FastQCFastQC Report
Thu 2 Feb 2017
SRR4062857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680967
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41570.24729813256298308No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC30030.1786471715387631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28370.16877190331517514No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26680.15871816638875125No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25360.15086554346397044No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24530.14592790935217645No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24490.14568995108172855No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23830.14176363961933816No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21810.12974674696171906No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21470.12772410166291187No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21080.12540400852604483No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20850.12403574847096939No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20650.12284595711872988No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20560.12231055101022208No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20150.11987147873813107No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19370.11523129246439698No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17810.10595091991692877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17740.10553449294364495No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG17480.10398776418573358No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG17180.1022030771573743No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17060.10148920234603058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4950.019.71662129
CTTACGC601.1380445E-418.6678123
ATAGTAC1055.979018E-918.2868373
TCTAGCG5300.018.11270328
TAGCGGC5550.017.87337530
CATCGTT5400.016.88841828
GTAGGAC5150.016.467033
ATCGTTT5550.016.43197429
TATACTG2100.016.0005055
TGTAGGA5400.016.0000292
ATCCGCC600.002442991415.99955431
GTCCTAT6250.015.8743911
AGCGGCG6050.015.86732731
GCATCGT5850.015.5893127
ACCGTCG5250.015.5433488
ATACCGT5100.015.3730356
GGCATCG5950.015.32730426
CCTATTC5750.015.3052873
AAGACGG6400.015.2504825
TCGTTTA6000.015.19957630
Produced by FastQC (version 0.11.5)