##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062856_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1486165
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229064740456142	32.0	32.0	32.0	32.0	32.0
2	31.287964660720714	32.0	32.0	32.0	32.0	32.0
3	31.37574630004071	32.0	32.0	32.0	32.0	32.0
4	31.47072498679487	32.0	32.0	32.0	32.0	32.0
5	31.396083880322845	32.0	32.0	32.0	32.0	32.0
6	34.92800866660162	36.0	36.0	36.0	36.0	36.0
7	34.948068350418694	36.0	36.0	36.0	36.0	36.0
8	34.87665030464316	36.0	36.0	36.0	32.0	36.0
9	34.99546214585864	36.0	36.0	36.0	36.0	36.0
10	34.839947112198175	36.0	36.0	36.0	32.0	36.0
11	35.01496536387279	36.0	36.0	36.0	36.0	36.0
12	34.91522812069992	36.0	36.0	36.0	32.0	36.0
13	34.973910030178345	36.0	36.0	36.0	36.0	36.0
14	34.91207974888387	36.0	36.0	36.0	32.0	36.0
15	34.88403373784203	36.0	36.0	36.0	32.0	36.0
16	34.89285913744436	36.0	36.0	36.0	32.0	36.0
17	34.85883465160329	36.0	36.0	36.0	32.0	36.0
18	34.85984732516241	36.0	36.0	36.0	32.0	36.0
19	34.862141148526575	36.0	36.0	36.0	32.0	36.0
20	34.84090191869678	36.0	36.0	36.0	32.0	36.0
21	34.83709884164948	36.0	36.0	36.0	32.0	36.0
22	34.82131930169261	36.0	36.0	36.0	32.0	36.0
23	34.75828390521914	36.0	36.0	36.0	32.0	36.0
24	34.73582408413601	36.0	36.0	36.0	32.0	36.0
25	34.71923709682303	36.0	36.0	36.0	32.0	36.0
26	34.659535785057514	36.0	36.0	36.0	32.0	36.0
27	34.6438484286738	36.0	36.0	36.0	32.0	36.0
28	34.618338475202954	36.0	36.0	36.0	32.0	36.0
29	34.5779095860823	36.0	36.0	36.0	32.0	36.0
30	34.550819727284654	36.0	36.0	36.0	32.0	36.0
31	34.553656558995804	36.0	36.0	36.0	32.0	36.0
32	34.529349029212774	36.0	36.0	36.0	32.0	36.0
33	34.490191869678	36.0	36.0	36.0	32.0	36.0
34	34.48285082746532	36.0	36.0	36.0	32.0	36.0
35	34.458643555729005	36.0	36.0	36.0	32.0	36.0
36	34.431157374854074	36.0	36.0	36.0	32.0	36.0
37	34.428490107087704	36.0	36.0	36.0	32.0	36.0
38	33.98684197245932	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	27.0
22	89.0
23	312.0
24	979.0
25	2371.0
26	4986.0
27	9669.0
28	17017.0
29	26978.0
30	40683.0
31	59674.0
32	85905.0
33	137592.0
34	338862.0
35	761013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08175994821069	18.395836936355543	11.8771538221869	26.645249293246863
2	16.163761199975237	20.367469539027994	36.32041954992854	27.148349711068224
3	17.935828121372797	24.140859191274185	28.725949002970737	29.19736368438229
4	12.078384614297663	15.720121467487663	35.86305368402708	36.338440234187594
5	14.401400655783592	36.84318186253037	32.892735040147066	15.862682441538972
6	34.84788639708133	35.77409954116619	16.060461902124874	13.317552159627605
7	30.479113313353455	30.43207940991628	20.56229401639929	18.526513260330972
8	28.549597979704185	32.66737051339765	18.99720008962943	19.78583141726874
9	27.469304233785092	13.772132342204552	18.47627071085251	40.282292713157844
10	15.999025672323105	26.280806888658166	30.87495029112077	26.84521714789796
11	38.29168012489234	20.961321059431526	21.703205749354005	19.043793066322138
12	24.844279993890297	23.662846757518196	28.128379092171034	23.364494156420466
13	29.588247841250038	18.938055836480704	25.210592930375647	26.263103391893612
14	23.834096483230326	19.608186170445407	24.591011092308054	31.96670625401621
15	25.3973818519478	27.076266767149004	21.927309551765788	25.59904182913741
16	26.24271194652007	25.566609360333477	23.163309592138155	25.0273691010083
17	24.223084247038518	25.672923262221893	24.836205939448178	25.26778655129141
18	25.540569183098782	24.234859520981857	25.867518075045503	24.35705322087386
19	25.908025017410587	24.624183721188427	25.158848445495625	24.308942815905368
20	26.103494564869983	23.815525194039694	24.629835852681232	25.45114438840909
21	27.465273011592263	23.740583138872964	24.08395035810314	24.71019349143163
22	26.107733663489586	23.90979467286607	24.808685442060604	25.17378622158374
23	24.40967187358066	23.551960919547966	25.624880144533073	26.413487062338298
24	25.081400786588297	24.730295761237816	25.013238772276296	25.17506467989759
25	25.292010757919233	24.065831360128545	25.061887642052238	25.58027023989998
26	24.93336877130063	25.179236491237518	25.481961962500797	24.40543277496106
27	25.68897220560598	24.26604618739667	24.625697181264776	25.419284425732574
28	24.541353079907008	24.314056649160758	25.650314736250685	25.49427553468155
29	24.660922575891643	24.521301470563497	25.560418930603262	25.257357022941594
30	24.53274030810845	24.79933251018561	25.817321764407048	24.850605417298887
31	25.48162552610242	24.537113981287405	24.37784499029381	25.603415502316363
32	25.03288665794175	24.42474422422813	24.48254399747	26.05982512036012
33	24.57264166495645	24.076936275581783	25.210054065329217	26.140367994132554
34	25.493266225486405	24.245221762051994	25.368246459848	24.893265552613606
35	26.09838073161459	24.149404675793065	25.262740005315692	24.48947458727665
36	24.752164127132588	25.042172302537068	24.715223410590344	25.490440159740004
37	26.004851412864653	24.62647148869742	24.600094875064343	24.768582223373585
38	24.942216970816794	24.250368230133574	25.227380845842617	25.580033953207014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	46.5
2	67.0
3	67.0
4	276.0
5	485.0
6	485.0
7	704.0
8	923.0
9	1022.5
10	1122.0
11	1122.0
12	1404.0
13	1686.0
14	2411.5
15	3137.0
16	3137.0
17	4696.5
18	6256.0
19	6256.0
20	7408.5
21	8561.0
22	9223.0
23	9885.0
24	9885.0
25	11477.5
26	13070.0
27	13070.0
28	17060.5
29	21051.0
30	25825.5
31	30600.0
32	30600.0
33	39955.5
34	49311.0
35	49311.0
36	55621.0
37	61931.0
38	71296.5
39	80662.0
40	80662.0
41	85775.0
42	90888.0
43	103714.0
44	116540.0
45	116540.0
46	118611.5
47	120683.0
48	120683.0
49	128740.5
50	136798.0
51	138176.5
52	139555.0
53	139555.0
54	133025.5
55	126496.0
56	126496.0
57	122945.0
58	119394.0
59	106938.0
60	94482.0
61	94482.0
62	91092.5
63	87703.0
64	73724.0
65	59745.0
66	59745.0
67	50894.5
68	42044.0
69	42044.0
70	33831.5
71	25619.0
72	20034.5
73	14450.0
74	14450.0
75	10869.5
76	7289.0
77	7289.0
78	7037.5
79	6786.0
80	5248.0
81	3710.0
82	3710.0
83	3572.0
84	3434.0
85	3434.0
86	2192.0
87	950.0
88	792.0
89	634.0
90	634.0
91	380.0
92	126.0
93	84.5
94	43.0
95	43.0
96	26.0
97	9.0
98	9.0
99	11.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00968936827337476
2	6.728727967621362E-5
3	0.0
4	4.0372367805728164E-4
5	2.691491187048545E-4
6	4.0372367805728164E-4
7	2.0186183902864082E-4
8	0.0030952148651058266
9	0.003229789424458253
10	8.074473561145633E-4
11	0.0057194187724781565
12	2.691491187048545E-4
13	2.691491187048545E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	6.728727967621362E-5
22	0.0
23	0.0
24	0.0
25	2.691491187048545E-4
26	0.0
27	1.3457455935242724E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.3457455935242724E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1486165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.516581170847836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26924323741304	38.75625335946196
2	12.921147359066666	12.796220840514211
3	3.761768887253536	5.58809803354979
4	1.613471494355713	3.1957436886845527
5	0.8395345422347437	2.0785440153148635
6	0.501348859664059	1.4895048902680426
7	0.3437882285690438	1.1916252407864731
8	0.26024042295722116	1.0308972821837612
9	0.20119436393827264	0.896621134775992
>10	1.0797911241982645	9.82629800574465
>50	0.09381316277856908	3.2756436179310193
>100	0.09488969000267787	10.34098392214353
>500	0.013497200891385912	4.690633271725901
>1k	0.006271426676805575	4.842932696915356
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3905	0.26275682713561416	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2817	0.18954826684789372	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2375	0.15980728923100734	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2365	0.1591344164342452	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2253	0.15159824111050926	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2165	0.14567696049900244	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2150	0.14466765130385925	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2102	0.14143786187940102	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2098	0.14116871276069615	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1868	0.12569263843516704	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1833	0.12333758364649955	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1799	0.12104981613750827	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1708	0.11492667368697286	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1691	0.11378278993247722	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1663	0.11189874610154323	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1610	0.10833252027870391	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1560	0.10496815629489323	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1511	0.10167107959075876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.72872796762136E-5
2	0.0	0.0	0.0	0.0	1.345745593524272E-4
3	0.0	0.0	0.0	0.0	1.345745593524272E-4
4	0.0	0.0	0.0	0.0	1.345745593524272E-4
5	0.0	0.0	0.0	0.0	1.345745593524272E-4
6	0.0	0.0	0.0	0.0	1.345745593524272E-4
7	0.0	0.0	0.0	0.0	1.345745593524272E-4
8	0.0	0.0	0.0	0.0	1.345745593524272E-4
9	0.0	0.0	0.0	6.72872796762136E-5	1.345745593524272E-4
10	0.0	0.0	0.0	6.72872796762136E-5	1.345745593524272E-4
11	0.0	0.0	0.0	6.72872796762136E-5	1.345745593524272E-4
12	0.0	0.0	0.0	6.72872796762136E-5	3.3643639838106806E-4
13	0.0	0.0	0.0	6.72872796762136E-5	4.0372367805728164E-4
14	0.0	0.0	0.0	2.0186183902864082E-4	4.0372367805728164E-4
15	0.0	0.0	0.0	2.0186183902864082E-4	6.728727967621361E-4
16	0.0	0.0	0.0	3.3643639838106806E-4	6.728727967621361E-4
17	0.0	0.0	0.0	3.3643639838106806E-4	6.728727967621361E-4
18	0.0	0.0	0.0	4.0372367805728164E-4	6.728727967621361E-4
19	0.0	0.0	0.0	4.0372367805728164E-4	6.728727967621361E-4
20	0.0	0.0	0.0	4.0372367805728164E-4	8.074473561145633E-4
21	0.0	0.0	0.0	4.7101095773349527E-4	8.074473561145633E-4
22	0.0	0.0	0.0	7.401600764383497E-4	8.074473561145633E-4
23	0.0	0.0	0.0	0.0011438837544956314	8.074473561145633E-4
24	0.0	0.0	0.0	0.0016148947122291266	8.074473561145633E-4
25	0.0	0.0	0.0	0.0016148947122291266	8.074473561145633E-4
26	0.0	0.0	0.0	0.0018167565512577674	8.074473561145633E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	165	0.0	18.425753	4
CGCGCCA	45	0.008848994	17.778637	10
CGGACGA	45	0.008854141	17.776844	16
TCTAGCG	455	0.0	17.229864	28
CATCGTT	430	0.0	17.11538	28
ATCGTTT	440	0.0	17.090012	29
CGGTCCA	510	0.0	16.000772	10
CTATTAG	70	3.6978401E-4	15.999698	1
AGCGCTA	70	3.6987875E-4	15.99916	14
CTAGCGG	485	0.0	15.834218	29
CGCCGGT	525	0.0	15.545701	7
GTATCAA	3095	0.0	15.30181	1
TAGCGGC	510	0.0	15.058032	30
AGGCCCG	555	0.0	14.991716	10
TTAGGAC	215	0.0	14.884942	3
TCGTTTA	500	0.0	14.719226	30
GTATTAC	120	5.420461E-7	14.666389	1
GCATCGT	515	0.0	14.601173	27
TCAATAG	110	3.5408902E-6	14.546648	3
CCGGTCC	600	0.0	14.401667	9
>>END_MODULE