.0
7	34.37843217624141	36.0	36.0	36.0	32.0	36.0
8	34.35601650716522	36.0	36.0	36.0	32.0	36.0
9	34.52802166848011	36.0	36.0	36.0	32.0	36.0
10	34.2005161314886	36.0	36.0	36.0	32.0	36.0
11	34.5105811119433	36.0	36.0	36.0	32.0	36.0
12	34.29938184390294	36.0	36.0	36.0	32.0	36.0
13	34.40909904834539	36.0	36.0	36.0	32.0	36.0
14	34.28333036876988	36.0	36.0	36.0	32.0	36.0
15	34.21246580093482	36.0	36.0	36.0	32.0	36.0
16	34.24976694961888	36.0	36.0	36.0	32.0	36.0
17	34.16357073041886	36.0	36.0	36.0	32.0	36.0
18	34.187936467521375	36.0	36.0	36.0	32.0	36.0
19	34.16656008119136	36.0	36.0	36.0	32.0	36.0
20	34.1631239637661	36.0	36.0	36.0	32.0	36.0
21	34.14619025834534	36.0	36.0	36.0	32.0	36.0
22	34.11071900876103	36.0	36.0	36.0	32.0	36.0
23	34.0535525087643	36.0	36.0	36.0	32.0	36.0
24	34.039382688654804	36.0	36.0	36.0	32.0	36.0
25	34.01889626625627	36.0	36.0	36.0	32.0	36.0
26	33.957093149359864	36.0	36.0	36.0	32.0	36.0
27	33.967820308191655	36.0	36.0	36.0	32.0	36.0
28	33.94731663381851	36.0	36.0	36.0	32.0	36.0
29	33.91060026758407	36.0	36.0	36.0	32.0	36.0
30	33.886280337448625	36.0	36.0	36.0	32.0	36.0
31	33.90907852444456	36.0	36.0	36.0	32.0	36.0
32	33.854706249438564	36.0	36.0	36.0	32.0	36.0
33	33.8216401630728	36.0	36.0	36.0	32.0	36.0
34	33.82639813869041	36.0	36.0	36.0	32.0	36.0
35	33.78448238424669	36.0	36.0	36.0	32.0	36.0
36	33.73921974672911	36.0	36.0	36.0	32.0	36.0
37	33.73984081781499	36.0	36.0	36.0	32.0	36.0
38	33.1285539811311	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	5.0
10	15.0
11	19.0
12	6.0
13	7.0
14	156.0
15	428.0
16	515.0
17	633.0
18	809.0
19	1013.0
20	1407.0
21	2110.0
22	3412.0
23	5130.0
24	7671.0
25	11651.0
26	17262.0
27	25104.0
28	35041.0
29	47388.0
30	63355.0
31	85940.0
32	116767.0
33	174556.0
34	377622.0
35	702941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.754410205710336	17.108858527985895	11.315497957190333	25.821233309113435
2	16.859322817745753	19.309520989411997	37.45655787809005	26.374598314752195
3	19.057999684671152	23.46183479743096	28.16904006163787	29.311125456260022
4	12.438553250676561	14.941636370883446	35.94748611926633	36.672324259173664
5	14.789974556251387	36.4054414047186	33.324647835794124	15.479936203235884
6	34.80917474636547	35.42025340251594	16.56438396584813	13.20618788527046
7	30.790491425471174	30.127123257030032	20.535620270951185	18.546765046547613
8	28.59043977146447	32.671609861960164	19.102372210231746	19.635578156343623
9	27.64083271166698	13.876247348250947	18.36741492689323	40.115505013188844
10	16.09274927095318	26.21677349041799	30.94513736776917	26.745339870859663
11	38.22410291372338	20.823086102911343	21.883205964274794	19.069605019090485
12	25.17002553357558	23.3732161596836	28.413420237916764	23.043338068824053
13	29.70621029245695	18.92955782203643	25.150024453718217	26.214207431788406
14	23.815680112707092	19.720945230801448	24.590600880405184	31.872773776086273
15	25.30111592293622	27.21104950546894	22.07098493260073	25.41684963899411
16	26.126780046228397	25.390754915114066	23.763241580570984	24.719223458086546
17	24.220845097240034	25.76954543967013	24.860662785197263	25.14894667789258
18	25.4540338168706	24.273757092279805	26.194064170073123	24.078144920776477
19	25.915210403351175	24.654057517567075	25.105557151976715	24.32517492710503
20	26.125249275376117	23.953102913249786	24.614961757944688	25.306686053429413
21	27.41524022112385	23.881589750057707	24.003550546503355	24.69961948231509
22	26.193310642419398	24.09960680584633	24.67282902866349	25.03425352307078
23	24.41465861183926	23.847314455971926	25.52638186021773	26.21164507197108
24	25.156999204517533	24.740694729289046	25.131296255639622	24.9710098105538
25	25.055854537232886	24.320805852306027	25.130406585331748	25.492933025129332
26	24.62201378844196	25.529501994129767	25.590905602067732	24.257578615360543
27	25.65580773285547	24.39803477809746	24.715556856501678	25.230600632545386
28	24.53064791618205	24.522913876711083	25.6493469793519	25.29709122775497
29	24.566427217483085	24.708621241526366	25.480931126926315	25.244020414064238
30	24.377155816048134	25.059818503936537	25.762113321411277	24.800912358604048
31	25.43996325731953	24.844798465564743	24.29776325363099	25.417475023484737
32	24.916307275302806	24.70855536867932	24.35706674773671	26.018070608281164
33	24.367681101736814	24.382197678894503	25.19756526064098	26.05255595872771
34	25.534204878838196	24.63187981215741	25.24404007927324	24.589875229731152
35	26.064887783011653	24.293679423722136	25.392139188215506	24.2492936050507
36	24.382501930704084	25.29258050351811	24.900608900635078	25.424308665142732
37	25.735255183360888	24.997397089857216	24.52209082401293	24.74525690276896
38	24.713793536311915	24.740089489531293	25.159813358225247	25.38630361593154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	122.5
2	177.0
3	177.0
4	449.0
5	721.0
6	721.0
7	901.0
8	1081.0
9	1092.5
10	1104.0
11	1104.0
12	1465.5
13	1827.0
14	2464.5
15	3102.0
16	3102.0
17	4710.0
18	6318.0
19	6318.0
20	7755.5
21	9193.0
22	9957.0
23	10721.0
24	10721.0
25	13380.0
26	16039.0
27	16039.0
28	20591.0
29	25143.0
30	31221.0
31	37299.0
32	37299.0
33	47972.0
34	58645.0
35	58645.0
36	65748.5
37	72852.0
38	82502.5
39	92153.0
40	92153.0
41	97116.5
42	102080.0
43	115549.5
44	129019.0
45	129019.0
46	131108.5
47	133198.0
48	133198.0
49	142220.0
50	151242.0
51	154909.5
52	158577.0
53	158577.0
54	151478.5
55	144380.0
56	144380.0
57	140856.0
58	137332.0
59	122831.0
60	108330.0
61	108330.0
62	103799.5
63	99269.0
64	82373.0
65	65477.0
66	65477.0
67	54896.5
68	44316.0
69	44316.0
70	35812.0
71	27308.0
72	21257.5
73	15207.0
74	15207.0
75	11400.0
76	7593.0
77	7593.0
78	7551.0
79	7509.0
80	5972.0
81	4435.0
82	4435.0
83	4470.0
84	4505.0
85	4505.0
86	3055.5
87	1606.0
88	1325.5
89	1045.0
90	1045.0
91	788.5
92	532.0
93	467.0
94	402.0
95	402.0
96	360.5
97	319.0
98	319.0
99	581.0
100	843.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11344660543603771
2	0.051042049011075175
3	0.010827101305379581
4	0.002379582704479029
5	2.379582704479029E-4
6	2.974478380598786E-4
7	0.0
8	1.7846870283592717E-4
9	5.9489567611975724E-5
10	2.974478380598786E-4
11	0.001487239190299393
12	0.002558051407314956
13	0.014336985794486149
14	0.008923435141796358
15	0.022903483530610655
16	0.010827101305379581
17	0.01975053644717594
18	0.005354061085077816
19	0.00737670638388499
20	0.005651508923137693
21	0.005889467193585597
22	0.007019768978213136
23	0.009101903844632286
24	0.013444642280306514
25	0.015586266714337639
26	0.016895037201801105
27	0.008269049898064625
28	0.005116102814629912
29	0.009815778655975995
30	0.0029149888129868106
31	0.005235081949853864
32	0.006900789842989185
33	0.0077336437895568445
34	0.011778934387171194
35	0.014872391902993934
36	0.013861069253590343
37	0.009280372547468213
38	0.005948956761197572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1680967.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.36158158653251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.04630579117395	39.57220120834313
2	13.619324569871708	13.990201000978649
3	4.37732158818315	6.7447847964587675
4	1.8570248807633682	3.8151893968619413
5	0.9104857836125977	2.3381994929198213
6	0.530407180536967	1.6345531006339262
7	0.33206155414187477	1.193864462336608
8	0.21971160885886984	0.902778857913053
9	0.16065772654058222	0.7426471436298848
>10	0.7777425406223172	7.044586521840614
>50	0.06865908288174256	2.484687154685643
>100	0.07991225679798612	9.12812824466636
>500	0.013745608284403259	4.88417731091586
>1k	0.006639827730601575	5.524001307815856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3924	0.23343706330939276	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2677	0.15925357249725902	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2636	0.15681450022516802	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2589	0.15401849054740516	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2570	0.1528881887627776	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2400	0.14277496226874173	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2241	0.1333161210184376	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2239	0.13319714188321363	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2225	0.132364287936646	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2178	0.12956827825888312	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2169	0.12903287215037534	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2051	0.12201310317216223	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2032	0.12088280138753467	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1927	0.11463639678827722	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1886	0.11219732451618622	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1855	0.11035314792021497	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1842	0.1095797835412593	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1808	0.10755713824245211	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1735	0.10321439980677789	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1726	0.1026789936982701	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1716	0.10208409802215035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.7846870283592717E-4
7	0.0	0.0	0.0	0.0	1.7846870283592717E-4
8	0.0	0.0	0.0	0.0	1.7846870283592717E-4
9	0.0	0.0	0.0	0.0	1.7846870283592717E-4
10	0.0	0.0	0.0	5.9489567611975724E-5	1.7846870283592717E-4
11	0.0	0.0	0.0	1.1897913522395145E-4	1.7846870283592717E-4
12	0.0	0.0	0.0	1.7846870283592717E-4	1.7846870283592717E-4
13	0.0	0.0	0.0	1.7846870283592717E-4	1.7846870283592717E-4
14	0.0	0.0	0.0	1.7846870283592717E-4	1.7846870283592717E-4
15	0.0	0.0	0.0	1.7846870283592717E-4	1.7846870283592717E-4
16	0.0	0.0	0.0	2.379582704479029E-4	2.379582704479029E-4
17	0.0	0.0	0.0	2.379582704479029E-4	2.379582704479029E-4
18	0.0	0.0	0.0	3.5693740567185433E-4	2.379582704479029E-4
19	0.0	0.0	0.0	4.1642697328383006E-4	2.379582704479029E-4
20	0.0	0.0	0.0	4.1642697328383006E-4	3.5693740567185433E-4
21	0.0	0.0	0.0	5.354061085077815E-4	3.5693740567185433E-4
22	0.0	0.0	0.0	6.54385243731733E-4	4.1642697328383006E-4
23	0.0	0.0	0.0	0.001070812217015563	4.759165408958058E-4
24	0.0	0.0	0.0	0.0017846870283592718	4.759165408958058E-4
25	0.0	0.0	0.0	0.002141624434031126	4.759165408958058E-4
26	0.0	0.0	0.0	0.002677030542538908	5.354061085077815E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCG	35	0.0020707482	22.849897	5
CGTACAC	60	1.140831E-4	18.66186	3
ATCGTTT	485	0.0	18.143976	29
GTCGTAT	45	0.00881354	17.791195	1
TCGTTTA	505	0.0	17.741701	30
GGCATCG	535	0.0	17.644516	26
GCATCGT	520	0.0	17.538118	27
CGTTTAT	515	0.0	17.397202	31
CCACGCG	65	2.1009662E-4	17.230946	24
CGGCATC	520	0.0	17.230433	25
GTCGTAA	85	4.9890314E-6	16.940342	30
GCGCAAG	670	0.0	16.490046	1
CGTCGTA	505	0.0	16.476887	10
CGCAAGA	665	0.0	16.36253	2
CATCGTT	540	0.0	16.295979	28
ACCGTCG	515	0.0	16.154062	8
GTCCTAC	550	0.0	16.012075	1
TCTAGCG	540	0.0	15.999688	28
ATCGCCA	580	0.0	15.725703	16
TACCGTC	550	0.0	15.70785	7
>>END_MODULE