FastQCFastQC Report
Thu 2 Feb 2017
SRR4062854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888899
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20120.22634742529803725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT13970.15716071229689763No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13160.14804831595040607No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12740.1433233696966697No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12330.1387109221632604No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12280.1381484285616251No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12090.13601095287541104No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11480.1291485309354606No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11320.1273485514102277No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11270.12678605780859242No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11150.12543607316466773No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10900.12262360515649134No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10510.11823615506373615No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10000.11249872032705627No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9600.10799877151397404No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9590.10788627279364696No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9330.1049613060651435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAC350.002054247322.8799111
ACACCGT507.203207E-419.1964236
CGAAAAC2700.018.37108223
GTCGTAA701.7876533E-518.28436530
TAATACT1251.4915713E-1017.9166624
GGCGTTA1352.3646862E-1117.77646631
CGTTATT1453.6379788E-1217.6588362
GTTATTC1550.017.5461043
GAGTAAG753.2048552E-517.0836681
CTAGCGG2250.017.06540729
CCGAAAA2950.016.81420922
GCGGCGT1651.8189894E-1216.48363129
TCTAGCG2350.016.33921828
TCCGAAA3050.016.26292421
GTTATAT805.570909E-516.0159381
TCTATAG703.698881E-415.997923
TTAATCC1102.021934E-715.997024
CAATACT805.6275232E-515.9970184
GTATATA1652.910383E-1115.5306071
CAAGACG2800.015.4256974
Produced by FastQC (version 0.11.5)