##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	888899
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23636881130477	32.0	32.0	32.0	32.0	32.0
2	31.319290493070643	32.0	32.0	32.0	32.0	32.0
3	31.395350877883764	32.0	32.0	32.0	32.0	32.0
4	31.489556181298436	32.0	32.0	32.0	32.0	32.0
5	31.423100937226838	32.0	32.0	32.0	32.0	32.0
6	34.946662106718534	36.0	36.0	36.0	36.0	36.0
7	34.96827648585497	36.0	36.0	36.0	36.0	36.0
8	34.91061751672575	36.0	36.0	36.0	36.0	36.0
9	35.02262574263218	36.0	36.0	36.0	36.0	36.0
10	34.85877473143743	36.0	36.0	36.0	32.0	36.0
11	35.03493985255918	36.0	36.0	36.0	36.0	36.0
12	34.93250526775258	36.0	36.0	36.0	32.0	36.0
13	34.984360427900135	36.0	36.0	36.0	36.0	36.0
14	34.93507473852485	36.0	36.0	36.0	32.0	36.0
15	34.90441321229971	36.0	36.0	36.0	32.0	36.0
16	34.91814368111563	36.0	36.0	36.0	32.0	36.0
17	34.867464132595494	36.0	36.0	36.0	32.0	36.0
18	34.8845268135075	36.0	36.0	36.0	32.0	36.0
19	34.87190783204841	36.0	36.0	36.0	32.0	36.0
20	34.85333429332241	36.0	36.0	36.0	32.0	36.0
21	34.84797260431163	36.0	36.0	36.0	32.0	36.0
22	34.83587111696605	36.0	36.0	36.0	32.0	36.0
23	34.77894901445496	36.0	36.0	36.0	32.0	36.0
24	34.74290779942378	36.0	36.0	36.0	32.0	36.0
25	34.72857433746691	36.0	36.0	36.0	32.0	36.0
26	34.678397658226636	36.0	36.0	36.0	32.0	36.0
27	34.659423624056274	36.0	36.0	36.0	32.0	36.0
28	34.614074264905234	36.0	36.0	36.0	32.0	36.0
29	34.58531509204083	36.0	36.0	36.0	32.0	36.0
30	34.569075901761614	36.0	36.0	36.0	32.0	36.0
31	34.55558280524559	36.0	36.0	36.0	32.0	36.0
32	34.53982173452777	36.0	36.0	36.0	32.0	36.0
33	34.491701531895075	36.0	36.0	36.0	32.0	36.0
34	34.48163402141301	36.0	36.0	36.0	32.0	36.0
35	34.44998363143619	36.0	36.0	36.0	32.0	36.0
36	34.423580181775435	36.0	36.0	36.0	32.0	36.0
37	34.41353629602463	36.0	36.0	36.0	32.0	36.0
38	33.979157362085004	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	16.0
22	69.0
23	180.0
24	508.0
25	1313.0
26	2906.0
27	5671.0
28	10054.0
29	16047.0
30	24365.0
31	35470.0
32	50749.0
33	81397.0
34	199656.0
35	460492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01049137906787	18.357044412567152	11.96050966163193	26.671954546733044
2	15.931506279116075	20.857150249915907	36.312674443328206	26.89866902763981
3	17.603124764455806	24.732393669022017	28.89867127761422	28.76581028890796
4	11.944578505721136	16.19703970201272	36.1985696880516	35.65981210421455
5	14.182601172911657	37.06079093350313	33.16046029976409	15.596147593821119
6	34.169911317258766	36.03817756573019	16.554974187168618	13.236936929842425
7	29.987242630763035	30.745597357627087	21.078008950408258	18.18915106120162
8	28.205431092090965	32.5065644258743	19.64478974712054	19.643214734914196
9	27.67026226734349	13.827087158440438	18.509130755661158	39.993519818554915
10	15.987994042020853	26.47768232811185	31.284565504020733	26.24975812584656
11	37.69821938660136	20.970218855578718	22.134643489178725	19.1969182686412
12	24.75652300108112	24.008288961663553	28.382493730966495	22.852694306288836
13	29.650983577418334	19.014217604269557	25.236641324426422	26.098157493885687
14	23.784254454105586	19.772437588522433	24.912616618985957	31.530691338386024
15	25.345736692245126	26.922518756349145	22.30815874469428	25.423585806711447
16	26.170044257046364	25.356340097514003	23.48132181645138	24.992293828988252
17	24.164950123692343	25.709332556342172	25.092614571509248	25.033102748456237
18	25.443160584048357	24.28397376979837	26.104090565969813	24.16877508018346
19	25.752307067507108	24.94085379778805	25.20421330207369	24.10262583263115
20	25.996766786777805	23.818566563805337	24.86367967564369	25.320986973773174
21	27.466112572969482	23.980789718516952	24.136262950008945	24.41683475850462
22	25.98337943905888	24.141100394983006	24.805292839793946	25.07022732616416
23	24.426227110677615	23.63524682837269	25.40069321867438	26.537832842275318
24	24.89236684932709	24.746568507783223	25.16011380370548	25.200950839184205
25	25.006131187155333	24.137752588035973	25.192316778753014	25.66379944605568
26	24.97440651233325	25.09748025082743	25.54556315797763	24.38255007886169
27	25.908342792600735	24.259674046207724	24.5086337142915	25.32334944690004
28	24.665569429147745	24.33471069266587	25.67693292488798	25.322786953298404
29	24.61100754978912	24.699656541406842	25.65589566418682	25.033440244617218
30	24.514821143909487	24.747693494986496	25.9955293008542	24.741956060249816
31	25.317049518561724	24.657582020004522	24.449909382280776	25.575459079152974
32	24.87166708478691	24.559258138438675	24.558808143557368	26.01026663321705
33	24.472409126346186	23.99923950865059	25.35777405532012	26.1705773096831
34	25.295224766818276	24.24302423559932	25.523034675480567	24.938716322101836
35	26.33538793496224	24.003064465141712	25.369923917115443	24.291623682780607
36	24.77919313667807	24.882916956819617	24.85569226650047	25.482197640001846
37	25.93489249059792	24.787405543261944	24.65949449825008	24.618207467890052
38	24.8020861809636	24.392027422749766	25.341518758641328	25.464367637645307
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	145.0
1	117.0
2	89.0
3	89.0
4	325.5
5	562.0
6	562.0
7	664.5
8	767.0
9	768.0
10	769.0
11	769.0
12	992.0
13	1215.0
14	1549.5
15	1884.0
16	1884.0
17	2896.5
18	3909.0
19	3909.0
20	4644.0
21	5379.0
22	5727.0
23	6075.0
24	6075.0
25	7062.5
26	8050.0
27	8050.0
28	10246.5
29	12443.0
30	15656.5
31	18870.0
32	18870.0
33	24451.5
34	30033.0
35	30033.0
36	33502.0
37	36971.0
38	42645.5
39	48320.0
40	48320.0
41	51162.0
42	54004.0
43	62122.0
44	70240.0
45	70240.0
46	71920.0
47	73600.0
48	73600.0
49	78651.0
50	83702.0
51	83931.0
52	84160.0
53	84160.0
54	80327.0
55	76494.0
56	76494.0
57	74318.0
58	72142.0
59	64554.5
60	56967.0
61	56967.0
62	54538.0
63	52109.0
64	42759.0
65	33409.0
66	33409.0
67	28068.5
68	22728.0
69	22728.0
70	18234.0
71	13740.0
72	10729.0
73	7718.0
74	7718.0
75	5740.5
76	3763.0
77	3763.0
78	3746.5
79	3730.0
80	2898.0
81	2066.0
82	2066.0
83	1989.5
84	1913.0
85	1913.0
86	1199.0
87	485.0
88	411.5
89	338.0
90	338.0
91	200.0
92	62.0
93	41.0
94	20.0
95	20.0
96	19.5
97	19.0
98	19.0
99	14.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008324905304202166
2	0.0
3	0.0
4	2.2499744065411254E-4
5	0.0
6	0.0
7	1.1249872032705627E-4
8	0.001912478245559957
9	0.00393745521144697
10	7.874910422893939E-4
11	0.005399938575698701
12	6.749923219623377E-4
13	1.1249872032705627E-4
14	0.0
15	0.0
16	1.1249872032705627E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.2499744065411254E-4
24	0.0
25	1.1249872032705627E-4
26	1.1249872032705627E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.2499744065411254E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	888899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.460657748535915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38797324386596	47.340671331613166
2	11.71199733601081	13.459581409525631
3	2.832630026293246	4.882943534071874
4	1.0654085146997854	2.4487629610216146
5	0.5464014943185586	1.5698294629163643
6	0.293668005054714	1.0124614038086543
7	0.1954962901217236	0.7863341792454989
8	0.14056772172149462	0.6461690998664192
9	0.09230304622694323	0.47734143735543066
>10	0.5441075583202782	6.044906880313586
>50	0.07947846314297215	3.217034539708859
>100	0.09560750254391434	11.474309711686919
>500	0.011213225585454664	4.298288079405644
>1k	0.0031475720941627127	2.3413659694603313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2372	0.2668469646157775	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1792	0.20159770682608483	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1412	0.15884819310180348	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1307	0.14703582746746258	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1285	0.14456085562026733	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1260	0.1417483876120909	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1256	0.14129839273078268	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1234	0.13882342088358746	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1173	0.131960998943637	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1165	0.13106100918102057	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1145	0.12881103477447944	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1122	0.12622356420695716	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1103	0.12408608852074307	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1042	0.11722366658079265	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1024	0.11519868961490562	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1021	0.11486119345392447	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	977	0.109911249759534	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	900	0.10124884829435066	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	899	0.10113634957402359	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	890	0.1001238610910801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.1249872032705628E-4
6	0.0	0.0	0.0	0.0	1.1249872032705628E-4
7	0.0	0.0	0.0	0.0	1.1249872032705628E-4
8	0.0	0.0	0.0	0.0	1.1249872032705628E-4
9	0.0	0.0	0.0	0.0	1.1249872032705628E-4
10	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
11	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
12	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
13	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
14	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
15	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
16	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
17	0.0	0.0	0.0	1.1249872032705628E-4	1.1249872032705628E-4
18	0.0	0.0	0.0	1.1249872032705628E-4	2.2499744065411257E-4
19	0.0	0.0	0.0	1.1249872032705628E-4	2.2499744065411257E-4
20	0.0	0.0	0.0	1.1249872032705628E-4	3.3749616098116883E-4
21	0.0	0.0	0.0	2.2499744065411257E-4	3.3749616098116883E-4
22	0.0	0.0	2.2499744065411257E-4	3.3749616098116883E-4	3.3749616098116883E-4
23	0.0	0.0	2.2499744065411257E-4	4.4999488130822513E-4	3.3749616098116883E-4
24	0.0	0.0	2.2499744065411257E-4	8.999897626164503E-4	3.3749616098116883E-4
25	0.0	0.0	2.2499744065411257E-4	0.0011249872032705627	3.3749616098116883E-4
26	0.0	0.0	2.2499744065411257E-4	0.002024976965887013	4.4999488130822513E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCCGC	35	0.002066533	22.85646	18
CGACAAT	40	0.0044834698	19.999401	19
TCTAGCG	275	0.0	18.035824	28
ACTGCGC	45	0.008844527	17.779247	8
TAGCGTA	180	0.0	17.779247	7
TTACGAC	45	0.0088502625	17.777246	32
CTAGCGG	280	0.0	17.713757	29
GTATTGA	55	0.0013630467	17.45697	1
CGTGCGC	75	3.231628E-5	17.067118	10
GTATTAG	245	0.0	16.98195	1
ATAGCGT	190	0.0	16.843496	6
GTCCTAC	105	1.1862903E-7	16.764235	1
AGCGTAT	195	0.0	16.411613	8
AATAGCG	195	0.0	16.411613	5
TCTAGAT	205	0.0	16.389755	2
CCGTCCA	60	0.0024399757	16.001322	9
TTAGGAC	100	1.3131885E-6	16.000423	3
ACTATAC	80	5.6172932E-5	16.000422	3
GATAACG	90	8.57427E-6	16.000422	11
TAGCGGC	325	0.0	15.753375	30
>>END_MODULE