##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062853_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1433094
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206650087154088	32.0	32.0	32.0	32.0	32.0
2	30.734020238728235	32.0	32.0	32.0	32.0	32.0
3	30.78730006545279	32.0	32.0	32.0	32.0	32.0
4	30.872706884544908	32.0	32.0	32.0	32.0	32.0
5	30.73728380692404	32.0	32.0	32.0	32.0	32.0
6	34.406116416648175	36.0	36.0	36.0	32.0	36.0
7	34.33805179562541	36.0	36.0	36.0	32.0	36.0
8	34.306060174698935	36.0	36.0	36.0	32.0	36.0
9	34.50264602321969	36.0	36.0	36.0	32.0	36.0
10	34.13946677608028	36.0	36.0	36.0	32.0	36.0
11	34.4829425006315	36.0	36.0	36.0	32.0	36.0
12	34.25912954767796	36.0	36.0	36.0	32.0	36.0
13	34.3712387324209	36.0	36.0	36.0	32.0	36.0
14	34.252630322923686	36.0	36.0	36.0	32.0	36.0
15	34.18558238329098	36.0	36.0	36.0	32.0	36.0
16	34.20279060550111	36.0	36.0	36.0	32.0	36.0
17	34.11344126763492	36.0	36.0	36.0	32.0	36.0
18	34.15014437294413	36.0	36.0	36.0	32.0	36.0
19	34.137870230424525	36.0	36.0	36.0	32.0	36.0
20	34.13178409790286	36.0	36.0	36.0	32.0	36.0
21	34.11300654388337	36.0	36.0	36.0	32.0	36.0
22	34.0853021504521	36.0	36.0	36.0	32.0	36.0
23	34.01998403454344	36.0	36.0	36.0	32.0	36.0
24	33.99447768255258	36.0	36.0	36.0	32.0	36.0
25	33.968610572649105	36.0	36.0	36.0	32.0	36.0
26	33.917108717223016	36.0	36.0	36.0	32.0	36.0
27	33.9245318171732	36.0	36.0	36.0	32.0	36.0
28	33.90993333305422	36.0	36.0	36.0	32.0	36.0
29	33.87401384696329	36.0	36.0	36.0	32.0	36.0
30	33.836954868278006	36.0	36.0	36.0	32.0	36.0
31	33.86527890005819	36.0	36.0	36.0	32.0	36.0
32	33.81630025664751	36.0	36.0	36.0	32.0	36.0
33	33.78637828362969	36.0	36.0	36.0	32.0	36.0
34	33.785116677621986	36.0	36.0	36.0	32.0	36.0
35	33.74954469141591	36.0	36.0	36.0	32.0	36.0
36	33.692886858782465	36.0	36.0	36.0	27.0	36.0
37	33.692596577754145	36.0	36.0	36.0	27.0	36.0
38	33.08834731008573	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	1.0
9	4.0
10	13.0
11	21.0
12	7.0
13	10.0
14	127.0
15	270.0
16	391.0
17	474.0
18	581.0
19	859.0
20	1193.0
21	1812.0
22	2725.0
23	4598.0
24	6936.0
25	10640.0
26	15616.0
27	22510.0
28	31451.0
29	41936.0
30	56082.0
31	75384.0
32	100407.0
33	148440.0
34	315207.0
35	595396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.752396494223404	16.973578135980876	11.263208486963714	26.010816882832007
2	16.977040656037108	19.27897913789709	37.39302604516468	26.350954160901114
3	19.136754510725666	23.345718317447332	28.11870602504819	29.398821146778808
4	12.452103937682608	15.0432189782499	36.1343036640008	36.3703734200667
5	14.757094300625154	36.44544957842755	33.661250154387	15.136205966560299
6	34.775676250885326	35.3491942208592	16.67654047038382	13.198589057871654
7	30.825843123928454	30.14347289394338	20.642972926390684	18.387711055737483
8	28.4491354701132	32.80615117951337	19.216923419378112	19.52778993099531
9	27.515508446782828	14.208807541745458	18.333112365587645	39.942571645884065
10	15.705374892975943	26.41782889967057	31.69412367270979	26.182672534643697
11	37.843075136699724	21.107930677399818	21.888354216268873	19.16063996963159
12	24.799064931439936	23.683332751823034	28.524475768465862	22.99312654827117
13	29.505422300138395	19.21606779079043	25.175259041412428	26.10325086765875
14	23.594673678730516	19.73168806644154	24.884661738402844	31.788976516425105
15	25.3433066458583	26.941902918638682	22.254366930330825	25.460423505172198
16	26.144734114901553	25.55642944426494	23.497572458129525	24.801263982703983
17	24.328847701384078	25.771550331275122	25.042417034653198	24.857184932687602
18	25.356662349407678	24.61556390473745	25.84298203499213	24.184791710862743
19	25.639560160421276	25.159510585305977	25.11959368503472	24.08133556923803
20	25.632166742963054	24.254644775186183	24.94166117704856	25.171527304802204
21	26.973119971639857	24.22656851340233	24.26641507274614	24.533896442211674
22	25.841442862277	24.248613907333294	24.880790796833182	25.02915243355653
23	24.486877705533296	24.048828180883678	25.441975012264965	26.022319101318057
24	24.891093918148407	25.128167907286414	24.9209637054169	25.059774469148277
25	25.064957996271765	24.426300328925098	24.99628362793288	25.512458046870258
26	24.836685696737902	25.335143283873755	25.4537904272693	24.37438059211904
27	25.63092825520179	24.65644731773494	24.55267668224273	25.15994774482054
28	24.57064061794534	24.66708117964532	25.543490017843595	25.218788184565742
29	24.56869930415449	25.01610333490819	25.5034254916315	24.911771869305824
30	24.71047644036052	24.99448725928862	25.703467583688987	24.591568716661875
31	25.120306712332972	24.954082472903078	24.61926135586502	25.30634945889893
32	24.89684207294223	24.87667454991183	24.504447671558278	25.722035705587658
33	24.479246992092662	24.454892032159712	25.212059124631097	25.853801851116526
34	25.371480254250773	24.758324982064543	25.228624706542462	24.64157005714222
35	26.02497658532364	24.414074767842262	25.23097130079519	24.329977346038905
36	24.69008026542515	25.161511399142	24.891982599968873	25.25642573546397
37	25.808540690825655	24.933633506216538	24.66600416482897	24.59182163812884
38	24.697243122460744	24.770795213950052	25.235485325171425	25.29647633841778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	81.0
2	116.0
3	116.0
4	297.5
5	479.0
6	479.0
7	617.0
8	755.0
9	779.0
10	803.0
11	803.0
12	1091.0
13	1379.0
14	1865.5
15	2352.0
16	2352.0
17	3450.0
18	4548.0
19	4548.0
20	5845.5
21	7143.0
22	8391.0
23	9639.0
24	9639.0
25	11753.5
26	13868.0
27	13868.0
28	17988.0
29	22108.0
30	27005.5
31	31903.0
32	31903.0
33	40327.0
34	48751.0
35	48751.0
36	54597.5
37	60444.0
38	69444.0
39	78444.0
40	78444.0
41	84076.5
42	89709.0
43	101730.0
44	113751.0
45	113751.0
46	117865.5
47	121980.0
48	121980.0
49	128647.0
50	135314.0
51	134501.5
52	133689.0
53	133689.0
54	128440.0
55	123191.0
56	123191.0
57	119007.0
58	114823.0
59	102925.5
60	91028.0
61	91028.0
62	85768.5
63	80509.0
64	67193.0
65	53877.0
66	53877.0
67	44938.5
68	36000.0
69	36000.0
70	28950.0
71	21900.0
72	17117.5
73	12335.0
74	12335.0
75	9462.5
76	6590.0
77	6590.0
78	6196.0
79	5802.0
80	4514.5
81	3227.0
82	3227.0
83	3179.5
84	3132.0
85	3132.0
86	2117.0
87	1102.0
88	943.0
89	784.0
90	784.0
91	580.5
92	377.0
93	330.0
94	283.0
95	283.0
96	264.5
97	246.0
98	246.0
99	456.5
100	667.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11360036396775089
2	0.05135741270286527
3	0.012281120428946041
4	0.0030005010138902262
5	4.884536534239904E-4
6	6.28011840116559E-4
7	6.977909334628433E-5
8	2.09337280038853E-4
9	2.791163733851373E-4
10	3.4889546673142163E-4
11	0.0018142564270033927
12	0.0030702801072365104
13	0.01500250506945113
14	0.009420177601748386
15	0.02351555445769782
16	0.011373992215444346
17	0.020724390723846447
18	0.0060010020277804525
19	0.008164153921515267
20	0.0060010020277804525
21	0.006768572054589581
22	0.007605921174744992
23	0.010536643095288935
24	0.014514051416027142
25	0.01709587786983966
26	0.01911947157688191
27	0.008792165761631825
28	0.006070781121126737
29	0.011234434028751777
30	0.003488954667314217
31	0.00628011840116559
32	0.006768572054589581
33	0.008443270294900404
34	0.01242067861563861
35	0.016188749656337965
36	0.015560737816221406
37	0.009908631255172376
38	0.006698792961243296
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1433094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.13619769899236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2971695797681	44.51441588500384
2	12.816188973914516	14.389042359746226
3	3.8354477077361637	6.45922352356873
4	1.5045032996948935	3.3782837868183555
5	0.7337687073792427	2.0595492611387622
6	0.4241018108472148	1.4284477858931948
7	0.2679656166958924	1.0529799584761115
8	0.18618126380718064	0.8361206586342516
9	0.12370044530969387	0.6249665388022531
>10	0.650233764560843	6.730774380175393
>50	0.07045616476696102	2.7830840682222204
>100	0.07729600162577446	9.37769885510858
>500	0.00936538261560562	3.568422159556012
>1k	0.0036212812780341726	2.7969907788560175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2555	0.17828558349975646	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1791	0.12497435618319525	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1718	0.11988048236891648	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1654	0.11541462039475428	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1644	0.11471682946129144	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1607	0.11213500300747892	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1545	0.1078086992200093	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1528	0.10662245463312246	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1519	0.10599444279300589	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1483	0.10348239543253968	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1479	0.10320327905915454	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1471	0.10264504631238426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.7911637338513736E-4
13	0.0	0.0	0.0	0.0	3.488954667314217E-4
14	0.0	0.0	0.0	0.0	3.488954667314217E-4
15	0.0	0.0	0.0	0.0	6.977909334628434E-4
16	6.977909334628434E-5	0.0	0.0	6.977909334628434E-5	6.977909334628434E-4
17	6.977909334628434E-5	0.0	0.0	1.3955818669256868E-4	6.977909334628434E-4
18	6.977909334628434E-5	0.0	0.0	2.09337280038853E-4	7.675700268091277E-4
19	6.977909334628434E-5	0.0	0.0	2.09337280038853E-4	7.675700268091277E-4
20	6.977909334628434E-5	0.0	0.0	2.09337280038853E-4	9.071282135016963E-4
21	6.977909334628434E-5	0.0	0.0	3.488954667314217E-4	0.001046686400194265
22	6.977909334628434E-5	0.0	0.0	4.18674560077706E-4	0.001046686400194265
23	6.977909334628434E-5	0.0	0.0	0.001046686400194265	0.001256023680233118
24	6.977909334628434E-5	0.0	0.0	0.0015351400536182554	0.001256023680233118
25	6.977909334628434E-5	0.0	0.0	0.001953814613695961	0.001256023680233118
26	6.977909334628434E-5	0.0	0.0	0.0021631518937348143	0.0013258027735794024
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	60	4.7251197E-6	21.33166	8
ACGAACG	190	0.0	20.211761	15
CGAACGA	205	0.0	19.513388	16
GTTCGCA	50	7.1958546E-4	19.200504	9
CCGATAA	210	0.0	18.286192	9
ATAACGA	215	0.0	17.860933	12
TCTAGCG	355	0.0	17.576084	28
CAAGACG	470	0.0	17.357916	4
CTAGCGG	360	0.0	17.332579	29
TCTTATA	190	0.0	16.84431	2
GATAACG	230	0.0	16.696089	11
CGATAAC	230	0.0	16.696089	10
GCGCAAG	480	0.0	16.682823	1
AAGACGG	490	0.0	16.64885	5
CGCAAGA	485	0.0	16.497004	2
TAACGAA	235	0.0	16.341425	13
GTACCGT	80	5.63291E-5	15.9965105	6
ATCGTTT	315	0.0	15.745344	29
GACGGAC	510	0.0	15.685042	7
AGACGGA	525	0.0	15.539468	6
>>END_MODULE