##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062854_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	888899
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259697670938994	32.0	32.0	32.0	32.0	32.0
2	30.922165510367318	32.0	32.0	32.0	32.0	32.0
3	30.975003909330532	32.0	32.0	32.0	32.0	32.0
4	31.024117475663715	32.0	32.0	32.0	32.0	32.0
5	30.978148248563674	32.0	32.0	32.0	32.0	32.0
6	34.626612247285685	36.0	36.0	36.0	32.0	36.0
7	34.55394819883924	36.0	36.0	36.0	32.0	36.0
8	34.53394255140348	36.0	36.0	36.0	32.0	36.0
9	34.6475651339466	36.0	36.0	36.0	32.0	36.0
10	34.40371853270169	36.0	36.0	36.0	32.0	36.0
11	34.63441178356596	36.0	36.0	36.0	32.0	36.0
12	34.469065664378064	36.0	36.0	36.0	32.0	36.0
13	34.53982510948938	36.0	36.0	36.0	32.0	36.0
14	34.45728817334703	36.0	36.0	36.0	32.0	36.0
15	34.41055395494876	36.0	36.0	36.0	32.0	36.0
16	34.426140652650076	36.0	36.0	36.0	32.0	36.0
17	34.34949977444007	36.0	36.0	36.0	32.0	36.0
18	34.37651071719059	36.0	36.0	36.0	32.0	36.0
19	34.36073839660074	36.0	36.0	36.0	32.0	36.0
20	34.34430908348418	36.0	36.0	36.0	32.0	36.0
21	34.31874149931544	36.0	36.0	36.0	32.0	36.0
22	34.297961860683834	36.0	36.0	36.0	32.0	36.0
23	34.25467347808919	36.0	36.0	36.0	32.0	36.0
24	34.24109600753292	36.0	36.0	36.0	32.0	36.0
25	34.21725977867002	36.0	36.0	36.0	32.0	36.0
26	34.165481117652284	36.0	36.0	36.0	32.0	36.0
27	34.16720122308609	36.0	36.0	36.0	32.0	36.0
28	34.162156780466624	36.0	36.0	36.0	32.0	36.0
29	34.12494220378243	36.0	36.0	36.0	32.0	36.0
30	34.10501305547649	36.0	36.0	36.0	32.0	36.0
31	34.121784364702854	36.0	36.0	36.0	32.0	36.0
32	34.07768711630905	36.0	36.0	36.0	32.0	36.0
33	34.04283501275173	36.0	36.0	36.0	32.0	36.0
34	34.05223203086065	36.0	36.0	36.0	32.0	36.0
35	33.99714928242691	36.0	36.0	36.0	32.0	36.0
36	33.95858022114999	36.0	36.0	36.0	32.0	36.0
37	33.96242317743636	36.0	36.0	36.0	32.0	36.0
38	33.37308850611824	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	2.0
10	3.0
11	8.0
12	3.0
13	4.0
14	125.0
15	265.0
16	330.0
17	388.0
18	431.0
19	591.0
20	804.0
21	1096.0
22	1618.0
23	2381.0
24	3648.0
25	5484.0
26	7894.0
27	11433.0
28	16376.0
29	22054.0
30	29591.0
31	40008.0
32	54946.0
33	82010.0
34	186760.0
35	420641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51561021759697	17.330269856286886	11.30727125287201	25.846848673244132
2	16.92245871026781	19.86026831994435	37.066602732725734	26.1506702370621
3	18.649351993636383	24.03389727532423	28.553458977973744	28.76329175306564
4	12.441808400290705	15.409608110935856	36.062704050292844	36.085879438480596
5	14.813398162668834	36.40080189178061	33.50001912480397	15.285780820746586
6	34.13312693498452	35.638364893080855	16.947089423284613	13.281418748650012
7	30.35541720712927	30.4844532393444	21.051435539920732	18.108694013605593
8	28.349467597410268	32.271415634017515	19.72370189954944	19.655414869022774
9	27.775577795377636	14.00827543379653	18.729637662898696	39.486509107927134
10	16.16977521478963	26.336128195407092	31.24853047554655	26.245566114256725
11	37.802120411662266	21.07001588508737	22.08375896067428	19.044104742576085
12	24.901167984051874	23.89934771994483	28.41254519722528	22.786939098778017
13	29.62649177701344	19.1185984525011	25.107758984415874	26.147150786069584
14	23.88223593316142	19.847372044985082	24.924394821045244	31.345997200808256
15	25.30647700255083	26.910903098694877	22.320639925106526	25.461979973647757
16	26.046624137892238	25.49003724080512	23.599081918520266	24.864256702782374
17	24.17789029535865	25.623066104078763	25.162194092827008	25.03684950773558
18	25.35413776693229	24.348792652070262	26.02410533172676	24.272964249270686
19	25.848550749910835	24.897759886773645	25.3082954280809	23.945393935234616
20	25.98883722395418	23.802071672627573	24.881672548818866	25.327418554599383
21	27.512333421462685	23.988659440059852	24.023536162098004	24.47547097637946
22	26.14078846852685	24.14717209117082	24.67404034710526	25.037999093197065
23	24.44446194574173	23.8990215149698	25.191742337900795	26.464774201387677
24	24.901861288286472	24.89601065714964	25.08446848703804	25.117659567525845
25	24.97755334877033	24.295602706649834	25.065652235551443	25.66119170902839
26	24.765824629229364	25.236819513095256	25.475354905784148	24.522000951891236
27	25.78271611605395	24.493827827206164	24.467951054647244	25.255505002092644
28	24.69075383498996	24.416130864989228	25.62127681117843	25.271838488842384
29	24.51998262869281	24.919387683923215	25.510957199529265	25.049672487854714
30	24.458389630961314	25.015722085282892	25.877708614352716	24.648179669403078
31	25.31760068042824	24.95522314176038	24.23991845661595	25.487257721195427
32	24.818752531389226	24.72143467890734	24.394941721794698	26.064871067908733
33	24.342082317481353	24.290103698470006	25.205017399336427	26.16279658471221
34	25.287607209279038	24.527592852827244	25.263192240315302	24.92160769757842
35	26.192367497845353	24.278053687197477	25.182103967469942	24.347474847487224
36	24.681786115714754	25.24164903030978	24.689211950192902	25.387352903782563
37	25.74574011464703	25.0065536697738	24.598594758189275	24.649111457389896
38	24.71862329047617	24.688471767824275	25.272826277727024	25.320078663972527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	305.0
1	247.0
2	189.0
3	189.0
4	396.5
5	604.0
6	604.0
7	728.5
8	853.0
9	776.5
10	700.0
11	700.0
12	900.5
13	1101.0
14	1506.0
15	1911.0
16	1911.0
17	2718.0
18	3525.0
19	3525.0
20	4327.0
21	5129.0
22	5513.5
23	5898.0
24	5898.0
25	7088.5
26	8279.0
27	8279.0
28	10486.5
29	12694.0
30	15820.5
31	18947.0
32	18947.0
33	24412.5
34	29878.0
35	29878.0
36	33376.0
37	36874.0
38	42752.0
39	48630.0
40	48630.0
41	51370.5
42	54111.0
43	61769.5
44	69428.0
45	69428.0
46	71594.5
47	73761.0
48	73761.0
49	78721.0
50	83681.0
51	84040.0
52	84399.0
53	84399.0
54	80384.0
55	76369.0
56	76369.0
57	74047.0
58	71725.0
59	64307.0
60	56889.0
61	56889.0
62	54340.5
63	51792.0
64	42533.0
65	33274.0
66	33274.0
67	27856.0
68	22438.0
69	22438.0
70	17974.0
71	13510.0
72	10629.5
73	7749.0
74	7749.0
75	5738.0
76	3727.0
77	3727.0
78	3720.5
79	3714.0
80	2930.5
81	2147.0
82	2147.0
83	2273.0
84	2399.0
85	2399.0
86	1568.0
87	737.0
88	655.0
89	573.0
90	573.0
91	413.0
92	253.0
93	208.0
94	163.0
95	163.0
96	153.5
97	144.0
98	144.0
99	271.5
100	399.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11463619601327034
2	0.05422438319764113
3	0.010799877151397403
4	0.002812468008176407
5	1.1249872032705627E-4
6	3.3749616098116883E-4
7	0.0
8	4.499948813082251E-4
9	3.3749616098116883E-4
10	6.749923219623377E-4
11	0.0016874808049058443
12	0.001912478245559957
13	0.01237485923597619
14	0.007987409143220997
15	0.01912478245559957
16	0.010012386109108009
17	0.016762309328731385
18	0.005624936016352814
19	0.006749923219623377
20	0.005849933457006926
21	0.006074930897661039
22	0.00697492066027749
23	0.008549902744856277
24	0.011924864354667965
25	0.014062340040882033
26	0.015974818286441993
27	0.007987409143220997
28	0.004949943694390477
29	0.008662401465183333
30	0.0024749718471952383
31	0.005287439855371646
32	0.006637424499296321
33	0.008099907863548052
34	0.011249872032705627
35	0.01361234515957381
36	0.01237485923597619
37	0.009449892507472727
38	0.006187429617988095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	888899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.2448956696635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.4873767118815	49.46200923029313
2	10.973839637136496	13.002879687955392
3	2.698391396421704	4.795977502707642
4	0.9743054734347871	2.3089050449610427
5	0.48363529466759325	1.4326461287374261
6	0.2892111711363378	1.0280571396284115
7	0.18674533675042165	0.7744595594802435
8	0.12841452750901944	0.6086324227792791
9	0.08030428539694687	0.4281857109152099
>10	0.5200604490345155	5.935249989980198
>50	0.07608372075008077	3.205095409800057
>100	0.08923785006492382	11.196339070043795
>500	0.009915316652515993	3.97657506411003
>1k	0.0024788291631289983	1.8449880386081368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2012	0.22634742529803725	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1397	0.15716071229689763	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1316	0.14804831595040607	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1274	0.1433233696966697	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1233	0.1387109221632604	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1228	0.1381484285616251	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1209	0.13601095287541104	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1148	0.1291485309354606	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1132	0.1273485514102277	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1127	0.12678605780859242	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1115	0.12543607316466773	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1090	0.12262360515649134	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1051	0.11823615506373615	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1000	0.11249872032705627	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	960	0.10799877151397404	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	959	0.10788627279364696	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	933	0.1049613060651435	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1249872032705628E-4
7	0.0	0.0	0.0	0.0	1.1249872032705628E-4
8	0.0	0.0	0.0	0.0	1.1249872032705628E-4
9	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
10	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
11	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
12	0.0	0.0	0.0	2.2499744065411257E-4	3.3749616098116883E-4
13	0.0	0.0	0.0	2.2499744065411257E-4	5.624936016352814E-4
14	0.0	0.0	0.0	2.2499744065411257E-4	7.87491042289394E-4
15	0.0	0.0	0.0	2.2499744065411257E-4	7.87491042289394E-4
16	0.0	0.0	0.0	2.2499744065411257E-4	7.87491042289394E-4
17	0.0	0.0	0.0	2.2499744065411257E-4	7.87491042289394E-4
18	0.0	0.0	0.0	2.2499744065411257E-4	7.87491042289394E-4
19	0.0	0.0	0.0	2.2499744065411257E-4	8.999897626164503E-4
20	0.0	0.0	0.0	2.2499744065411257E-4	0.0010124884829435066
21	0.0	0.0	0.0	3.3749616098116883E-4	0.0011249872032705627
22	0.0	0.0	0.0	4.4999488130822513E-4	0.0012374859235976191
23	0.0	0.0	0.0	5.624936016352814E-4	0.0012374859235976191
24	0.0	0.0	0.0	0.0011249872032705627	0.0012374859235976191
25	0.0	0.0	0.0	0.0013499846439246753	0.0012374859235976191
26	0.0	0.0	0.0	0.0021374756862140693	0.0012374859235976191
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAC	35	0.0020542473	22.879911	1
ACACCGT	50	7.203207E-4	19.196423	6
CGAAAAC	270	0.0	18.371082	23
GTCGTAA	70	1.7876533E-5	18.284365	30
TAATACT	125	1.4915713E-10	17.916662	4
GGCGTTA	135	2.3646862E-11	17.776466	31
CGTTATT	145	3.6379788E-12	17.658836	2
GTTATTC	155	0.0	17.546104	3
GAGTAAG	75	3.2048552E-5	17.083668	1
CTAGCGG	225	0.0	17.065407	29
CCGAAAA	295	0.0	16.814209	22
GCGGCGT	165	1.8189894E-12	16.483631	29
TCTAGCG	235	0.0	16.339218	28
TCCGAAA	305	0.0	16.262924	21
GTTATAT	80	5.570909E-5	16.015938	1
TCTATAG	70	3.698881E-4	15.99792	3
TTAATCC	110	2.021934E-7	15.99702	4
CAATACT	80	5.6275232E-5	15.997018	4
GTATATA	165	2.910383E-11	15.530607	1
CAAGACG	280	0.0	15.425697	4
>>END_MODULE