##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062856_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1486165
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243905622861526	32.0	32.0	32.0	32.0	32.0
2	30.82144782039679	32.0	32.0	32.0	32.0	32.0
3	30.869032711711014	32.0	32.0	32.0	32.0	32.0
4	30.949677189275754	32.0	32.0	32.0	32.0	32.0
5	30.838065759858427	32.0	32.0	32.0	32.0	32.0
6	34.49905360441135	36.0	36.0	36.0	32.0	36.0
7	34.439924907395884	36.0	36.0	36.0	32.0	36.0
8	34.40150521644636	36.0	36.0	36.0	32.0	36.0
9	34.574397862956	36.0	36.0	36.0	32.0	36.0
10	34.26176837699717	36.0	36.0	36.0	32.0	36.0
11	34.549817819690276	36.0	36.0	36.0	32.0	36.0
12	34.347918972657816	36.0	36.0	36.0	32.0	36.0
13	34.462245443810076	36.0	36.0	36.0	32.0	36.0
14	34.346821517126294	36.0	36.0	36.0	32.0	36.0
15	34.28141760840822	36.0	36.0	36.0	32.0	36.0
16	34.299252774759196	36.0	36.0	36.0	32.0	36.0
17	34.22323698916338	36.0	36.0	36.0	32.0	36.0
18	34.25431429215464	36.0	36.0	36.0	32.0	36.0
19	34.23954069702893	36.0	36.0	36.0	32.0	36.0
20	34.22583831539566	36.0	36.0	36.0	32.0	36.0
21	34.207830893608715	36.0	36.0	36.0	32.0	36.0
22	34.183970151362736	36.0	36.0	36.0	32.0	36.0
23	34.128392876968576	36.0	36.0	36.0	32.0	36.0
24	34.1144637372028	36.0	36.0	36.0	32.0	36.0
25	34.0889235044561	36.0	36.0	36.0	32.0	36.0
26	34.04525473281903	36.0	36.0	36.0	32.0	36.0
27	34.050104127065296	36.0	36.0	36.0	32.0	36.0
28	34.040771381374206	36.0	36.0	36.0	32.0	36.0
29	34.000588763697166	36.0	36.0	36.0	32.0	36.0
30	33.97446447736288	36.0	36.0	36.0	32.0	36.0
31	33.999448244306656	36.0	36.0	36.0	32.0	36.0
32	33.943338727530254	36.0	36.0	36.0	32.0	36.0
33	33.91194248283333	36.0	36.0	36.0	32.0	36.0
34	33.915366732496054	36.0	36.0	36.0	32.0	36.0
35	33.87869112783574	36.0	36.0	36.0	32.0	36.0
36	33.8234624015503	36.0	36.0	36.0	32.0	36.0
37	33.8412316263672	36.0	36.0	36.0	32.0	36.0
38	33.24569681024651	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	6.0
9	11.0
10	12.0
11	18.0
12	10.0
13	11.0
14	135.0
15	332.0
16	456.0
17	510.0
18	643.0
19	844.0
20	1162.0
21	1745.0
22	2690.0
23	4279.0
24	6471.0
25	10008.0
26	14638.0
27	21335.0
28	29803.0
29	40049.0
30	53591.0
31	72839.0
32	99230.0
33	149988.0
34	328655.0
35	646691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77866345451655	17.081659215664082	11.2230558003293	25.91662152949007
2	17.20896601590745	19.333998500008754	37.06924061933211	26.387794864751683
3	18.92007930024132	23.462817581181135	28.34626964504663	29.27083347353092
4	12.632315585835647	14.82573807721423	35.843485574901166	36.69846076204895
5	14.997883812206334	36.21372621980227	33.22145686576775	15.566933102223645
6	34.77286077734617	35.39580892757774	16.542240769661927	13.289089525414171
7	30.760266027167933	30.21678630353043	20.57451263790537	18.448435031396265
8	28.610993731479063	32.37452864975144	19.184728924823506	19.82974869394599
9	27.643273700862427	13.920515907113574	18.6785532369013	39.7576571551227
10	16.24581554413907	26.024270685090045	30.844427398218894	26.885486372551988
11	38.08643283707629	21.037126755824712	21.877634788459922	18.99880561863908
12	25.156465432259097	23.504300822751464	28.150387151430817	23.188846593558623
13	29.52145021286676	18.933162663048673	25.28477575297495	26.26061137110961
14	23.940150297209467	19.68581194073442	24.59574061449984	31.778297147556273
15	25.44494153485395	26.978301296914637	22.03078969171944	25.545967476511972
16	26.322264133166883	25.413959372928517	23.278497666483844	24.98527882742075
17	24.346900220016302	25.614433715462187	24.927261359039814	25.1114047054817
18	25.56636721510043	24.248241983782513	25.862521449480163	24.32286935163689
19	26.01672520105705	24.527378819007268	25.198163417609848	24.257732562325835
20	26.137355431184673	23.83582863914372	24.61426445957322	25.41255147009839
21	27.540596175470494	23.682352885758718	24.089811909377882	24.687239029392902
22	26.20982313194345	23.8392676291013	24.79510747814805	25.1558017608072
23	24.5433316733738	23.602403800858692	25.554785394150663	26.29947913161684
24	25.12595009374752	24.716031835122816	24.95972154362389	25.198296527505775
25	25.30398036435244	24.164108679836513	25.029730077146773	25.50218087866427
26	24.841502942459172	25.268805625400447	25.428716032498965	24.460975399641413
27	25.625340681969593	24.360594620421402	24.623516981944697	25.3905477156643
28	24.583898027931692	24.377176064934382	25.613335719967107	25.42559018716682
29	24.563656136270644	24.697910610073865	25.513868251609708	25.224565002045786
30	24.56214369922966	24.925980954226624	25.76374197229804	24.74813337424568
31	25.507788421939477	24.62403738227195	24.355540721320654	25.512633474467922
32	25.030971596304003	24.5387234117005	24.284490514717312	26.14581447727819
33	24.618842530282638	24.121130551816957	25.143606998654107	26.116419919246297
34	25.54242197468518	24.405422066210473	25.187424962716904	24.864730996387443
35	26.155032718271553	24.181370203389672	25.25185632361443	24.411740754724345
36	24.778334041094094	25.131720895049288	24.611634510100565	25.478310553756057
37	25.97536051747355	24.789416341574533	24.46754316485519	24.767679976096726
38	24.87692154471873	24.508762863010787	25.171461948636782	25.4428536436337
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	91.0
2	134.0
3	134.0
4	377.5
5	621.0
6	621.0
7	793.0
8	965.0
9	1021.5
10	1078.0
11	1078.0
12	1451.0
13	1824.0
14	2423.0
15	3022.0
16	3022.0
17	4478.0
18	5934.0
19	5934.0
20	7232.0
21	8530.0
22	9159.0
23	9788.0
24	9788.0
25	11582.5
26	13377.0
27	13377.0
28	17184.5
29	20992.0
30	26067.0
31	31142.0
32	31142.0
33	40020.5
34	48899.0
35	48899.0
36	55292.5
37	61686.0
38	70887.0
39	80088.0
40	80088.0
41	85178.5
42	90269.0
43	103325.0
44	116381.0
45	116381.0
46	118540.5
47	120700.0
48	120700.0
49	128202.0
50	135704.0
51	137570.0
52	139436.0
53	139436.0
54	132440.5
55	125445.0
56	125445.0
57	122183.0
58	118921.0
59	106816.5
60	94712.0
61	94712.0
62	91101.5
63	87491.0
64	73578.5
65	59666.0
66	59666.0
67	51140.5
68	42615.0
69	42615.0
70	34164.0
71	25713.0
72	20128.5
73	14544.0
74	14544.0
75	11135.5
76	7727.0
77	7727.0
78	7260.5
79	6794.0
80	5280.0
81	3766.0
82	3766.0
83	3891.0
84	4016.0
85	4016.0
86	2717.5
87	1419.0
88	1209.0
89	999.0
90	999.0
91	726.0
92	453.0
93	374.5
94	296.0
95	296.0
96	270.5
97	245.0
98	245.0
99	485.0
100	725.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12118439069686072
2	0.055377431173523804
3	0.011236975705927672
4	0.0026914911870485445
5	1.3457455935242724E-4
6	4.0372367805728164E-4
7	6.728727967621362E-5
8	6.055855170859225E-4
9	4.0372367805728164E-4
10	6.728727967621361E-4
11	0.0026914911870485445
12	0.003633513102515535
13	0.017427405436139323
14	0.011842561223013595
15	0.027453210107895153
16	0.014130328732004857
17	0.024627144361494182
18	0.007738037162764565
19	0.00968936827337476
20	0.00726702620503107
21	0.008478197239202914
22	0.009554793714022333
23	0.012448146740099518
24	0.017427405436139323
25	0.019917034784159227
26	0.022406664132179132
27	0.011775273943337381
28	0.007468888044059711
29	0.012178997621394664
30	0.00410452406024903
31	0.007603462603412138
32	0.008478197239202914
33	0.011102401146575246
34	0.015947085283262625
35	0.020051609343511655
36	0.01736011815646311
37	0.011842561223013595
38	0.008141760840821848
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1486165.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.74062661580196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03939104509068	41.41288246620199
2	11.925133496682967	12.340277591909322
3	3.416729499906134	5.303511759055171
4	1.464965594709758	3.0319295136349536
5	0.7408551890030491	1.9166155855293072
6	0.4802691353989197	1.4909655605861722
7	0.31547906129989295	1.142615902107502
8	0.24864646364657056	1.0292099067901426
9	0.18064331025836752	0.8411938260048595
>10	0.9927520581864228	9.381609868563396
>50	0.08987914551218293	3.270284840653466
>100	0.08658160574699482	9.78057874957837
>500	0.013059017173454032	4.608419681802444
>1k	0.005615377384585234	4.449904747582918
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3634	0.24452197434336026	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2433	0.16370995145222772	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2388	0.1606820238667981	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2382	0.1602783001887408	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2121	0.14271632019324906	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2092	0.14076498908263887	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1989	0.13383439927598886	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1982	0.13336338831825537	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1979	0.13316152647922672	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1946	0.13094104624991168	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1932	0.1299990243344447	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1692	0.11385007721215343	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1642	0.11048571322834273	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1583	0.10651576372744614	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1569	0.10557374181197915	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1551	0.10436257077780732	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1530	0.10294953790460683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.72872796762136E-5
2	0.0	0.0	0.0	6.72872796762136E-5	6.72872796762136E-5
3	0.0	0.0	0.0	1.345745593524272E-4	6.72872796762136E-5
4	0.0	0.0	0.0	1.345745593524272E-4	6.72872796762136E-5
5	0.0	0.0	0.0	1.345745593524272E-4	1.345745593524272E-4
6	0.0	0.0	0.0	1.345745593524272E-4	1.345745593524272E-4
7	6.72872796762136E-5	0.0	0.0	1.345745593524272E-4	1.345745593524272E-4
8	6.72872796762136E-5	0.0	0.0	2.0186183902864082E-4	1.345745593524272E-4
9	6.72872796762136E-5	0.0	0.0	2.691491187048544E-4	1.345745593524272E-4
10	6.72872796762136E-5	0.0	0.0	2.691491187048544E-4	1.345745593524272E-4
11	6.72872796762136E-5	0.0	0.0	2.691491187048544E-4	1.345745593524272E-4
12	6.72872796762136E-5	0.0	0.0	2.691491187048544E-4	4.7101095773349527E-4
13	6.72872796762136E-5	0.0	0.0	2.691491187048544E-4	5.382982374097089E-4
14	6.72872796762136E-5	0.0	0.0	3.3643639838106806E-4	5.382982374097089E-4
15	6.72872796762136E-5	0.0	0.0	3.3643639838106806E-4	6.055855170859225E-4
16	6.72872796762136E-5	0.0	0.0	4.0372367805728164E-4	6.055855170859225E-4
17	6.72872796762136E-5	0.0	0.0	4.0372367805728164E-4	6.055855170859225E-4
18	6.72872796762136E-5	0.0	6.72872796762136E-5	4.7101095773349527E-4	6.055855170859225E-4
19	6.72872796762136E-5	0.0	6.72872796762136E-5	4.7101095773349527E-4	6.055855170859225E-4
20	1.345745593524272E-4	0.0	6.72872796762136E-5	4.7101095773349527E-4	6.728727967621361E-4
21	1.345745593524272E-4	0.0	6.72872796762136E-5	5.382982374097089E-4	6.728727967621361E-4
22	1.345745593524272E-4	0.0	6.72872796762136E-5	8.074473561145633E-4	6.728727967621361E-4
23	1.345745593524272E-4	0.0	6.72872796762136E-5	0.0013457455935242722	6.728727967621361E-4
24	1.345745593524272E-4	0.0	6.72872796762136E-5	0.0018167565512577674	6.728727967621361E-4
25	1.345745593524272E-4	0.0	6.72872796762136E-5	0.0018167565512577674	6.728727967621361E-4
26	1.345745593524272E-4	0.0	6.72872796762136E-5	0.0020859056699626217	6.728727967621361E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	50	9.693777E-7	25.595638	6
ATCGTTT	445	0.0	18.336424	29
CGTCTAT	70	1.7682783E-5	18.309088	1
TATGCCG	45	0.008860147	17.774752	5
CGTTTAT	470	0.0	17.701496	31
TCTAGCG	535	0.0	17.643635	28
AGAACCG	330	0.0	17.451572	5
CGCGGTC	305	0.0	17.310858	10
TAGACCG	65	2.1036612E-4	17.227835	5
CATCGTT	480	0.0	16.998821	28
CTAGCGG	560	0.0	16.85654	29
TCGTTTA	500	0.0	16.639404	30
CGGCATC	495	0.0	16.161037	25
GCATCGT	500	0.0	15.998889	27
TAGCGGC	595	0.0	15.864979	30
CAAGACG	605	0.0	15.601173	4
ACCGTCG	330	0.0	15.514073	8
AACCGCG	355	0.0	15.322365	7
ATACCGT	335	0.0	15.280979	6
CGTCGTA	325	0.0	15.260992	10
>>END_MODULE