FastQCFastQC Report
Thu 2 Feb 2017
SRR4062855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189695
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34050.2862078095646363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24000.20173237678564673No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21590.18147508395008805No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20330.17088413416884157No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20140.16928708618595523No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19300.1622264529984576No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19210.16146995658551141No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18880.15869613640470875No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18800.1580236951487566No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18610.15642664716587024No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18170.1527282202581334No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17620.14810518662346234No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16960.14255754626185702No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15960.1341520305624551No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15670.1317144310096285No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15500.1302854933407302No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15320.12877250051483785No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14950.1256624597060591No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14740.1238973014091847No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG14160.11902210230353158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14120.1186858816755555No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13990.11759316463463325No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13830.11624828212272893No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT13470.11322229647094423No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13460.11313824131395023No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13150.11053253144713562No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12750.10717032516737483No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC12650.10632977359743463No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG12570.10565733234148247No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT12520.10523705655651237No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG12150.10212701574773367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC552.2180848E-623.27343
TCTAGCG4050.019.3577728
CTAGCGG4100.019.121729
AGCGGCG4400.017.8179531
TTCTACG450.00884897917.778292
TAGCGGC4450.017.61774630
CAAGACG3700.016.4329074
GTTTTCG5400.016.29608228
AAGACGG3800.016.0004635
ATACCGT4800.016.0004636
TACCGTC4500.016.0004627
ACCGTCG4400.015.999798
GTATTAG3450.015.770561
TTTTCGG5650.015.57501729
TTCGGAA5700.015.43839331
CGCAAGA4050.015.4078532
AGGCCCG5850.015.31603810
TTTCGGA5750.015.30414730
GTGTAAT1052.1814649E-615.2404571
CGGCATC4000.015.199825
Produced by FastQC (version 0.11.5)