FastQCFastQC Report
Thu 2 Feb 2017
SRR4062856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486165
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39050.26275682713561416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28170.18954826684789372No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23750.15980728923100734No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23650.1591344164342452No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22530.15159824111050926No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21650.14567696049900244No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21500.14466765130385925No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21020.14143786187940102No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20980.14116871276069615No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18680.12569263843516704No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18330.12333758364649955No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17990.12104981613750827No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17080.11492667368697286No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16910.11378278993247722No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16630.11189874610154323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16100.10833252027870391No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15600.10496815629489323No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15110.10167107959075876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1650.018.4257534
CGCGCCA450.00884899417.77863710
CGGACGA450.00885414117.77684416
TCTAGCG4550.017.22986428
CATCGTT4300.017.1153828
ATCGTTT4400.017.09001229
CGGTCCA5100.016.00077210
CTATTAG703.6978401E-415.9996981
AGCGCTA703.6987875E-415.9991614
CTAGCGG4850.015.83421829
CGCCGGT5250.015.5457017
GTATCAA30950.015.301811
TAGCGGC5100.015.05803230
AGGCCCG5550.014.99171610
TTAGGAC2150.014.8849423
TCGTTTA5000.014.71922630
GTATTAC1205.420461E-714.6663891
GCATCGT5150.014.60117327
TCAATAG1103.5408902E-614.5466483
CCGGTCC6000.014.4016679
Produced by FastQC (version 0.11.5)