##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062855_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1189695
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.274839349581196	32.0	32.0	32.0	32.0	32.0
2	30.931629535301063	32.0	32.0	32.0	32.0	32.0
3	30.980970753008123	32.0	32.0	32.0	32.0	32.0
4	31.036208439978314	32.0	32.0	32.0	32.0	32.0
5	30.968618847687853	32.0	32.0	32.0	32.0	32.0
6	34.632204052299116	36.0	36.0	36.0	32.0	36.0
7	34.552745871841104	36.0	36.0	36.0	32.0	36.0
8	34.5447681968908	36.0	36.0	36.0	32.0	36.0
9	34.6568507054329	36.0	36.0	36.0	32.0	36.0
10	34.4047844195361	36.0	36.0	36.0	32.0	36.0
11	34.651772933398895	36.0	36.0	36.0	32.0	36.0
12	34.48003816104128	36.0	36.0	36.0	32.0	36.0
13	34.56472709391903	36.0	36.0	36.0	32.0	36.0
14	34.457141536276104	36.0	36.0	36.0	32.0	36.0
15	34.41745405334981	36.0	36.0	36.0	32.0	36.0
16	34.43337325953291	36.0	36.0	36.0	32.0	36.0
17	34.34244407179992	36.0	36.0	36.0	32.0	36.0
18	34.38608046600179	36.0	36.0	36.0	32.0	36.0
19	34.36839273931554	36.0	36.0	36.0	32.0	36.0
20	34.34647535712935	36.0	36.0	36.0	32.0	36.0
21	34.32387208486209	36.0	36.0	36.0	32.0	36.0
22	34.312325427945815	36.0	36.0	36.0	32.0	36.0
23	34.24985479471629	36.0	36.0	36.0	32.0	36.0
24	34.22598481123313	36.0	36.0	36.0	32.0	36.0
25	34.215563652869015	36.0	36.0	36.0	32.0	36.0
26	34.172297941909484	36.0	36.0	36.0	32.0	36.0
27	34.15946440053963	36.0	36.0	36.0	32.0	36.0
28	34.14944502582595	36.0	36.0	36.0	32.0	36.0
29	34.10643063978583	36.0	36.0	36.0	32.0	36.0
30	34.090073506234795	36.0	36.0	36.0	32.0	36.0
31	34.10089728880091	36.0	36.0	36.0	32.0	36.0
32	34.064590504288915	36.0	36.0	36.0	32.0	36.0
33	34.01527534368052	36.0	36.0	36.0	32.0	36.0
34	34.02888135194315	36.0	36.0	36.0	32.0	36.0
35	33.972834213811105	36.0	36.0	36.0	32.0	36.0
36	33.93938278298219	36.0	36.0	36.0	32.0	36.0
37	33.9342234774459	36.0	36.0	36.0	32.0	36.0
38	33.3332055694947	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	6.0
10	15.0
11	12.0
12	5.0
13	9.0
14	185.0
15	311.0
16	396.0
17	483.0
18	613.0
19	764.0
20	1049.0
21	1446.0
22	2117.0
23	3252.0
24	4822.0
25	7286.0
26	10547.0
27	15159.0
28	21725.0
29	29617.0
30	39751.0
31	53773.0
32	73858.0
33	111486.0
34	253056.0
35	557947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53573382211607	17.307352443661227	11.377579616547921	25.77933411767478
2	16.796032319406027	20.04008386830355	37.20255641892773	25.961327393362698
3	18.375391537247108	24.480178085347134	28.727022669228997	28.41740770817676
4	12.366264760470184	15.589611856793178	36.56687938779353	35.47724399494311
5	14.599829199490289	36.5746764708849	33.496316693732496	15.329177635892316
6	33.67370182677687	35.94614063651822	17.111333951421	13.26882358528391
7	29.958770945494432	30.672483283530656	21.432467985492078	17.936277785482837
8	28.306449579302175	32.134841849557446	19.926871706074692	19.631836865065686
9	27.856332321865363	14.057611646731502	18.759929057149343	39.326126974253796
10	16.1024977199847	26.019240387161307	31.2725637458655	26.605698146988487
11	37.64986336512655	21.099685878485325	22.221764577818835	19.0286861785693
12	25.085717984259325	23.980273305667097	28.320852144777863	22.61315656529571
13	29.295825798787916	19.08305948360727	25.411993567158547	26.209121150446276
14	23.980847198743096	20.184080074580255	24.780197563700465	31.05487516297618
15	25.390758665800135	26.876780809421756	22.42686963445116	25.30559089032694
16	26.08373649191091	25.562621630596063	23.583242613896676	24.770399263596357
17	24.12520419118432	25.630943403206025	25.185358935103565	25.058493470506093
18	25.493874950299382	24.014078409714674	26.246970678946184	24.24507596103976
19	25.759960053866752	24.97894253708383	25.342761696769173	23.918335712280243
20	25.879253574890072	23.75209836443158	24.914741467211716	25.453906593466634
21	27.676998291885784	23.927637153501635	23.920912294387435	24.47445226022515
22	26.108542093454705	24.067104739984686	24.752207248972976	25.072145917587633
23	24.203306564624448	23.817453825868608	25.4963755902328	26.482864019274142
24	24.82512103448653	25.209556323174482	24.92179730432933	25.043525338009665
25	24.991950338422065	24.253651351712623	25.06399796215356	25.69040034771175
26	24.728450029761202	25.30988643662545	25.556467260993994	24.405196272619357
27	25.745473192219105	24.55530523377999	24.38179861799963	25.31742295600128
28	24.44186286962216	24.33359363901458	25.803129888612148	25.421413602751112
29	24.47503654715739	24.760436430643626	25.700868977495023	25.063658044703963
30	24.43815313437252	24.87323981547483	26.14058948529313	24.548017564859524
31	25.246486480125153	24.865608232551768	24.21565223823762	25.672253049085462
32	24.625991814144598	24.84043129992695	24.253517740643385	26.280059145285062
33	24.25668166893354	24.243147685184407	25.29266189082362	26.207508755058434
34	25.30841313194744	24.43800507247656	25.50066957699379	24.752912218582214
35	26.427409490422566	24.25854234709104	25.221119531910063	24.092928630576328
36	24.599145370279295	25.22485941820279	24.70700391165856	25.468991299859354
37	25.89242181221481	25.102894150164936	24.669797204372664	24.334886833247587
38	24.639591399621054	24.730796841349605	25.32964252510461	25.299969233924724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	241.0
1	231.0
2	221.0
3	221.0
4	516.5
5	812.0
6	812.0
7	961.5
8	1111.0
9	1075.0
10	1039.0
11	1039.0
12	1359.0
13	1679.0
14	2114.5
15	2550.0
16	2550.0
17	3756.0
18	4962.0
19	4962.0
20	6136.5
21	7311.0
22	8070.0
23	8829.0
24	8829.0
25	10195.5
26	11562.0
27	11562.0
28	14971.5
29	18381.0
30	22413.0
31	26445.0
32	26445.0
33	34402.0
34	42359.0
35	42359.0
36	46628.5
37	50898.0
38	57959.0
39	65020.0
40	65020.0
41	68762.5
42	72505.0
43	82919.5
44	93334.0
45	93334.0
46	95808.0
47	98282.0
48	98282.0
49	104362.5
50	110443.0
51	110677.0
52	110911.0
53	110911.0
54	104375.0
55	97839.0
56	97839.0
57	96173.5
58	94508.0
59	83926.5
60	73345.0
61	73345.0
62	71438.0
63	69531.0
64	57204.5
65	44878.0
66	44878.0
67	37498.0
68	30118.0
69	30118.0
70	24444.0
71	18770.0
72	14708.5
73	10647.0
74	10647.0
75	8045.5
76	5444.0
77	5444.0
78	5544.0
79	5644.0
80	4334.0
81	3024.0
82	3024.0
83	3366.5
84	3709.0
85	3709.0
86	2414.5
87	1120.0
88	974.0
89	828.0
90	828.0
91	595.0
92	362.0
93	310.5
94	259.0
95	259.0
96	234.5
97	210.0
98	210.0
99	387.0
100	564.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12078726060040598
2	0.057829948011885395
3	0.012860439020084978
4	0.0026057098668146035
5	2.521654709820584E-4
6	3.362206279760779E-4
7	0.0
8	4.2027578497009734E-4
9	4.2027578497009734E-4
10	8.405515699401947E-4
11	0.002353544395832545
12	0.0031940959657727397
13	0.014625597316959388
14	0.008741736327378026
15	0.023367333644337414
16	0.010927170409222533
17	0.020677568620528792
18	0.006051971303569402
19	0.007817129600443812
20	0.00655630224553352
21	0.006640357402527539
22	0.007733074443449791
23	0.010254729153270376
24	0.013280714805055077
25	0.017147252026779972
26	0.019080520637642418
27	0.00966634305431224
28	0.005715750675593325
29	0.01143150135118665
30	0.003950592378718916
31	0.006388191931545479
32	0.006892522873509598
33	0.008153350228419889
34	0.011599611665174687
35	0.015129928258923505
36	0.014625597316959388
37	0.010338784310264396
38	0.006136026460563422
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1189695.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.11881943515233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.03990193562665	43.57862737396156
2	11.629550816871866	12.354960199066898
3	2.993434172586165	4.770230679138574
4	1.1677863620560704	2.4812573161955935
5	0.5561557654727456	1.4771168841982854
6	0.3292204627790014	1.049268139002904
7	0.21219791239071428	0.7890191814959038
8	0.14509902616796086	0.6165991176985886
9	0.10041678409958284	0.4800618920559975
>10	0.6081529890460924	6.165047238778963
>50	0.08902701332462809	3.3694200296835612
>100	0.10791815423037826	12.491328882107602
>500	0.015257940702388968	5.649409767525687
>1k	0.005880664645712415	4.727653299089956
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2905	0.2441802310676266	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2023	0.1700435825989014	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2006	0.16861464493000305	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1917	0.16113373595753536	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1885	0.1584439709337267	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1877	0.15777152967777455	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1871	0.15726719873581044	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1828	0.1536528269850676	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1810	0.15213983415917526	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1803	0.15155144806021711	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1750	0.14709652473953408	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1733	0.14566758707063573	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1593	0.13389986509147303	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1539	0.12936088661379597	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1523	0.12801600410189165	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1493	0.12549434939207108	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1481	0.12448568750814284	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1459	0.12263647405427441	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1441	0.12112348122838205	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1433	0.1204510399724299	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1425	0.11977859871647775	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1320	0.11095280723210571	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1314	0.11044847629014158	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1313	0.11036442113314757	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1300	0.10927170409222532	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1274	0.10708627001038082	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1253	0.10532111171350642	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1234	0.10372406373062003	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1210	0.10170673996276357	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1207	0.10145457449178151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6811031398803895E-4
2	0.0	0.0	0.0	0.0	1.6811031398803895E-4
3	0.0	0.0	0.0	0.0	1.6811031398803895E-4
4	0.0	0.0	0.0	0.0	1.6811031398803895E-4
5	0.0	0.0	0.0	0.0	1.6811031398803895E-4
6	0.0	0.0	0.0	0.0	1.6811031398803895E-4
7	0.0	0.0	0.0	0.0	1.6811031398803895E-4
8	0.0	0.0	0.0	0.0	1.6811031398803895E-4
9	0.0	0.0	0.0	0.0	1.6811031398803895E-4
10	0.0	0.0	0.0	0.0	1.6811031398803895E-4
11	0.0	0.0	0.0	0.0	1.6811031398803895E-4
12	0.0	0.0	0.0	0.0	3.362206279760779E-4
13	0.0	0.0	0.0	0.0	5.043309419641168E-4
14	0.0	0.0	0.0	8.405515699401948E-5	5.043309419641168E-4
15	0.0	0.0	0.0	8.405515699401948E-5	6.724412559521558E-4
16	0.0	0.0	0.0	8.405515699401948E-5	7.564964129461752E-4
17	0.0	0.0	0.0	8.405515699401948E-5	7.564964129461752E-4
18	0.0	0.0	0.0	2.521654709820584E-4	8.405515699401948E-4
19	0.0	0.0	0.0	2.521654709820584E-4	8.405515699401948E-4
20	0.0	0.0	0.0	2.521654709820584E-4	0.0010086618839282337
21	0.0	0.0	0.0	3.362206279760779E-4	0.0010086618839282337
22	0.0	0.0	0.0	5.883860989581364E-4	0.0010927170409222532
23	8.405515699401948E-5	0.0	0.0	8.405515699401948E-4	0.0010927170409222532
24	8.405515699401948E-5	0.0	0.0	0.0015129928258923505	0.0010927170409222532
25	8.405515699401948E-5	0.0	0.0	0.0021854340818445064	0.0010927170409222532
26	8.405515699401948E-5	0.0	0.0	0.0027738201808026426	0.0014289376688983312
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	20	0.0037551622	31.997227	5
TAGGACG	85	2.399429E-7	18.821106	4
ATCGTTT	345	0.0	18.086908	29
CATCGTT	345	0.0	17.6224	28
CTAATTC	65	2.1018725E-4	17.229277	3
TATTAGC	200	0.0	16.802782	2
GTATTAC	125	2.8285285E-9	16.651155	1
TCTAGAT	310	0.0	16.518864	2
AACCGCG	295	0.0	16.269777	7
GTATTAG	325	0.0	16.257046	1
AAGACGG	475	0.0	16.16702	5
GTATAGA	110	2.004017E-7	16.010725	1
CTTATAC	60	0.002443387	15.998612	3
ACCGTCG	370	0.0	15.998612	8
TACCGTC	370	0.0	15.998612	7
GGCTAGT	70	3.6989743E-4	15.998612	6
TCGTTTA	375	0.0	15.786625	30
CCGTCGT	400	0.0	15.599959	9
GGCATCG	400	0.0	15.599959	26
CGTCGTA	380	0.0	15.578907	10
>>END_MODULE