FastQCFastQC Report
Thu 2 Feb 2017
SRR4062855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189695
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29050.2441802310676266No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20230.1700435825989014No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20060.16861464493000305No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19170.16113373595753536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18850.1584439709337267No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18770.15777152967777455No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18710.15726719873581044No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18280.1536528269850676No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18100.15213983415917526No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18030.15155144806021711No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17500.14709652473953408No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17330.14566758707063573No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15930.13389986509147303No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15390.12936088661379597No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15230.12801600410189165No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14930.12549434939207108No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14810.12448568750814284No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14590.12263647405427441No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT14410.12112348122838205No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14330.1204510399724299No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14250.11977859871647775No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13200.11095280723210571No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13140.11044847629014158No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13130.11036442113314757No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13000.10927170409222532No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12740.10708627001038082No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC12530.10532111171350642No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT12340.10372406373062003No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12100.10170673996276357No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG12070.10145457449178151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG200.003755162231.9972275
TAGGACG852.399429E-718.8211064
ATCGTTT3450.018.08690829
CATCGTT3450.017.622428
CTAATTC652.1018725E-417.2292773
TATTAGC2000.016.8027822
GTATTAC1252.8285285E-916.6511551
TCTAGAT3100.016.5188642
AACCGCG2950.016.2697777
GTATTAG3250.016.2570461
AAGACGG4750.016.167025
GTATAGA1102.004017E-716.0107251
CTTATAC600.00244338715.9986123
ACCGTCG3700.015.9986128
TACCGTC3700.015.9986127
GGCTAGT703.6989743E-415.9986126
TCGTTTA3750.015.78662530
CCGTCGT4000.015.5999599
GGCATCG4000.015.59995926
CGTCGTA3800.015.57890710
Produced by FastQC (version 0.11.5)