##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1189695
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24189897410681	32.0	32.0	32.0	32.0	32.0
2	31.33166820067328	32.0	32.0	32.0	32.0	32.0
3	31.4094234236506	32.0	32.0	32.0	32.0	32.0
4	31.50740231740068	32.0	32.0	32.0	32.0	32.0
5	31.440101034298706	32.0	32.0	32.0	32.0	32.0
6	34.96710921706824	36.0	36.0	36.0	36.0	36.0
7	34.99557197432956	36.0	36.0	36.0	36.0	36.0
8	34.93861535939884	36.0	36.0	36.0	36.0	36.0
9	35.04095167248749	36.0	36.0	36.0	36.0	36.0
10	34.88820916285266	36.0	36.0	36.0	32.0	36.0
11	35.06435262819462	36.0	36.0	36.0	36.0	36.0
12	34.95047638260226	36.0	36.0	36.0	36.0	36.0
13	35.012693169257666	36.0	36.0	36.0	36.0	36.0
14	34.960605029020044	36.0	36.0	36.0	32.0	36.0
15	34.93200778350754	36.0	36.0	36.0	32.0	36.0
16	34.936109675168844	36.0	36.0	36.0	36.0	36.0
17	34.89796124216711	36.0	36.0	36.0	32.0	36.0
18	34.897730090485375	36.0	36.0	36.0	32.0	36.0
19	34.891144368934896	36.0	36.0	36.0	32.0	36.0
20	34.882864095419414	36.0	36.0	36.0	32.0	36.0
21	34.864964549737536	36.0	36.0	36.0	32.0	36.0
22	34.85140140960498	36.0	36.0	36.0	32.0	36.0
23	34.79705722895364	36.0	36.0	36.0	32.0	36.0
24	34.76256099252329	36.0	36.0	36.0	32.0	36.0
25	34.7520608223116	36.0	36.0	36.0	32.0	36.0
26	34.683854265168804	36.0	36.0	36.0	32.0	36.0
27	34.66998432371322	36.0	36.0	36.0	32.0	36.0
28	34.63716919042275	36.0	36.0	36.0	32.0	36.0
29	34.5926989690635	36.0	36.0	36.0	32.0	36.0
30	34.57563745329686	36.0	36.0	36.0	32.0	36.0
31	34.57116740004791	36.0	36.0	36.0	32.0	36.0
32	34.5481404897894	36.0	36.0	36.0	32.0	36.0
33	34.4982327403242	36.0	36.0	36.0	32.0	36.0
34	34.49699712951639	36.0	36.0	36.0	32.0	36.0
35	34.44997331248766	36.0	36.0	36.0	32.0	36.0
36	34.413697628383744	36.0	36.0	36.0	32.0	36.0
37	34.417526340784825	36.0	36.0	36.0	32.0	36.0
38	33.97197432955505	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	22.0
22	86.0
23	280.0
24	718.0
25	1764.0
26	3755.0
27	7502.0
28	13043.0
29	21035.0
30	31942.0
31	46383.0
32	66825.0
33	108055.0
34	267187.0
35	621094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77579484834373	18.617381670110408	12.034737785369511	26.572085696176355
2	15.822878973182203	20.994876838181213	36.5382724143583	26.64397177427828
3	17.322927304897473	25.227894544400037	29.03828292125293	28.41089522944957
4	11.84675374235202	16.419277914554428	36.55228701858378	35.18168132450977
5	14.069068122501985	37.16137329315497	33.159591323826696	15.609967260516349
6	33.620718639782396	36.46019305837141	16.793842439892007	13.125245861954186
7	29.652203222859086	30.842346192996654	21.435938299841386	18.06951228430287
8	28.066472494357654	32.49360113981667	19.786830746470645	19.653095619355028
9	27.804657856202603	13.860341647851545	18.608224631529044	39.72677586441681
10	15.937593512226778	26.183591609200423	31.22148402432915	26.65733085424365
11	37.713879345780335	20.970620301589314	22.293551529844127	19.021948822786225
12	24.807891131303112	24.175373416604327	28.291235531945297	22.725499920147264
13	29.296104712640393	19.126178664429677	25.375348619056666	26.202368003873268
14	23.907724248651967	20.117845330105617	24.811569351808657	31.162861069433763
15	25.28479988568499	26.94421679506092	22.34236505995234	25.428618259301754
16	26.07533863721374	25.528391730653656	23.496610475794217	24.899659156338387
17	24.14543223263105	25.783583187287494	25.064911594988633	25.00607298509282
18	25.523684641861987	24.098613510185384	26.21697157674866	24.160730271203963
19	25.7091103181908	25.013974169850258	25.33842707584717	23.938488436111776
20	25.840152307944475	23.658248542693716	25.044065916054116	25.457533233307693
21	27.63275903951692	23.99131540657968	23.96929291842517	24.40663263547823
22	25.917336726923057	24.163608457300782	24.775110238431058	25.143944577345103
23	24.13168775474009	23.616092554970063	25.626022847911184	26.626196842378665
24	24.785344142826524	25.10004665061213	25.08331967437032	25.031289532191025
25	24.99962175147559	24.11813457914388	25.16218456174445	25.72005910763608
26	24.64917478849621	25.214697884751974	25.679691013242888	24.456436313508924
27	25.867177498732868	24.435463602795007	24.40604424839013	25.291314650081997
28	24.531917844489552	24.195697216513476	25.8973938698574	25.37499106913957
29	24.549737537772288	24.62034386964726	25.84721294113197	24.98270565144848
30	24.559487935983594	24.73407049706017	26.16208355923157	24.544358007724668
31	25.274545156531715	24.63370863960931	24.45946229916071	25.632283904698262
32	24.808123090371904	24.652957270560943	24.403481564602693	26.135438074464464
33	24.203346235799934	24.080625706588663	25.472915327037605	26.243112730573802
34	25.206544534523555	24.265715162289496	25.74096722269153	24.786773080495422
35	26.474096301993367	24.04103572764448	25.295727056094208	24.18914091426794
36	24.745249832940374	25.060120451039975	24.865868983226793	25.32876073279286
37	25.940177944767356	24.905206796699993	24.63841572840098	24.516199530131672
38	24.661887846790854	24.40442668450879	25.48226686862336	25.451418600076998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	114.0
1	118.0
2	122.0
3	122.0
4	443.5
5	765.0
6	765.0
7	913.5
8	1062.0
9	1085.0
10	1108.0
11	1108.0
12	1421.5
13	1735.0
14	2248.0
15	2761.0
16	2761.0
17	4076.0
18	5391.0
19	5391.0
20	6466.0
21	7541.0
22	8171.0
23	8801.0
24	8801.0
25	10156.0
26	11511.0
27	11511.0
28	14798.5
29	18086.0
30	22185.5
31	26285.0
32	26285.0
33	34476.5
34	42668.0
35	42668.0
36	46849.5
37	51031.0
38	58395.5
39	65760.0
40	65760.0
41	68850.5
42	71941.0
43	82852.5
44	93764.0
45	93764.0
46	95798.5
47	97833.0
48	97833.0
49	104595.0
50	111357.0
51	111266.5
52	111176.0
53	111176.0
54	104364.0
55	97552.0
56	97552.0
57	96360.0
58	95168.0
59	84235.0
60	73302.0
61	73302.0
62	71830.0
63	70358.0
64	57703.5
65	45049.0
66	45049.0
67	37672.5
68	30296.0
69	30296.0
70	24541.5
71	18787.0
72	14699.0
73	10611.0
74	10611.0
75	7808.5
76	5006.0
77	5006.0
78	5190.0
79	5374.0
80	4177.0
81	2980.0
82	2980.0
83	2978.0
84	2976.0
85	2976.0
86	1862.0
87	748.0
88	635.5
89	523.0
90	523.0
91	304.5
92	86.0
93	59.5
94	33.0
95	33.0
96	29.0
97	25.0
98	25.0
99	17.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008153350228419889
2	0.0
3	0.0
4	1.6811031398803895E-4
5	0.0
6	2.521654709820584E-4
7	5.043309419641168E-4
8	0.002521654709820584
9	0.004118702692706954
10	9.246067269342143E-4
11	0.0053795300476172465
12	4.2027578497009734E-4
13	8.405515699401948E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.6811031398803895E-4
22	8.405515699401948E-5
23	1.6811031398803895E-4
24	0.0
25	8.405515699401948E-5
26	0.0
27	1.6811031398803895E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.405515699401948E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1189695.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.16251412578848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73846739857574	41.30782978774143
2	12.42835003029489	12.717312679704118
3	3.2260128390075486	4.951527823370961
4	1.238994108048875	2.535602142192771
5	0.6249245597148949	1.5986355806982728
6	0.3588347064634933	1.1015331442956984
7	0.2294364015550817	0.821698019487236
8	0.16775973192144264	0.6866407723335437
9	0.11698020723897981	0.5386501354771772
>10	0.6391263609743197	6.246852157413038
>50	0.09301881035264897	3.350238743267528
>100	0.11513410114422512	13.037722246379657
>500	0.015857780517722842	5.6526458720750155
>1k	0.007102964190230025	5.4531108955635545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3405	0.2862078095646363	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2400	0.20173237678564673	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2159	0.18147508395008805	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2033	0.17088413416884157	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2014	0.16928708618595523	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1930	0.1622264529984576	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1921	0.16146995658551141	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1888	0.15869613640470875	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1880	0.1580236951487566	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1861	0.15642664716587024	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1817	0.1527282202581334	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1762	0.14810518662346234	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1696	0.14255754626185702	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1596	0.1341520305624551	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1567	0.1317144310096285	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1550	0.1302854933407302	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1532	0.12877250051483785	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1495	0.1256624597060591	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1474	0.1238973014091847	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1416	0.11902210230353158	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1412	0.1186858816755555	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1399	0.11759316463463325	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1383	0.11624828212272893	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1347	0.11322229647094423	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1346	0.11313824131395023	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1315	0.11053253144713562	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1275	0.10717032516737483	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1265	0.10632977359743463	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1257	0.10565733234148247	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1252	0.10523705655651237	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1215	0.10212701574773367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.405515699401948E-5
2	0.0	0.0	0.0	0.0	8.405515699401948E-5
3	0.0	0.0	0.0	0.0	8.405515699401948E-5
4	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
5	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
6	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
7	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
8	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
9	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
10	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
11	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-5
12	0.0	0.0	0.0	8.405515699401948E-5	4.202757849700974E-4
13	0.0	0.0	0.0	8.405515699401948E-5	5.043309419641168E-4
14	0.0	0.0	0.0	8.405515699401948E-5	5.043309419641168E-4
15	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-4
16	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-4
17	0.0	0.0	0.0	8.405515699401948E-5	8.405515699401948E-4
18	0.0	0.0	0.0	1.6811031398803895E-4	9.246067269342142E-4
19	0.0	0.0	0.0	1.6811031398803895E-4	9.246067269342142E-4
20	0.0	0.0	0.0	1.6811031398803895E-4	0.0011767721979162728
21	0.0	0.0	0.0	2.521654709820584E-4	0.001260827354910292
22	8.405515699401948E-5	0.0	0.0	3.362206279760779E-4	0.0013448825119043116
23	8.405515699401948E-5	0.0	0.0	5.883860989581364E-4	0.0013448825119043116
24	8.405515699401948E-5	0.0	0.0	0.001260827354910292	0.0013448825119043116
25	8.405515699401948E-5	0.0	0.0	0.0020173237678564673	0.0013448825119043116
26	8.405515699401948E-5	0.0	0.0	0.002521654709820584	0.0014289376688983312
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	55	2.2180848E-6	23.2734	3
TCTAGCG	405	0.0	19.35777	28
CTAGCGG	410	0.0	19.1217	29
AGCGGCG	440	0.0	17.81795	31
TTCTACG	45	0.008848979	17.77829	2
TAGCGGC	445	0.0	17.617746	30
CAAGACG	370	0.0	16.432907	4
GTTTTCG	540	0.0	16.296082	28
AAGACGG	380	0.0	16.000463	5
ATACCGT	480	0.0	16.000463	6
TACCGTC	450	0.0	16.000462	7
ACCGTCG	440	0.0	15.99979	8
GTATTAG	345	0.0	15.77056	1
TTTTCGG	565	0.0	15.575017	29
TTCGGAA	570	0.0	15.438393	31
CGCAAGA	405	0.0	15.407853	2
AGGCCCG	585	0.0	15.316038	10
TTTCGGA	575	0.0	15.304147	30
GTGTAAT	105	2.1814649E-6	15.240457	1
CGGCATC	400	0.0	15.1998	25
>>END_MODULE