FastQCFastQC Report
Thu 2 Feb 2017
SRR4062856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486165
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36340.24452197434336026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24330.16370995145222772No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23880.1606820238667981No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23820.1602783001887408No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21210.14271632019324906No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20920.14076498908263887No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19890.13383439927598886No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19820.13336338831825537No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19790.13316152647922672No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19460.13094104624991168No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19320.1299990243344447No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16920.11385007721215343No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16420.11048571322834273No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15830.10651576372744614No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15690.10557374181197915No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15510.10436257077780732No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15300.10294953790460683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT509.693777E-725.5956386
ATCGTTT4450.018.33642429
CGTCTAT701.7682783E-518.3090881
TATGCCG450.00886014717.7747525
CGTTTAT4700.017.70149631
TCTAGCG5350.017.64363528
AGAACCG3300.017.4515725
CGCGGTC3050.017.31085810
TAGACCG652.1036612E-417.2278355
CATCGTT4800.016.99882128
CTAGCGG5600.016.8565429
TCGTTTA5000.016.63940430
CGGCATC4950.016.16103725
GCATCGT5000.015.99888927
TAGCGGC5950.015.86497930
CAAGACG6050.015.6011734
ACCGTCG3300.015.5140738
AACCGCG3550.015.3223657
ATACCGT3350.015.2809796
CGTCGTA3250.015.26099210
Produced by FastQC (version 0.11.5)