FastQCFastQC Report
Thu 2 Feb 2017
SRR4062853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433094
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25550.17828558349975646No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17910.12497435618319525No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17180.11988048236891648No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16540.11541462039475428No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16440.11471682946129144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16070.11213500300747892No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15450.1078086992200093No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15280.10662245463312246No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15190.10599444279300589No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14830.10348239543253968No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14790.10320327905915454No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14710.10264504631238426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC604.7251197E-621.331668
ACGAACG1900.020.21176115
CGAACGA2050.019.51338816
GTTCGCA507.1958546E-419.2005049
CCGATAA2100.018.2861929
ATAACGA2150.017.86093312
TCTAGCG3550.017.57608428
CAAGACG4700.017.3579164
CTAGCGG3600.017.33257929
TCTTATA1900.016.844312
GATAACG2300.016.69608911
CGATAAC2300.016.69608910
GCGCAAG4800.016.6828231
AAGACGG4900.016.648855
CGCAAGA4850.016.4970042
TAACGAA2350.016.34142513
GTACCGT805.63291E-515.99651056
ATCGTTT3150.015.74534429
GACGGAC5100.015.6850427
AGACGGA5250.015.5394686
Produced by FastQC (version 0.11.5)