FastQCFastQC Report
Thu 2 Feb 2017
SRR4062854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888899
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23720.2668469646157775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17920.20159770682608483No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14120.15884819310180348No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13070.14703582746746258No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12850.14456085562026733No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12600.1417483876120909No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12560.14129839273078268No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12340.13882342088358746No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11730.131960998943637No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11650.13106100918102057No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11450.12881103477447944No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11220.12622356420695716No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11030.12408608852074307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10420.11722366658079265No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10240.11519868961490562No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10210.11486119345392447No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9770.109911249759534No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9000.10124884829435066No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8990.10113634957402359No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8900.1001238610910801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCGC350.00206653322.8564618
CGACAAT400.004483469819.99940119
TCTAGCG2750.018.03582428
ACTGCGC450.00884452717.7792478
TAGCGTA1800.017.7792477
TTACGAC450.008850262517.77724632
CTAGCGG2800.017.71375729
GTATTGA550.001363046717.456971
CGTGCGC753.231628E-517.06711810
GTATTAG2450.016.981951
ATAGCGT1900.016.8434966
GTCCTAC1051.1862903E-716.7642351
AGCGTAT1950.016.4116138
AATAGCG1950.016.4116135
TCTAGAT2050.016.3897552
CCGTCCA600.002439975716.0013229
TTAGGAC1001.3131885E-616.0004233
ACTATAC805.6172932E-516.0004223
GATAACG908.57427E-616.00042211
TAGCGGC3250.015.75337530
Produced by FastQC (version 0.11.5)