##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062857_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1680967
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16493542109988	32.0	32.0	32.0	32.0	32.0
2	31.217430205352038	32.0	32.0	32.0	32.0	32.0
3	31.318241226627293	32.0	32.0	32.0	32.0	32.0
4	31.433258951543962	32.0	32.0	32.0	32.0	32.0
5	31.33881926295995	32.0	32.0	32.0	32.0	32.0
6	34.855599187848426	36.0	36.0	36.0	32.0	36.0
7	34.88358129576607	36.0	36.0	36.0	32.0	36.0
8	34.81476554863956	36.0	36.0	36.0	32.0	36.0
9	34.93854608686548	36.0	36.0	36.0	32.0	36.0
10	34.772744497661165	36.0	36.0	36.0	32.0	36.0
11	34.96167325117031	36.0	36.0	36.0	36.0	36.0
12	34.85336178521054	36.0	36.0	36.0	32.0	36.0
13	34.914069104271526	36.0	36.0	36.0	32.0	36.0
14	34.84971447982025	36.0	36.0	36.0	32.0	36.0
15	34.821108326338354	36.0	36.0	36.0	32.0	36.0
16	34.83211865551198	36.0	36.0	36.0	32.0	36.0
17	34.795240477653635	36.0	36.0	36.0	32.0	36.0
18	34.79775629146795	36.0	36.0	36.0	32.0	36.0
19	34.78966511537704	36.0	36.0	36.0	32.0	36.0
20	34.7752073657603	36.0	36.0	36.0	32.0	36.0
21	34.77238815515117	36.0	36.0	36.0	32.0	36.0
22	34.75790244543766	36.0	36.0	36.0	32.0	36.0
23	34.68904386582247	36.0	36.0	36.0	32.0	36.0
24	34.665949420779825	36.0	36.0	36.0	32.0	36.0
25	34.64637021428737	36.0	36.0	36.0	32.0	36.0
26	34.5728155282049	36.0	36.0	36.0	32.0	36.0
27	34.56105206110531	36.0	36.0	36.0	32.0	36.0
28	34.528707583194674	36.0	36.0	36.0	32.0	36.0
29	34.48622727275431	36.0	36.0	36.0	32.0	36.0
30	34.45869907023755	36.0	36.0	36.0	32.0	36.0
31	34.467705790774	36.0	36.0	36.0	32.0	36.0
32	34.4372911544367	36.0	36.0	36.0	32.0	36.0
33	34.40008756864353	36.0	36.0	36.0	32.0	36.0
34	34.40118872054002	36.0	36.0	36.0	32.0	36.0
35	34.35512356875537	36.0	36.0	36.0	32.0	36.0
36	34.32798323821943	36.0	36.0	36.0	32.0	36.0
37	34.31803658251471	36.0	36.0	36.0	32.0	36.0
38	33.86928357308621	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	32.0
22	125.0
23	390.0
24	1229.0
25	2933.0
26	6239.0
27	12010.0
28	20904.0
29	32684.0
30	49209.0
31	71234.0
32	103111.0
33	165953.0
34	397140.0
35	817766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.983227473816726	18.331623874672488	12.035025261118818	26.650123390391972
2	15.761285022252073	20.393261735655727	36.723683451251574	27.121769790840627
3	17.94092328998725	24.30773477409134	28.519893608857284	29.231448327064125
4	11.93531557246123	15.862592885855175	35.99048401479868	36.21160752688492
5	14.293158989033087	36.90624135541192	33.02168694767589	15.778912707879106
6	34.83558819295142	35.805508988305505	16.141292902516536	13.217609916226541
7	30.528970281338562	30.343957395875226	20.534288658174717	18.5927836646115
8	28.392046793088593	32.96956688313388	18.93818429299936	19.700202030778165
9	27.526651043999877	13.761927453951506	18.129016099199767	40.58240540284885
10	15.798484900764212	26.390913211965167	31.02354269560117	26.787059191669453
11	38.33997268110665	20.745939051263377	21.831436716783035	19.082651550846933
12	24.962670274116192	23.48863689093057	28.400158004385574	23.148534830567662
13	29.6308546762453	18.91837064922271	25.188879714259198	26.261894960272798
14	23.54608983995522	19.80711102597493	24.62314846157004	32.02365067249981
15	25.189608124371272	27.33825232738061	21.916908541333648	25.555231006914475
16	26.031876908872636	25.545608893933608	23.51939301568265	24.903121181511107
17	24.27281439790311	25.84405285767061	24.773657067628335	25.10947567679794
18	25.380807594676156	24.317193615341644	26.15464789017274	24.147350899809457
19	25.86463624806436	24.686683319779625	25.0198843879743	24.42879604418171
20	26.009136407793847	23.907132025792297	24.642482571043928	25.441248995369925
21	27.37925692726682	23.927789140291953	24.020711900181205	24.672242032260023
22	26.018535759476542	24.09993771442271	24.813753036198808	25.067773489901946
23	24.354447036875314	23.79042476867413	25.618782578071364	26.236345616379193
24	25.057838732110742	24.783175398446254	25.020241325379978	25.13874454406303
25	24.968381276004393	24.267712731845855	25.156249442284974	25.60765654986478
26	24.66960981387499	25.40377056777438	25.559038339241642	24.36758127910899
27	25.70966592443516	24.297026651921186	24.635760249903775	25.357547173739874
28	24.420229546445587	24.514520511110568	25.71216448627486	25.353085456168976
29	24.541826222644467	24.577638942346873	25.550709799775962	25.329825035232695
30	24.337955474438225	24.9056049285917	25.880519962616756	24.875919634353323
31	25.348385780327632	24.723923789104724	24.366450977324362	25.561239453243285
32	25.002215986393544	24.660745868300804	24.424215347475588	25.91282279783006
33	24.35264939763838	24.255859871133698	25.387886853221985	26.003603878005933
34	25.50282069784832	24.40732031027379	25.353501883142265	24.736357108735625
35	26.042450565656555	24.253123351023547	25.393835809983184	24.310590273336715
36	24.487631226549954	25.14808440617811	24.89549170209766	25.468792665174274
37	25.80080394201671	24.84242700778778	24.59554530219808	24.761223747997434
38	24.720666569103717	24.472119007761016	25.28748350650757	25.519730916627704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	47.0
2	64.0
3	64.0
4	290.0
5	516.0
6	516.0
7	738.0
8	960.0
9	1035.5
10	1111.0
11	1111.0
12	1504.5
13	1898.0
14	2559.5
15	3221.0
16	3221.0
17	4818.5
18	6416.0
19	6416.0
20	7857.0
21	9298.0
22	10256.5
23	11215.0
24	11215.0
25	13628.5
26	16042.0
27	16042.0
28	20670.0
29	25298.0
30	31404.0
31	37510.0
32	37510.0
33	48347.0
34	59184.0
35	59184.0
36	65925.5
37	72667.0
38	82453.0
39	92239.0
40	92239.0
41	97344.5
42	102450.0
43	116010.0
44	129570.0
45	129570.0
46	131390.0
47	133210.0
48	133210.0
49	142980.5
50	152751.0
51	155087.0
52	157423.0
53	157423.0
54	151016.0
55	144609.0
56	144609.0
57	141176.0
58	137743.0
59	122986.5
60	108230.0
61	108230.0
62	103965.0
63	99700.0
64	82573.5
65	65447.0
66	65447.0
67	55154.0
68	44861.0
69	44861.0
70	36125.0
71	27389.0
72	21199.5
73	15010.0
74	15010.0
75	11186.5
76	7363.0
77	7363.0
78	7392.5
79	7422.0
80	5884.5
81	4347.0
82	4347.0
83	4058.5
84	3770.0
85	3770.0
86	2409.0
87	1048.0
88	872.5
89	697.0
90	697.0
91	423.0
92	149.0
93	105.0
94	61.0
95	61.0
96	42.0
97	23.0
98	23.0
99	24.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007317216816273014
2	0.0
3	0.0
4	3.5693740567185433E-4
5	1.1897913522395145E-4
6	2.974478380598786E-4
7	1.7846870283592717E-4
8	0.0020821348664191506
9	0.004521207138510155
10	0.00124928091985149
11	0.004699675841346083
12	5.9489567611975724E-5
13	1.7846870283592717E-4
14	0.0
15	0.0
16	5.9489567611975724E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	5.9489567611975724E-5
22	0.0
23	2.974478380598786E-4
24	0.0
25	5.9489567611975724E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.9489567611975724E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1680967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.9915020530515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9293022417029	36.70899064608104
2	14.789990520565613	14.491677019058047
3	4.829595096302251	7.098273542276974
4	2.0505074893928743	4.018297675055539
5	0.9964853953599309	2.440965814630594
6	0.5873237639836153	1.7264324033405534
7	0.3657497404935089	1.2543040413603557
8	0.2385042620043363	0.934774563331758
9	0.17386718338572676	0.7666213024620103
>10	0.8550662151494797	7.361924848545918
>50	0.07370655244239303	2.5443743659179496
>100	0.08689347762445805	9.362062520946626
>500	0.015420296599615265	5.209220568016741
>1k	0.00758776499346148	6.0820806889760615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4157	0.24729813256298308	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3003	0.1786471715387631	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2837	0.16877190331517514	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2668	0.15871816638875125	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2536	0.15086554346397044	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2453	0.14592790935217645	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2449	0.14568995108172855	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2383	0.14176363961933816	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2181	0.12974674696171906	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2147	0.12772410166291187	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2108	0.12540400852604483	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2085	0.12403574847096939	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2065	0.12284595711872988	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2056	0.12231055101022208	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2015	0.11987147873813107	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1937	0.11523129246439698	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1781	0.10595091991692877	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1774	0.10553449294364495	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1748	0.10398776418573358	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1718	0.1022030771573743	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1706	0.10148920234603058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1897913522395145E-4
2	0.0	0.0	0.0	0.0	1.1897913522395145E-4
3	0.0	0.0	0.0	0.0	1.1897913522395145E-4
4	0.0	0.0	0.0	0.0	1.7846870283592717E-4
5	0.0	0.0	0.0	0.0	1.7846870283592717E-4
6	0.0	0.0	0.0	0.0	2.379582704479029E-4
7	0.0	0.0	0.0	0.0	2.379582704479029E-4
8	0.0	0.0	0.0	0.0	2.379582704479029E-4
9	0.0	0.0	0.0	0.0	2.379582704479029E-4
10	0.0	0.0	0.0	1.1897913522395145E-4	2.379582704479029E-4
11	0.0	0.0	0.0	1.7846870283592717E-4	2.379582704479029E-4
12	0.0	0.0	0.0	1.7846870283592717E-4	4.759165408958058E-4
13	0.0	0.0	0.0	1.7846870283592717E-4	5.354061085077815E-4
14	0.0	0.0	0.0	1.7846870283592717E-4	5.948956761197573E-4
15	0.0	0.0	0.0	1.7846870283592717E-4	7.733643789556844E-4
16	0.0	0.0	0.0	2.379582704479029E-4	7.733643789556844E-4
17	0.0	0.0	0.0	2.379582704479029E-4	7.733643789556844E-4
18	0.0	0.0	0.0	2.379582704479029E-4	7.733643789556844E-4
19	0.0	0.0	0.0	2.9744783805987866E-4	7.733643789556844E-4
20	0.0	0.0	0.0	2.9744783805987866E-4	8.923435141796359E-4
21	0.0	0.0	0.0	4.1642697328383006E-4	9.518330817916116E-4
22	0.0	0.0	0.0	6.54385243731733E-4	9.518330817916116E-4
23	0.0	0.0	0.0	0.001070812217015563	9.518330817916116E-4
24	0.0	0.0	0.0	0.0017846870283592718	0.0010113226494035874
25	0.0	0.0	0.0	0.002141624434031126	0.0010113226494035874
26	0.0	0.0	0.0	0.002558051407314956	0.001070812217015563
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	495	0.0	19.716621	29
CTTACGC	60	1.1380445E-4	18.667812	3
ATAGTAC	105	5.979018E-9	18.286837	3
TCTAGCG	530	0.0	18.112703	28
TAGCGGC	555	0.0	17.873375	30
CATCGTT	540	0.0	16.888418	28
GTAGGAC	515	0.0	16.46703	3
ATCGTTT	555	0.0	16.431974	29
TATACTG	210	0.0	16.000505	5
TGTAGGA	540	0.0	16.000029	2
ATCCGCC	60	0.0024429914	15.999554	31
GTCCTAT	625	0.0	15.874391	1
AGCGGCG	605	0.0	15.867327	31
GCATCGT	585	0.0	15.58931	27
ACCGTCG	525	0.0	15.543348	8
ATACCGT	510	0.0	15.373035	6
GGCATCG	595	0.0	15.327304	26
CCTATTC	575	0.0	15.305287	3
AAGACGG	640	0.0	15.250482	5
TCGTTTA	600	0.0	15.199576	30
>>END_MODULE